Methylglyoxal‐augmented manuka honey as a topical anti–<i>Staphylococcus aureus</i> biofilm agent: safety and efficacy in an in vivo model

Paramasivan, S.; Drilling, Amanda; Jardeleza, Camille; Jervis‐Bardy, Josh; Vreugde, Sarah; Wormald, Peter John

doi:10.1002/alr.21264

Cited by 61 publications

(63 citation statements)

References 34 publications

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“…No epithelial damage of the nasal respiratory mucosa was seen on light microscopy or transmission electron microscopy. Paramasivan et al 797 performed both a safety and efficacy study in an in vivo sheep model by performing frontal trephinations and irrigating the frontal sinuses with Manuka honey. In the safety arm, they found that a concentration of MGO up to 1.8 mg/mL did not cause any epithelial injury on standard microscopy and scanning electron microscopy; however, at an MGO concentration of 3.6 mg/mL, patchy ciliary denudation of the epithelium was demonstrated.…”

Section: S93mentioning

confidence: 99%

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Orlandi

Kingdom

Hwang

et al. 2016

Int Forum Allergy Rhinol

558

888

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Background:The body of knowledge regarding rhinosinusitis (RS) continues to expand, with rapid growth in number of publications, yet substantial variability in the quality of those presentations. In an effort to both consolidate and critically appraise this information, rhinologic experts from around the world have produced the International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICAR:RS). Methods:Evidence-based reviews with recommendations (EBRRs) were developed for scores of topics, using previously reported methodology. Where existing evidence was insufficient for an EBRR, an evidence-based review (EBR) was produced. The sections were then synthesized and the entire manuscript was then reviewed by all authors for consensus. Results:The resulting ICAR:RS document addresses multiple topics in RS, including acute RS (ARS), chronic RS (CRS) with and without nasal polyps (CRSwNP and CRSsNP), recurrent acute RS (RARS), acute exacerbation of CRS (AE-CRS), and pediatric RS. Conclusion:As a critical review of the RS literature, ICAR:RS provides a thorough review of pathophysiology and evidence-based recommendations for medical and surgical treatment. It also demonstrates the significant gaps in our understanding of the pathophysiology and optimal management of RS. Too o en the foundation upon which these recommendations are based is comprised of lowerlevel evidence. It is our hope that this summary of the evidence in RS will point out where additional research efforts may be directed. C 2016 ARS-AAOA, LLC. Key Words:rhinosinusitis; chronic rhinosinusitis; acute rhinosinusitis; recurrent acute rhinosinusitis; evidence-based medicine; systematic review; endoscopic sinus surgery List of Abbreviations Used

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Section: S93mentioning

confidence: 99%

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Orlandi

Kingdom

Hwang

et al. 2016

Int Forum Allergy Rhinol

558

888

View full text Add to dashboard Cite

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“…Topical delivery of antimicrobials to the nose and paranasal sinuses in nebulizers and irrigations has been reported to be beneficial against biofilm-associated chronic rhinosinusitis (9,10). Another promising approach is the use of gels that can be directly instilled into the sinuses.…”

mentioning

confidence: 99%

A Topical Hydrogel with Deferiprone and Gallium-Protoporphyrin Targets Bacterial Iron Metabolism and Has Antibiofilm Activity

Richter

Thomas

Claeys

et al. 2017

Antimicrob Agents Chemother

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Many infectious diseases are associated with multidrug-resistant (MDR) bacteria residing in biofilms that require high antibiotic concentrations. While oral drug delivery is frequently ineffective, topical treatments have the potential to deliver higher drug concentrations to the infection site while reducing systemic side effects. This study determined the antibiofilm activity of a surgical wound gel loaded with the iron chelator deferiprone (Def) and the heme analogue gallium-protoporphyrin (GaPP), alone and in combination with ciprofloxacin. Activity against MDR Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Acinetobacter johnsonii biofilms was assessed in the colony biofilm and artificial wound model by enumeration of CFU and correlative light/electron microscopy. While Staphylococcus biofilms were equally susceptible to GaPP and Def-GaPP gels (log 10 reduction of 3.8 and 3.7, respectively), the Def-GaPP combination was crucial for significant activity against P. aeruginosa biofilms (log 10 reduction of 1.3 for GaPP and 3.3 for DefGaPP). When Def-GaPP gel was combined with ciprofloxacin, the efficacy exceeded the activity of the individual compounds. Def-GaPP delivered in a surgical wound gel showed significant antibiofilm activity against different MDR strains and could enhance the gel's wound-healing properties. Moreover, Def-GaPP indicated a potentiation of ciprofloxacin. This antibiofilm strategy has potential for clinical utilization as a therapy for topical biofilm-related infections.KEYWORDS antimicrobial combinations, biofilms, drug delivery, iron metabolism M edical treatments for chronic infectious diseases are typically based on oral delivery of high-dose, long-term antibiotic therapies. Despite the risk for emerging antimicrobial resistance and the potential of side effects (e.g., gastrointestinal disorders, neutropenia, nephrotoxicity), there is a lack of suitable alternatives. Depending on the disease nature and localization, topical treatments can deliver high dosages of antimicrobials directly to an infection site while reducing unwanted systemic effects. Higher drug dosages are particularly needed to combat microbial biofilms (1). The ability of bacteria to form biofilms and establish resistance to antibiotics is a major biomedical threat, adding billions of dollars to health care costs worldwide (1-3). Biofilms are responsible for 80% of microbial infections in humans and are a common cause of chronic infections, including chronic wound and chronic sinus infections (4, 5), with increasing tolerance and subtle resistance mechanisms to antibiotic therapies (6-8).

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“…Honey through different studies has almost equal or slightly superior effects when compared with conventional treatments for acute wounds [34] especially pan-or multidrug-resistant bacterial infections [5]. Consequently; honey is getting worldwide attention as a topical therapeutic agent for wound infection and potential future candidate for systemic infections [42].…”

Section: Discussionmentioning

confidence: 99%

Honey and Medicinal Plants in the Management of Certain Surgical Bovine Clow Affections

Ma¹,

As²,

Mm³

et al. 2017

IJCAM

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Owing to the worldwide spread of bacterial antimicrobial resistance, it is of great concern to search for antimicrobial agents of natural origin. Since the antimicrobial activity of honey was widely documented, the study aimed to evaluate its use comparing with certain medicinal plants extracts as surgical dressing of bovine clow affections. Aqueous extracts of Thymus vulgaris (T. vulgaris), Matricaria chamomilla (M. chamomilla) and Origanum vulgare (O. vulgare) were prepared to be used in vitro and in vivo studies in this work. Clinical aerobic or anaerobic bacterial strains were isolated from some clinical surgical bovine clow affections, and were used to determine the minimal inhibitory concentrations (MICs) of the prepared extracts against these pathogens.The study concluded that all tested aerobic bacterial strains were inhibited with 10 % of all tested extracts, while the anaerobic strains were inhibited with 10 % of T. vulgaris and 15 % of both O. vulgare and M. chamomilla extracts. According the achieved MICs values, lotions and ointments of the entended medicinal plants were made to be used during the in vivo study as follow: 20 lactating dairy cows suffering from different surgical clow affections were classified to equal four groups (A -D) which were managed with surgical dressing and received: honey (A): T. vulgaris (B), M. chamomilla: (C) and O. vulgare: (D) extracts with their MICs. All cows of groups A & B (no = 10)as well as one cow from group (C) showed complete healing by the day 30 while, none of group (D).The study concluded that with the alternative medical trends, application of honey -as it is -in surgical dressing of bovine digital dermatitis or inter digital necrobacillosis is the most effective valuable economic tool among the four materials studied for its superurity and feasibility followed by the use of T. vulgaris extract 10% in lotion and ointment preparation.

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Methylglyoxal‐augmented manuka honey as a topical anti–Staphylococcus aureus biofilm agent: safety and efficacy in an in vivo model

Abstract: Sinus irrigation with MH/MGO at MGO concentrations between 0.9 and 1.8 mg/mL is both safe to mucosa and efficacious against S. aureus biofilm. MH/MGO irrigation could represent a viable treatment option for recalcitrant CRS.

Cited by 61 publications

References 34 publications

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

A Topical Hydrogel with Deferiprone and Gallium-Protoporphyrin Targets Bacterial Iron Metabolism and Has Antibiofilm Activity

Honey and Medicinal Plants in the Management of Certain Surgical Bovine Clow Affections

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