Methylene blue-mediated photodynamic inactivation as a novel disinfectant of enterovirus 71

Wong, Tak Wah; Huang, Hsuan Jung; Wang, Ya Fang; Lee, Yi Ping; Huang, Chien Chun; Yu, Chun Keung

doi:10.1093/jac/dkq301

Cited by 29 publications

(26 citation statements)

References 20 publications

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“…In fact, although it has been shown that both enveloped and nonenveloped viruses can be efficiently inactivated due to the damages induced by photodynamic procedure on viral proteins Malik et al, 1993;Moor et al, 1997;Smetana et al, 1998;Hotze et al, 2009;Wong et al, 2010), there is still scarce information about the type of damage induced to phage proteins and which are the most affected proteins. For this reason, it is important to pursue in the development of novel, convenient and fast-preparation methods to evaluate and characterize the damages induced by photosensitization on viral molecular structures.…”

Section: Discussionmentioning

confidence: 99%

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Costa

Esteves

Correia

et al. 2014

Journal of Virological Methods

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Section: Discussionmentioning

confidence: 99%

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Costa

Esteves

Correia

et al. 2014

Journal of Virological Methods

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“…However, while the same treatment is reported to be ineffective against some non-enveloped viruses [83,131], the results from Wong et al [85] showed that even a non-enveloped virus can be efficiently inactivated due to the damage induced by PDI to its viral proteins (Table 4). …”

Section: Molecular Targets Of Antiviral Pdimentioning

confidence: 99%

“…The protein profile of the non-enveloped enterovirus 71 was considerably altered after a low dose PDI and a MB concentration ≥0.5 μM, as revealed by a smearing and the disappearance of several protein bands [85]. However, enterovirus 71 PDI was also due to damages in the viral genome [85].…”

Section: Molecular Targets Of Antiviral Pdimentioning

confidence: 99%

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Costa

Faustino

Neves

et al. 2012

Viruses

200

205

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Photodynamic inactivation (PDI) has been used to inactivate microorganisms through the use of photosensitizers. The inactivation of mammalian viruses and bacteriophages by photosensitization has been applied with success since the first decades of the last century. Due to the fact that mammalian viruses are known to pose a threat to public health and that bacteriophages are frequently used as models of mammalian viruses, it is important to know and understand the mechanisms and photodynamic procedures involved in their photoinactivation. The aim of this review is to (i) summarize the main approaches developed until now for the photodynamic inactivation of bacteriophages and mammalian viruses and, (ii) discuss and compare the present state of the art of mammalian viruses PDI with phage photoinactivation, with special focus on the most relevant mechanisms, molecular targets and factors affecting the viral inactivation process.

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“…Our related study showed that whole body exposures to EV71 viral suspension are an effective and less stressful alternative for inducing the lethal infection of newborn mice via the oral route [75]. In laboratory mice, fecal-oral transmission of EV71 between infected and non-infected littermates occurs after close contact [6,15].…”

Section: Reviewmentioning

confidence: 99%

Animal models of enterovirus 71 infection: applications and limitations

Wang

2014

J Biomed Sci

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Human enterovirus 71 (EV71) has emerged as a neuroinvasive virus that is responsible for several outbreaks in the Asia-Pacific region over the past 15 years. Appropriate animal models are needed to understand EV71 neuropathogenesis better and to facilitate the development of effective vaccines and drugs. Non-human primate models have been used to characterize and evaluate the neurovirulence of EV71 after the early outbreaks in late 1990s. However, these models were not suitable for assessing the neurovirulence level of the virus and were associated with ethical and economic difficulties in terms of broad application. Several strategies have been applied to develop mouse models of EV71 infection, including strategies that employ virus adaption and immunodeficient hosts. Although these mouse models do not closely mimic human disease, they have been applied to determine the pathogenesis of and treatment and prevention of the disease. EV71 receptor-transgenic mouse models have recently been developed and have significantly advanced our understanding of the biological features of the virus and the host-parasite interactions. Overall, each of these models has advantages and disadvantages, and these models are differentially suited for studies of EV71 pathogenesis and/or the pre-clinical testing of antiviral drugs and vaccines. In this paper, we review the characteristics, applications and limitation of these EV71 animal models, including non-human primate and mouse models.

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Methylene blue-mediated photodynamic inactivation as a novel disinfectant of enterovirus 71

Abstract: Methylene blue-mediated photodynamic inactivation may provide a novel way to eliminate environmentally contaminated sources of EV71 to prevent infection.

Cited by 29 publications

References 20 publications

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Animal models of enterovirus 71 infection: applications and limitations

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