Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Carvalho, Beatriz Ferreira; Ávila, Carla Luiza da Silva; Pereira, Marcos Neves; Schwan, Rosane Freitas

doi:10.1111/gfs.12248

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…Using 100 g of glycerin kg −1 of DM, the level of DM) and cut at two ages (45 and 60 days) of DM losses was 22.39% (from 231.03 to 144.34 g kg −1 DM). These values are consistent with the results reported by Carvalho et al (2017), comparing different levels of inclusion in sugarcane silage. These authors reported reductions of 20.28% of DM losses by adding Lactobacillus hilgardii and 80 g kg −1 DM of glycerin.…”

Section: Discussionsupporting

confidence: 93%

“…This is a rich source of energy for anaerobic microorganisms (Santos et al, 2014;Carvalho et al, 2017), which can favor microbial growth and improve the quality of fermentation and forage conservation.…”

Section: Introductionmentioning

confidence: 99%

“…This has been associated to better fermentation, lower production of ethanol, lower dry matter (DM) losses, and silage with superior nutritional value (Santos et al, 2014;Carvalho et al, 2017). The inclusion of crude glycerin has not been tested in the ensiling of grass.…”

Section: Introductionmentioning

confidence: 99%

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The use of crude glycerin as an alternative to reduce fermentation losses and enhance the nutritional value of Piatã grass silage

et al. 2017

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-The objective of this study was to evaluate fermentation losses and the nutritional value of silage of Brachiaria brizantha cv BRS Piatã ensiled with different levels of crude glycerin. The experiment followed a 4 × 2 completely randomized factorial design with four doses of crude glycerin (0, 100, 200, and 300 g kg −1 of DM silage) and two cutting ages (45 and 60 days) with four replicates per treatment (silos). The parameters of dry matter losses, effluents losses, gas losses, pH, microbial population, and nutritional value of the silage were evaluated. There was no difference in pH among the tested doses of crude glycerin. However, reductions in dry matter and gas losses were observed as a function of crude glycerin doses. Bacterial populations of lactic acid and facultative mesophilic anaerobes also increased as a function of crude glycerin inclusion. Crude glycerin increased ether extract levels and reduced neutral detergent fiber and acid detergent fiber concentrations (dilution effect), improving chemical composition of the silage. Crude glycerin can be used in the ensiling of Piatã grass and the dose of 300 g kg −1 of DM yields the best results.Key Words: biodiesel, byproduct, forage conservation, glycerol, ruminants Revista Brasileira de Zootecnia

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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The use of crude glycerin as an alternative to reduce fermentation losses and enhance the nutritional value of Piatã grass silage

et al. 2017

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“…This species has been isolated by other authors in lactating cows, dry cows and heifers (Lund ; Almeida et al ; Sirisan et al ; Vargas‐Bello‐Pérez et al ) and represents 84·21% of yeasts isolated from the rumen fluid of animals evaluated by Almeida et al (). Pichia kudriavzevii was reported as being the predominant species in corn and sugarcane silages (Santos et al ; Carvalho et al ). The predominance of Pi.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of yeasts from bovine rumen for potential use as probiotics

et al. 2019

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Aims:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Methods and Results: Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain viability in a medium with different ruminal conditions. A second screening in fresh rumen fluid to assess the growth of inoculated yeasts and evaluate in vitro neutral detergent fibre digestibility (NDF-D), pH and acid accumulation was conducted. The yeast population ranged from 3Á84 to 6Á76 log 10 CFU per ml. The main species of yeast found were Pichia kudriavzevii, Candida rugosa, C. pararugosa, C. ethanolica and Magnusiomyces capitatus. Strains CCMA 933 (C. rugosa) and CCMA 970 (C. pararugosa) showed greater ability to survive in ruminal fluid and stimulated the production of acids. Isolate CCMA 967 (C. ethanolica) survived and improved the NDF-D. Conclusion: Pichia kudriavzevii was the dominant yeast found in the cattle herds. Strains CCMA 933, CCMA 970 and CCMA 967 showed properties that could be useful as potential probiotics for cattle. Significance and Impact of the Study: This study was the first to select yeasts from the rumen fluid, with the potential to be used as probiotic, based on the ruminal conditions. the contact of the cell wall with the intestinal lumen triggers an immune response (Dhama et al. 2015).Yeasts also appear to stimulate the growth and activity of fibrolytic ruminal bacteria by providing vitamins and growth factors (Moya et al. 2017). However, contradictory results are reported regarding interactions of diets, animals and environment (Chung et al. 2011). There are reports in the literature that the presence of some species Journal of Applied Microbiology 127, 845--855

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“…In the other corn silage trial (Trial I), LH was probably unable to dominate the fermentation and this could explain the lack of a response in acetic acid production and thus improved aerobic stability. LH has recently been proposed by Carvalho et al . as an inoculum on sugar cane silage to increase aerobic stability.…”

Section: Discussionmentioning

confidence: 99%

Effects of conservation period and Lactobacillus hilgardii inoculum on the fermentation profile and aerobic stability of whole corn and sorghum silages

et al. 2018

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BACKGROUND: Lactic acid bacteria inocula have been developed over the years to improve the aerobic stability of silages. The aims of the study were to evaluate the effect of various conservation periods and the use of Lactobacillus hilgardii inoculum on aerobic stability, fermentative profile and microbial population of corn and sorghum silages. Trials were carried out on two corns and one sorghum crops. The crops were untreated or treated with L. buchneri (LB, application rate 300 000 cfu g −1 FM), L. hilgardii (LH, application rate 300 000 cfu g −1 FM) and a combination (LB + LH, application rate 150 000 cfu g −1 FM each). Silos were opened after 15, 30, 100 and 250 days of conservation, and the silages were analysed for fermentative profile, microbial count and aerobic stability. RESULTS: During conservation, the inocula influenced the fermentation profile. The use of LH increased the aerobic stability at 15 and 30 days in one out of three trials, while after 100 and 250 days, the presence of LB alone or with LH led to greater stability. In all the trials, the acetic acid content increased, the yeast count decreased and the aerobic stability increased during the conservation period. CONCLUSIONS: This study has shown that a long period in complete anaerobiosis reduced yeast count and improved aerobic stability in all silages. The addition of LB was confirmed to be a good option for increasing aerobic stability of silages, whereas the effect of LH alone or in combination with LB on aerobic stability was not consistent between trials. Sample preparation and analysesAll replicates of pre-ensiled material and the silage were split into five subsamples. One subsample was analysed immediately for the DM content by oven drying at 80 ∘ C for 24 h. DM was corrected according to Porter and Murray,25 in order to consider the losses of volatile compounds that can take place at 80 ∘ C. The second subsample was oven-dried at 65 ∘ C to constant weight and was air-equilibrated, weighed and ground in a Cyclotec mill (Tecator, Herndon, VA, USA) to pass a 1 mm screen. The dried samples were analysed for total nitrogen, according to the Dumas method (method number 992.23), 26 using a nitrogen analyser (Primacs SN, Skalar, Breda, The Netherlands), for crude protein (CP) (total N × 6.25), for ash by ignition (method number 942.05), 26 for water-soluble carbohydrates (WSC), by the phenol sulfuric acid method, according to DuBois et al., 27 and for ether extract (EE), J Sci Food Agric 2019; 99: 2530-2540

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Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Cited by 7 publications

References 34 publications

The use of crude glycerin as an alternative to reduce fermentation losses and enhance the nutritional value of Piatã grass silage

The use of crude glycerin as an alternative to reduce fermentation losses and enhance the nutritional value of Piatã grass silage

Identification and characterization of yeasts from bovine rumen for potential use as probiotics

Effects of conservation period and Lactobacillus hilgardii inoculum on the fermentation profile and aerobic stability of whole corn and sorghum silages

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