Methylmercury drives lipid droplet formation and adipokine expression during the late stages of adipocyte differentiation in 3T3-L1 cells

Takanezawa, Yasukazu; Kashiwano, Yui; Nakamura, Ryosuke; Ohshiro, Yuka; Uraguchi, Shimpei; Kiyono, Masako

doi:10.1016/j.tox.2023.153446

Cited by 6 publications

(6 citation statements)

References 43 publications

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“…Furthermore, GsMT×4 and other ion channel-modulating peptides are derived from venom and cause pain because due to their inherent toxicity (Suchyna et al, 2000). Other Piezo1 antagonists like ruthenium red and gadolinium contain heavy metals that are toxic and can accumulate in adipose tissue (Mabuza et al, 2018;Blythe et al, 2019;Takanezawa et al, 2023). Yoda1 has poor solubility and stability while Dooku1, Jedi1/2, Yoda2, and Yaddle1 are newer modulators and additional studies are needed to determine potential adverse reactions (Wang et al, 2018;Parsonage et al, 2022;Goon et al, 2024).…”

Section: Disadvantages Of Using Molecules To Modulate Piezo1 In Micro...mentioning

confidence: 99%

Microglial Piezo1 mechanosensitive channel as a therapeutic target in Alzheimer’s disease

Ikiz,

Hascup,

Bae

et al. 2024

Front. Cell. Neurosci.

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Microglia are the resident macrophages of the central nervous system (CNS) that control brain development, maintain neural environments, respond to injuries, and regulate neuroinflammation. Despite their significant impact on various physiological and pathological processes across mammalian biology, there remains a notable gap in our understanding of how microglia perceive and transmit mechanical signals in both normal and diseased states. Recent studies have revealed that microglia possess the ability to detect changes in the mechanical properties of their environment, such as alterations in stiffness or pressure. These changes may occur during development, aging, or in pathological conditions such as trauma or neurodegenerative diseases. This review will discuss microglial Piezo1 mechanosensitive channels as potential therapeutic targets for Alzheimer’s disease (AD). The structure, function, and modulation of Piezo1 will be discussed, as well as its role in facilitating microglial clearance of misfolded amyloid-β (Aβ) proteins implicated in the pathology of AD.

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Section: Disadvantages Of Using Molecules To Modulate Piezo1 In Micro...mentioning

confidence: 99%

Microglial Piezo1 mechanosensitive channel as a therapeutic target in Alzheimer’s disease

Ikiz,

Hascup,

Bae

et al. 2024

Front. Cell. Neurosci.

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“…The alterations in adipocyte phenotype, lipid peroxidation, and hormone secretions in response to MeHg were attenuated by the antioxidant theaflavin-3,3′-digallate [ 234 ], suggesting an important role for oxidative stress in the development of MeHg-induced metabolic dysfunction in vitro. In a model of 3T3-L1 adipocyte differentiation where MeHg was continually supplied for 8 days, MeHg enhanced the development of the lipid droplet, accumulating more triglycerides in MeHg-exposed cells than in untreated cells [ 236 ]. Furthermore, markers of mature adipocyte differentiation were significantly increased in the MeHg-treated cells, which included expression of PPARγ, adiponectin, and fatty acid binding protein [ 236 ].…”

Section: Introductionmentioning

confidence: 99%

“…In a model of 3T3-L1 adipocyte differentiation where MeHg was continually supplied for 8 days, MeHg enhanced the development of the lipid droplet, accumulating more triglycerides in MeHg-exposed cells than in untreated cells [ 236 ]. Furthermore, markers of mature adipocyte differentiation were significantly increased in the MeHg-treated cells, which included expression of PPARγ, adiponectin, and fatty acid binding protein [ 236 ]. MeHg was also shown to increase autophagy markers in this study.…”

Section: Introductionmentioning

confidence: 99%

“…MeHg was also shown to increase autophagy markers in this study. Interestingly, treatment of 3T3-L1 cells early in development with an autophagy inhibitor chloroquine prevented the effects of MeHg on the lipid droplet; however, chloroquine had no effect on MeHg-induced alterations if the cells received the inhibitor during late stages of development [ 236 ]. This suggests that early induction of autophagy in pre-adipocytes can drive the differentiation into mature adipocytes in response to MeHg.…”

Section: Introductionmentioning

confidence: 99%

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Association between Heavy Metals, Metalloids and Metabolic Syndrome: New Insights and Approaches

Martins

Ferrer

Tinkov

et al. 2023

Toxics

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Metabolic syndrome (MetS) is an important public health issue that affects millions of people around the world and is growing to pandemic-like proportions. This syndrome is defined by the World Health Organization (WHO) as a pathologic condition characterized by abdominal obesity, insulin resistance, hypertension, and hyperlipidemia. Moreover, the etiology of MetS is multifactorial, involving many environmental factors, including toxicant exposures. Several studies have associated MetS with heavy metals exposure, which is the focus of this review. Environmental and/or occupational exposure to heavy metals are a major risk, contributing to the development of chronic diseases. Of particular note, toxic metals such as mercury, lead, and cadmium may contribute to the development of MetS by altering oxidative stress, IL-6 signaling, apoptosis, altered lipoprotein metabolism, fluid shear stress and atherosclerosis, and other mechanisms. In this review, we discuss the known and potential roles of heavy metals in MetS etiology as well as potential targeted pathways that are associated with MetS. Furthermore, we describe how new approaches involving proteomic and transcriptome analysis, as well as bioinformatic tools, may help bring about an understanding of the involvement of heavy metals and metalloids in MetS.

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“…also see9,10). The final recommendations have been sent to the European Commission, which issued a final legally binding decision applicable in all EU Member States (see below):…”

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confidence: 99%

Gadolinium-Based Contrast Media Nephrotoxicity. Overview and Recommendations

Djendov,

Minev

2023

Biomedical Reviews

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Gadolinium-based contrast media (GBCM), gadolinium-based contrast agents (GBCAs), or simply gadolinium contrast agents, are molecular complexes containing the rare earth metal gadolinium chelated to a carrier ligand. These are a type of paramagnetic contrast agent, which are the primary class of magnetic resonance imaging (MRI) contrast media. The intravenous route of administration is the most common. Since the USA Food and Drug Administration requires warning labels on certain GBCAs that say the product shouldn't be given to people with kidney disease because it's a known cause of nephrogenic systemic fibrosis (NSF), and the EU healthcare authorities also warns for some health risks. We need substantiated basic and clinically data and Guideline on the use of Gadolinium-containing MRI Contrast Agents. The newer GBCAs on the market aren't known to cause NSF in people with kidney disease.

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Methylmercury drives lipid droplet formation and adipokine expression during the late stages of adipocyte differentiation in 3T3-L1 cells

Cited by 6 publications

References 43 publications

Microglial Piezo1 mechanosensitive channel as a therapeutic target in Alzheimer’s disease

Microglial Piezo1 mechanosensitive channel as a therapeutic target in Alzheimer’s disease

Association between Heavy Metals, Metalloids and Metabolic Syndrome: New Insights and Approaches

Gadolinium-Based Contrast Media Nephrotoxicity. Overview and Recommendations

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