Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium

Доронина, Н. В.; Ivanova, E. G.; Trotsenko, Yu. A.; Pshenichnikova, A. B.; Kalinina, E. S.; Швец, В. И.

doi:10.1016/j.syapm.2005.02.002

Cited by 39 publications

(13 citation statements)

References 15 publications

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“…can live in the cytoplasm of Acanthamoeba isolated from domestic tap water in Korea (Choi et al 2009). The isolated Methylophilus bacteria are strictly aerobic, obligate methylotrophs using methanol as the growth substrate, and can form EPS (Doronina et al 2005;Gogleva et al 2010). Hong et al (2010) speculated that Methylophilus spp.…”

Section: Discussionmentioning

confidence: 99%

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Liu

Zhang

et al. 2012

Can. J. Microbiol.

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In this study, to give insight into the bacterial diversity of biofilms from full-scale drinking water distribution systems (DWDSs), the bacterial community compositions of biofilms from two urban DWDSs (Guangzhou and Beijing, China) were determined using a 16S rRNA gene library technique. Meanwhile, the occurrence and diversity of mycobacteria were also analyzed by a Mycobacterium -specific hsp gene assay. The biofilms from the full-scale DWDSs have complex bacterial populations. Proteobacteria was the common and predominant group in all biofilm samples, in agreement with previous reports. The community structures of bacteria at the three sites in Guangzhou DWDS were significantly different, despite the similar physicochemical properties of portable water. Some abundant and peculiar bacterial phylotypes were noteworthy, including Methylophilus , Massilia , and Planomicrobium , members of which are rarely found in DWDSs and their roles in DWDS biofilms are still unclear. The diversity of Mycobacterium species in biofilm samples was rather low. Mycobacterium arupense and Mycobacterium gordonae were the primary Mycobacterium species in Guangzhou and Beijing biofilms, respectively, indicating that M. arupense may be more resistant to chloride than M. gordonae.

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Section: Discussionmentioning

confidence: 99%

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Liu

Zhang

et al. 2012

Can. J. Microbiol.

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“…Some species of Methylophilus have been linked to degradation or utilization of various compounds including dichloromethane (Nikolausz et al, 2006), methylated amines (Large and Haywood, 1981), methanol (Jenkins et al, 1987), glucose, and galactose (Doronina and Trotsenko, 1994). Two recent reports have also linked Methylophilus to utilization of biphenyl (Uhlik et al, 2009), phenol, and humic matter (Hutalle-Schmelzer et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Zhang

Wang

Wan

et al. 2011

J. Zhejiang Univ. Sci. B

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Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using terminal restriction fragment length polymorphism (TRFLP) and 16S rRNA gene clone library analysis. The two bimolecular tools revealed a large shift of bacterial community structure after anthracene amendment and subsequent biodegradation. Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium playing an active role.

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“…quaylei' VKM B-2338 (Doronina et al, 2005); 7, M. rhizosphaerae CBMB127 T (Madhaiyan et al, 2009). No glutamate or NADP + -specific isocitrate dehydrogenases activities were observed for all strains and diphosphatidylglycerol was absent in the phospholipid profile; these characteristics were not determined for M. rhizosphaerae CBMB127 T .…”

Section: Description Of Methylophilus Glucosoxydans Sp Novmentioning

confidence: 99%

Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere

Доронина

Gogleva

Trotsenko

2012

International Journal of Systematic and Evolutionary Microbiology

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Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere Two restricted facultatively methylotrophic strains, designed B T and P, were isolated from rice roots. The isolates were strictly aerobic, Gram-negative, asporogenous, mesophilic, neutrophilic, motile rods that multiplied by binary fission and were able to synthesize indole-3-acetate. The cellular fatty acid profiles of the two strains were dominated by C 16 : 0 , C 16 : 1 v7c and C 16 : 0 2-OH. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. Cardiolipin (diphosphatidylglycerol) was absent. The two strains assimilated methanol carbon at the level of formaldehyde via the ribulose monophosphate cycle (2-keto-3-deoxy-6-phosphogluconate variant). They lacked a-ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains B T and P were 52.5 and 51.5 mol% (T m ), respectively. The level of DNA-DNA reassociation between these strains was 78 %, indicating that they belong to one species. Phylogenetic analysis of strain B T based on 16S rRNA and methanol dehydrogenase (mxaF) gene sequences showed a high level of similarity to members of the genus Methylophilus. As the two isolates were clearly distinct from all recognized members of the genus Methylophilus based on phenotypic data and levels of DNA-DNA relatedness (30-46 %), they are considered to represent a novel species, for which the name Methylophilus glucosoxydans sp. nov. is proposed; the type strain is B T (5VKM B-1607The genus Methylophilus comprises non-halophilic, obligately and restricted facultatively methylotrophic bacteria having the ribulose monophosphate ( The ability to grow on methanol is common to the type strains of these species. No NADP + -specific isocitrate dehydrogenase or glutamate dehydrogenase activities were observed for any Methylophilus species and diphosphatidylglycerol was absent from their phospholipid profile. However, these metabolic characteristics were not determined for M. rhizosphaerae CBMB127 T (Madhaiyan et al., 2009).We previously isolated two colourless restricted facultatively methylotrophic strains, designated B T and P, from the rhizosphere of rice (Oryza sativa L.) sampled from Vietnam (Doronina et al., 1988). Here, we describe the characterization of these strains, and show that they represent a single novel species of the genus Methylophilus. Strains BT and P were grown on 'medium K', containing (per litre): 2.0 g KH 2 PO 4 , 2.0 g (NH 4 ) 2 SO 4 , 0.025 g MgSO 4 . 7H 2 O, 0.5 g NaCl and 0.002 g FeSO 4 . 7H 2 O (pH 7.2). Solid medium K was prepared by adding 2.0 % (w/v) agar (Difco).

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Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium

Cited by 39 publications

References 15 publications

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere

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