Methylglyoxal-modified collagen promotes myofibroblast differentiation

Yuen, Amy; Laschinger, Carol; Talior, Ilana; Wilson, Lionel; Chan, Man Khun; Birek, Juliana; Young, Elton T.; Sivagurunathan, Koneswaran; Won, Emily; Simmons, Craig A.; McCulloch, C

doi:10.1016/j.matbio.2010.04.004

Cited by 69 publications

(67 citation statements)

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“…Our study could explain why the fibrosis rate is higher in diabetes, for example, in the pathogenesis of diabetic nephropathy (Burns et al ., 2006). Furthermore, the similar effects of TGFβ1 and TGFβ2 on the AGE enhancement of EMT further suggests that basement membrane AGEs could play a role in TGFβ1‐mediated fibrosis, such as the cardiac and lung fibroses that occur in the diseases of these organs (Yuen et al ., 2010; Song et al ., 2011). …”

Section: Discussionmentioning

confidence: 99%

“…The AGE–RAGE interaction also enhances TGFβ1 expression and induces fibrosis of the peritoneal membrane (De Vriese et al ., 2003). AGE‐modified collagen promotes cardiac fibroblast differentiation into myofibroblasts (Yuen et al ., 2010), and AGE blockade reduces bleomycin‐induced pulmonary fibrosis in rats (Chen et al ., 2009). Taken together, these observations support a role for AGEs in fibrotic diseases.…”

Section: Introductionmentioning

confidence: 99%

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AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

et al. 2016

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SummaryProteins in basement membrane (BM) are long‐lived and accumulate chemical modifications during aging; advanced glycation endproduct (AGE) formation is one such modification. The human lens capsule is a BM secreted by lens epithelial cells. In this study, we have investigated the effect of aging and cataracts on the AGE levels in the human lens capsule and determined their role in the epithelial‐to‐mesenchymal transition (EMT) of lens epithelial cells. EMT occurs during posterior capsule opacification (PCO), also known as secondary cataract formation. We found age‐dependent increases in several AGEs and significantly higher levels in cataractous lens capsules than in normal lens capsules measured by LC‐MS/MS. The TGFβ2‐mediated upregulation of the mRNA levels (by qPCR) of EMT‐associated proteins was significantly enhanced in cells cultured on AGE‐modified BM and human lens capsule compared with those on unmodified proteins. Such responses were also observed for TGFβ1. In the human capsular bag model of PCO, the AGE content of the capsule proteins was correlated with the synthesis of TGFβ2‐mediated α‐smooth muscle actin (αSMA). Taken together, our data imply that AGEs in the lens capsule promote the TGFβ2‐mediated fibrosis of lens epithelial cells during PCO and suggest that AGEs in BMs could have a broader role in aging and diabetes‐associated fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

et al. 2016

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“…Recently, Shao et al (56) showed that MG reduced the ability of sarco(endo) plasmic reticulum Ca 2ϩ -ATPase 2a to transport Ca 2ϩ and induced diastolic dysfunction, which may play a role in development of diabetic cardiomyopathy. In addition, MG has been reported to affect cardiac fibroblasts function by promoting myofibroblast differentiation (74), but the mechanism by which this occurs and the molecular targets of MG are unclear.…”

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confidence: 99%

Effects of methylglyoxal on human cardiac fibroblast: roles of transient receptor potential ankyrin 1 (TRPA1) channels

Oguri

Nakajima

Yamamoto

et al. 2014

American Journal of Physiology-Heart and Circulatory Physiology

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Effects of methylglyoxal on human cardiac fibroblast: roles of transient receptor potential ankyrin 1 (TRPA1) channels. Am J Physiol Heart Circ Physiol 307: H1339 -H1352, 2014. First published August 29, 2014; doi:10.1152/ajpheart.01021.2013.-Cardiac fibroblasts contribute to the pathogenesis of cardiac remodeling. Methylglyoxal (MG) is an endogenous carbonyl compound produced under hyperglycemic conditions, which may play a role in the development of pathophysiological conditions including diabetic cardiomyopathy. However, the mechanism by which this occurs and the molecular targets of MG are unclear. We investigated the effects of MG on Ca 2ϩ signals, its underlying mechanism, and cell cycle progression/cell differentiation in human cardiac fibroblasts. The conventional and quantitative real-time RT-PCR, Western blot, immunocytochemical analysis, and intracellular Ca 2ϩ concentration [Ca 2ϩ ]i measurement were applied. Cell cycle progression was assessed using the fluorescence activated cell sorting. MG induced Ca 2ϩ entry concentration dependently. Ruthenium red (RR), a general cation channel blocker, and HC030031, a selective transient receptor potential ankyrin 1 (TRPA1) antagonist, inhibited MG-induced Ca 2ϩ entry. Treatment with aminoguanidine, a MG scavenger, also inhibited it. Allyl isothiocyanate, a selective TRPA1 agonist, increased Ca 2ϩ entry. The use of small interfering RNA to knock down TRPA1 reduced the MGinduced Ca 2ϩ entry as well as TRPA1 mRNA expression. The quantitative real-time RT-PCR analysis showed the prominent existence of TRPA1 mRNA. Expression of TRPA1 protein was confirmed by Western blotting and immunocytochemical analyses. MG promoted cell cycle progression from G0/G1 to S/G2/M, which was suppressed by HC030031 or RR. MG also enhanced ␣-smooth muscle actin expression. The present results suggest that methylglyoxal activates TRPA1 and promotes cell cycle progression and differentiation in human cardiac fibroblasts. MG might participate the development of pathophysiological conditions including diabetic cardiomyopathy via activation of TRPA1.human cardiac fibroblast; transient receptor potential ankyrin 1 channels; methylglyoxal CARDIAC FIBROBLASTS ARE THE predominant secretary cell types located within the extracellular matrix (ECM) (18), which account for 60ϳ70% of the cells in human hearts and play a key role in regulating normal myocardial function and in the adverse myocardial remodeling that occurs with hypertension, heart failure, and myocardial infarction (12, 71). Many of the functional effects of cardiac fibroblasts are mediated through differentiation of cardiac fibroblasts to myofibroblasts phenotype that expresses contractile proteins such as ␣-smooth muscle actin (␣-SMA), and they consequently exhibit increased migratory and proliferative properties. Cardiac fibroblasts also take a part in the maintenance of myocardial function by producing the type I and type III collagens and by secreting growth factors (48), and a key source of components of the ECM that regulat...

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“…For example, in Refs. [58,156] it was concluded that the glycation of collagen under diabetes mellitus facilitates the development of fibrosis. In turn, the structural changes of tissue proteins lead to the change of their optical properties.…”

Section: Different Tissuesmentioning

confidence: 99%

Optical and structural properties of biological tissues under diabetes mellitus

Tuchina

Tuchin

2018

J-BPE

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Abstract. Diabetes mellitus is a serious social and economic problem of modern society because it is widespread and fraught with numerous complications. Therefore, it is necessary to search for new methods of diabetes mellitus diagnostics and treatment and to improve the existing ones, which, in turn, requires thorough investigation of the disease development mechanisms, as well as elaboration of simple and reliable methods and criteria for detecting the complication precursors. In connection with the solution of these problems, in the paper we present an analytical review of recent publications devoted to the study of the changes of structural and optical properties of biological tissues under the conditions of diabetes mellitus development using in vitro models of glycated tissues, in vivo experimental models of diabetes in laboratory animals, and clinical studies.

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Methylglyoxal-modified collagen promotes myofibroblast differentiation

Cited by 69 publications

References 53 publications

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

Effects of methylglyoxal on human cardiac fibroblast: roles of transient receptor potential ankyrin 1 (TRPA1) channels

Optical and structural properties of biological tissues under diabetes mellitus

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