Methylisothiazolinone toxicity and inhibition of wound healing and regeneration in planaria

Huizen, Alanna V. Van; Tseng, Ai Sun; Beane, Wendy S.

doi:10.1016/j.aquatox.2017.08.013

Cited by 25 publications

(21 citation statements)

References 54 publications

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“…To assess the role of ROS during wound healing, we amputated planarians in half (bisection) and observed wound morphology every 15 minutes following injury. Consistent with our previous findings 41 , we observed that most animals (70%; n  44) close their wounds by one hour post amputation (Fig. 1A,C), where a lack of wound closure was defined as mesenchymal tissue protruding from the wound site.…”

Section: Ros Accumulate At the Wound Site Soon After Injurysupporting

confidence: 91%

“…We found that DPI concentrations as low as 5 µM and up to 50 µM inhibited wound closure (Fig. 2C), although at higher concentrations, animals were increasingly immobile-a sign of toxicity (see our previous work 41 for examples). We chose 15 µM DPI as our working concentration, as this dose blocked wound healing without affecting normal movement.…”

Section: Ros Are Required For Wound Closuresupporting

confidence: 52%

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Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Huizen

Hack

Greene

et al. 2022

Preprint

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Reactive oxygen species (ROS), such as hydrogen peroxide, are conserved and critical components of both wound healing and regeneration. Even though millions are affected each year by poor wound healing and an inability to restore functional tissue, how the same ROS-mediated signaling regulates these two different processes is not fully understood. Here, we investigate the role(s) of ROS during planarian wound healing and regeneration. We show ROS accumulate after injury and are required for wound closure (by promoting cytoskeletal movements) and regrowth (by promoting blastema formation). We found that different threshold levels of ROS regulate separate downstream targets to control wound healing (jun-1) versus regeneration (hsp70). By only manipulating ROS levels, we were able to control which injury-induced program was initiated: failure to close (chronic wound), healing only (no blastema), or full regeneration. Our results demonstrate that healing versus regenerative outcomes are based on differential ROS-mediated gene expression soon after injury. This study highlights ROS signaling as a potential therapeutic means to control wound repair mechanisms in multiple contexts. Therefore, investigating the mechanisms by which ROS control different tissue repair processes will be necessary not only for regenerative medicine but to improve clinical outcomes for chronic wounds and fibrosis.

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Section: Ros Accumulate At the Wound Site Soon After Injurysupporting

confidence: 91%

Section: Ros Are Required For Wound Closuresupporting

confidence: 52%

Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Huizen

Hack

Greene

et al. 2022

Preprint

Self Cite

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“…For instance, an acute oral study showed that the median lethal doses (LD50) for male and female rats were 274.6 mg and 105.7 mg/kg of body weight, respectively (Burnett et al 2010). Aquatic toxicity studies showed that MIT is very toxic to aquatic life (Santos et al 2016;Van Huizen et al 2017;Kucera 2019). Van Huizen et al (2017) investigated the effects of MIT exposure on five different planarian species, which are common flatworms found in freshwater streams, lakes, ponds, and rivers.…”

Section: Human and Environmental Health Hazardsmentioning

confidence: 99%

“…Aquatic toxicity studies showed that MIT is very toxic to aquatic life (Santos et al 2016;Van Huizen et al 2017;Kucera 2019). Van Huizen et al (2017) investigated the effects of MIT exposure on five different planarian species, which are common flatworms found in freshwater streams, lakes, ponds, and rivers. The researchers found that MIT concentrations higher than 4.5 mg/L are lethal to planarians.…”

Section: Human and Environmental Health Hazardsmentioning

confidence: 99%

The application of non-oxidizing biocides to prevent biofouling in reverse osmosis polyamide membrane systems: a review

Da-Silva-Correa

Smith

Thibodeau

et al. 2022

Journal of Water Supply: Research and Technology-Aqua

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Biofouling of polyamide membranes is one of the main barriers faced by reverse osmosis (RO) technologies to supply fresh water. Currently, biofouling is addressed by feed water pretreatment using chlorine, followed by membrane cleaning. Chlorine damages polyamide membranes and also generates harmful disinfection byproducts. Thus, safer strategies are needed to prevent biofouling in polyamide membrane systems. This review investigates the applicability of the following non-oxidizing biocides in preventing and controlling biofouling in RO systems, including their antimicrobial efficiency, hazard levels, membrane compatibility, and applicability to drinking water treatment: (1) 2,2-dibromo-3-nitropropionamide (DBNPA); (2) 2-methyl-4-isothiazolin-3-one (MIT); (3) sodium bisulfite (SBS), (4) phenoxyethanol (PE), (5) sodium benzoate (SB). According to this review, MIT and DBNPA present most of the features attributed to an ideal anti-biofouling chemical but also are the most hazardous biocides. Due to safety and efficacy, none of the five chemicals were determined to be the final solution to address membrane biofouling. However, alternative RO biocide research is in early development and requires further investigation via biofouling prevention studies. Therefore, future research efforts on the investigation of economic, eco-friendly, and safe antifouling agents to prevent and treat biofouling in RO systems are paramount to promote sustainable water supply in water-stressed countries.

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“…[15] This organism is used in investigations linked with regeneration due to its ability to produce a new and complete organism from its fragments. [16] It has been used in neuropharmacological studies mainly focusing on drug addiction behavior due to the presence of neurotransmitters, [17][18][19][20][21] as well as in toxicological investigations [22][23][24] and environmental monitoring. [25] The use of planaria is linked with its unique advantages that include 1) easy and cheap maintenance in the laboratory; 2) greater numbers of organisms in assays, enabling significant results to be obtained; 3) greater number of pharmacological assays may be performed; and 4) the use of a whole animal instead of cell culture.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Polysaccharide Fraction from Yerba Mate (Ilex paraguariensisSt. Hil.) on Regeneration of Planarian (Girardia tigrina)

et al. 2020

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The present study evaluates the regenerative activity of a polysaccharide fraction from yerba mate (Ilex paraguariensis St. Hil.) using planarian (Girardia tigrina) as a model. At 10 mg L–1, the polysaccharide fraction improves the regeneration of the head, pharynx, and tail of G. tigrina. At the end of experiment, at 10 mg L–1, the planarians obtained are 22–72% higher than the control group. The planarian fragments exposed to the highest concentration used, i.e., (60 mg mL–1), show signs of toxicity, producing death of some organisms. Thus, the polysaccharide fraction presents a great potential for improving the regeneration of planarian segments, suggesting its potential use in tissue regeneration.

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Methylisothiazolinone toxicity and inhibition of wound healing and regeneration in planaria

Cited by 25 publications

References 54 publications

Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

The application of non-oxidizing biocides to prevent biofouling in reverse osmosis polyamide membrane systems: a review

Analysis of Polysaccharide Fraction from Yerba Mate (Ilex paraguariensisSt. Hil.) on Regeneration of Planarian (Girardia tigrina)

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