Methylglyoxal destroys Agrobacterium tumefaciens crown gall tumours in Nicotiana tabacum without any adverse effect on the host plant

Ray, Anamika; Roy, Chaitali; Ray, Subhankar; Mazumder, Mina; Sengupta, Dibyendu N.; Ray, Manju

doi:10.1007/s10535-011-0044-4

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…The procedures described above as well as studies carried out in our laboratory earlier indicate a significant role of MG in the process of differentiation (Roy et al 2004, Ray et al 2011. These studies have shown that the Chl content of calluses increases with the concentration of MG from 0.1 to 0.25 mM MG and then begins to drop at 0.5 mM MG.…”

Section: Resultsmentioning

confidence: 70%

“…As a ketoaldehyde, MG exhibits strong anticancer and other diverse biological effects (Kalapos 2008, Talukdar et al 2008. Similar to its effect on animal systems, MG was found to destroy crown gall tumors completely in Nicotiana tabacum without any adverse effect on the host plant (Ray et al 2011).…”

Section: Introductionmentioning

confidence: 92%

“…Methylglyoxal was also observed to play a significant role in the formation of chlorophyll (Chl) in calluses (Roy et al 2004, Ray et al 2011). It has also been observed that MG has a very strong positive effect in cell to cell adhesion thereby resulting in organogenesis (Lieber 1995).…”

Section: Introductionmentioning

confidence: 99%

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Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

Ray¹,

Ray²,

Mukhopadhyay³

et al. 2013

Biologia plant.

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The aim of this study was to evaluate the influence of methylglyoxal (MG) on organogenesis and regeneration of tobacco (Nicotiana tabacum L.) plants from callus in media containing glycine or succinate. The best improvement in shoot proliferation and shoot length was obtained in the medium supplemented with 0.1 mM MG and 0.5 mM glycine or 0.25 mM succinate. The histological studies showed vigorous development of corm like structures and shoot organogenesis from callus tissues cultured in MG supplemented media. Biochemical studies also revealed higher content of δ-aminolaevulinic acid (a precursor of chlorophyll) and of chlorophyll.

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Section: Resultsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 92%

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Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

Ray¹,

Ray²,

Mukhopadhyay³

et al. 2013

Biologia plant.

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“…Before infecting the shoot, the site was pinched with sterile needle. After 15 days the gall started forming (Ray, 2011).…”

Section: Formation Of Crowngall In Tobaccomentioning

confidence: 99%

Expression of Bacterial Protein-a in Tobacco Leads to Enhanced Resistance to Stress Conditions

Roy¹,

Ray²,

Begum³

et al. 2014

J microb biotech food sci

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Tobacco is the most commonly used plant for expression of transgenes from a variety of organisms because it can be easily grown and transformed, it provides abundant amounts of fresh tissue and has a well-established cell culture system. As bacterial enzymes can be synthesized in tobacco, here we explore the possibility of in planta expression of staphylococcal protein-A(PA) which is an antibody, an important group among biopharmaceuticals. In our study we have shown that the tobacco plants harboring PA gene could combat the crown gall infection and also effective in resisting abiotic stress conditions. Transgenic plants when subjected to interact with wild variety of Agrobacterium shows its enhanced capability to resist the gall formation. And when transgenic tobacco plants were grown in presence of 200mM NaCl and/or MG(Methylglyoxal) solution, shows their increased tolerance towards salinity stress and high MG stress. So far transgenic tobacco plants are concerned, improvements in the expression of recombinant proteins and their recovery from tobacco may also enhance production and commercial use of this protein.

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“…6,7 On the other hand, MGX is also considered an inhibitor of cell growth, especially for rapidly proliferating cells, hence some authors considered it as a potent anticancer agent. 8,9 Nevertheless, there are not any clear studies correlating exogenous MGX uptake and endogenous intracellular AGE effects or any antineoplastic processes.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic analysis of methylglyoxal and other α-dicarbonyls using gas-diffusion microextraction

Santos

Valente

Gonçalves

et al. 2013

Analyst

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Many α-dicarbonyl compounds such as methylglyoxal, diacetyl and pentane-2,3-dione are important quality markers of processed foods. They are produced by enzymatic and chemical processes, the Maillard reaction is the most known chemical route for α-dicarbonyl formation. In the case of methylglyoxal, there are obstacles to be overcome when analysing this compound due to its high reactivity, low volatility and low concentration. The use of extraction techniques based on the volatilization of methylglyoxal (like solid-phase microextraction) showed to be ineffective for the methylglyoxal extraction from aqueous solutions. Therefore, derivatization is typically applied to increase analyte's volatility. In this work a new methodology for the extraction and analysis of methylglyoxal and also diacetyl and pentane-2,3-dione from selected food matrices is presented. It is based on a gas-diffusion microextraction step followed by high performance liquid chromatographic analysis. It was successfully applied to port wines, black tea and soy sauce. Methylglyoxal, diacetyl and pentane-2,3-dione were quantified in the following concentration ranges: 0.24-1.74 mg L(-1), 0.1-1.85 mg L(-1) and 0.023-0.15 mg L(-1), respectively. The main advantages over existing methodologies are its simplicity in terms of sample handling, not requiring any chemical modification of the α-dicarbonyls prior to the extraction, low reagent consumption and short time of analysis.

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Methylglyoxal destroys Agrobacterium tumefaciens crown gall tumours in Nicotiana tabacum without any adverse effect on the host plant

Cited by 3 publications

References 26 publications

Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

Expression of Bacterial Protein-a in Tobacco Leads to Enhanced Resistance to Stress Conditions

Chromatographic analysis of methylglyoxal and other α-dicarbonyls using gas-diffusion microextraction

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