Methylglyoxal synthase regulates cell elongation via alterations of cellular methylglyoxal and spermidine content in Bacillus subtilis

Shin, Sang-Min; Song, Sung-Hyun; Lee, Jin Woo; Kwak, Min-Kyu; Kang, Sa-Ouk

doi:10.1016/j.biocel.2017.08.005

Cited by 9 publications

(6 citation statements)

References 68 publications

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“…The pathways of MGO formation rely on enzymatic (cytochrome P450, monooxygenase and methylglyoxal synthase) and non-enzymatic (autoxidation of sugars, ketone bodies and lipid peroxidation) mechanisms [113,114]. Currently, non-enzymatic conversion of triose phosphate isomers—dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP), is considered to be the main route of MGO formation in plants (Figure 3) [115].…”

Section: Possible Role Of Protein Glycation In Plant Physiologymentioning

confidence: 99%

Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?

Shumilina

Kusnetsova

Tsarev

et al. 2019

IJMS

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Glycation can be defined as an array of non-enzymatic post-translational modifications of proteins formed by their interaction with reducing carbohydrates and carbonyl products of their degradation. Initial steps of this process rely on reducing sugars and result in the formation of early glycation products—Amadori and Heyns compounds via Schiff base intermediates, whereas their oxidative degradation or reactions of proteins with α-dicarbonyl compounds yield a heterogeneous group of advanced glycation end products (AGEs). These compounds accompany thermal processing of protein-containing foods and are known to impact on ageing, pathogenesis of diabetes mellitus and Alzheimer’s disease in mammals. Surprisingly, despite high tissue carbohydrate contents, glycation of plant proteins was addressed only recently and its physiological role in plants is still not understood. Therefore, here we summarize and critically discuss the first steps done in the field of plant protein glycation during the last decade. We consider the main features of plant glycated proteome and discuss them in the context of characteristic metabolic background. Further, we address the possible role of protein glycation in plants and consider its probable contribution to protein degradation, methylglyoxal and sugar signalling, as well as interplay with antioxidant defense.

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Section: Possible Role Of Protein Glycation In Plant Physiologymentioning

confidence: 99%

Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?

Shumilina

Kusnetsova

Tsarev

et al. 2019

IJMS

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“…S7L). However, endogenous levels of methylglyoxal (an inducer of the GbaA regulon ( 33 , 34 )) can influence cell length in B. subtilis ( 77 ), while in C. crescentus cell cycle changes in metabolism and redox potential influence cell division and chromosome segregation ( 78 , 79 ). Therefore, further investigation into the fluctuations driving GbaA and NemR activity may illuminate new ways in which bacterial metabolism connects to cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

Transcription-replication interactions reveal principles of bacterial genome regulation

Pountain

Jiang

Yao

et al. 2022

Preprint

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Regulation of gene activity during the cell cycle is fundamental to bacterial replication but is challenging to study in unperturbed, asynchronous bacterial populations. Using single cell RNA-sequencing of heterogeneousStaphylococcus aureuspopulations, we uncovered a global gene expression pattern dominated by chromosomal position. We show that this pattern results from the effect of DNA replication on gene expression, and inEscherichia coli, changes under different growth rates and modes of replication. By constructing a quantitative model in each species that links replication to cell cycle gene expression, we identified divergent genes that may be instead subject to distinct regulation, and applied this cell cycle framework to characterize heterogeneity in responses to antibiotic stress. Our approach reveals a highly dynamic cell cycle transcriptional landscape and may be broadly applicable across species.

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“…Furthermore, P3 and E. coli but not P2 genome encode genes for MG synthesis (one MGS gene each). It is likely that MG serves physiological functions in these microbes such as the regulation of cell elongation in Bacillus (Shin et al, 2017) but a tight control needs to be maintained over its levels, to prevent cellular toxicity. Nonetheless, we did not observe similar elongation effect in P3 in response to exogenous MG, probably because MG concentration (1 mM MG) used here was fivefold higher than the critical concentration (0.2 mM) known to cause such effect (Shin et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Microbial methylglyoxal metabolism contributes towards growth promotion and stress tolerance in plants

Kaur

Gupta

Garai

et al. 2021

Environmental Microbiology

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Plant growth promotion by microbes is a cumulative phenomenon involving multiple traits, many of which are not explored yet. Hence, to unravel microbial mechanisms underlying growth promotion, we have analysed the genomes of two potential growthpromoting microbes, viz., Pseudomonas sp. CK-NBRI-02 (P2) and Bacillus marisflavi CK-NBRI-03 (P3) for the presence of plant-beneficial traits. Besides known traits, we found that microbes differ in their ability to metabolize methylglyoxal (MG), a ubiquitous cytotoxin regarded as general consequence of stress in plants. P2 exhibited greater tolerance to MG and possessed better ability to sustain plant growth under dicarbonyl stress. However, under salinity, only P3 showed a dose-dependent induction in MG detoxification activity in accordance with concomitant increase in MG levels, contributing to enhanced salt tolerance. Furthermore, saltstressed transcriptomes of both the strains showed differences with respect to MG, ion and osmolyte homeostasis, with P3 being more responsive to stress. Importantly, application of either strain altered MG levels and subsequently MG detoxification machinery in Arabidopsis, probably to strengthen plant defence response and growth. We therefore, suggest a crucial role of microbial MG resistance in plant growth promotion and that it should be considered as a beneficial trait while screening microbes for stress mitigation in plants.

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Methylglyoxal synthase regulates cell elongation via alterations of cellular methylglyoxal and spermidine content in Bacillus subtilis

Cited by 9 publications

References 68 publications

Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?

Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?

Transcription-replication interactions reveal principles of bacterial genome regulation

Microbial methylglyoxal metabolism contributes towards growth promotion and stress tolerance in plants

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