Methylotrophy in
            <i>Methylobacterium extorquens</i>
            AM1 from a Genomic Point of View

Chistoserdova, Ludmila; Chen, Sung–Wei; Lapidus, Alla; Lidstrom, Mary E.

doi:10.1128/jb.185.10.2980-2987.2003

Cited by 268 publications

(277 citation statements)

References 55 publications

Supporting

Mentioning

275

Contrasting

Order By: Relevance

“…Methylobacterium spp. are specialists in dealing with toxic compounds, as is already clear when considering that formaldehyde is a central intermediate of methylotrophic metabolism (10,15). This question of detoxification vs. catabolism also arises for a putative lactoylglutathione lyase (GloA) ( Table 1).…”

mentioning

confidence: 99%

“…Methylobacterium spp. on plant surfaces benefit from methanol produced by plants (14) by means of methylotrophy (10,15). However, methanol is not the only carbon substrate that these bacteria are able to consume in the phyllosphere (14).…”

mentioning

confidence: 99%

“…For this work, we have used the Alphaproteobacterium Methylobacterium extorquens AM1, a well studied model pinkpigmented facultative methylotroph (PPFM) (10), whose draft genomesequenceisavailable(seewww.integratedgenomics.com͞ genomereleases.html#6). Methylobacterium spp.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth

Gourion

Rossignol

Vorholt

2006

Proc. Natl. Acad. Sci. U.S.A.

151

129

View full text Add to dashboard Cite

Aerial plant surfaces are colonized by diverse bacteria such as the ubiquitous Methylobacterium spp. The specific physiological traits as well as the underlying regulatory mechanisms for bacterial plant colonization are largely unknown. The purpose of this study was to identify proteins produced specifically in the phyllosphere by comparing the proteome of Methylobacterium extorquens colonizing the leaves either with that of bacteria colonizing the roots or with that of bacteria growing on synthetic medium. We identified 45 proteins that were more abundant in M. extorquens present on plant surfaces as compared with bacteria growing on synthetic medium, including 9 proteins that were more abundant on leaves compared with roots. Among the proteins induced during epiphytic growth, we found enzymes involved in methanol utilization, prominent stress proteins, and proteins of unknown function. In addition, we detected a previously undescribed type of two-domain response regulator, named PhyR, that consists of an N-terminal sigma factor (RpoE)-like domain and a C-terminal receiver domain and is predicted to be present in essentially all Alphaproteobacteria. The importance of PhyR was demonstrated through phenotypic tests of a deletion mutant strain shown to be deficient in plant colonization. Among PhyR-regulated gene products, we found a number of general stress proteins and, in particular, proteins known to be involved in the oxidative stress response such as KatE, SodA, AhpC, Ohr, Trx, and Dps. The PhyRregulated gene products partially overlap with the bacterial in planta-induced proteome, suggesting that PhyR is a key regulator for adaptation to epiphytic life of M. extorquens.fitness ͉ sigma factor ͉ stress ͉ two-component system

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth

Gourion

Rossignol

Vorholt

2006

Proc. Natl. Acad. Sci. U.S.A.

151

129

View full text Add to dashboard Cite

show abstract

“…MDH of Methylobacillus glycogens showed an α 2 β 2 confi guration as well (Adachi et al, 1990). Genes mxaF and mxaI encode the large (α) and small (β) subunits of MDH (Chistoserdova et al, 2003;Lidstrom, 1992;Lidstrom et al, 1994). The mxaF gene has been described to contain the active site of the enzyme and to be highly conserved in the methylotrophs and methanotrophs of α-Proteobacteria (McDonald and Murrell, 1997).…”

Section: Introductionmentioning

confidence: 99%

MxaF gene, a gene encoding alpha subunit of methanol dehydrogenase in and false growth of acetic acid bacteria on methanol

Suzuki

Lisdiyanti

Komagata

et al. 2009

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

MxaF gene, a gene encoding alpha subunit of methanol dehydrogenase, was investigated for acetic acid bacteria, and growth on methanol was examined for the bacteria by using various media. Of 21 strains of acetic acid bacteria studied, Acidomonas methanolica strains showed the presence of mxaF gene exclusively, and grew on a defi ned medium containing methanol. Further, none of the strains tested of which the growth on methanol had been previously reported, except for Acidomonas methanolica, showed the presence of mxaF gene or the growth on methanol. Precautions were taken against false growth on compounds used for identifi cation of bacteria.

show abstract

“…It can also grow on multi-carbon compounds such as pyruvate and succinate. M. extorquens AM1 is one of the most intensively studied methylotrophs (Chistoserdova et al, 2003) and recently a gapped genome sequence has been made available for this organism (http:// www.integratedgenomics.com/genomereleases.html#list6). Methylotrophic metabolism in M. extorquens AM1 begins with the oxidation of methanol or methylamine to formaldehyde in the periplasm (Anthony, 1982).…”

Section: Introductionmentioning

confidence: 99%

Identification of an upstream regulatory sequence that mediates the transcription of mox genes in Methylobacterium extorquens AM1

2005

Self Cite

View full text Add to dashboard Cite

A multiple A-tract sequence has been identified in the promoter regions for the mxaF, pqqA, mxaW, mxbD and mxcQ genes involved in methanol oxidation in Methylobacterium extorquens AM1, a facultative methylotroph. Site-directed mutagenesis was exploited to delete or change this conserved sequence. Promoter-xylE transcriptional fusions were used to assess promoter activity in these mutants. A fiftyfold drop in the XylE activity was observed for the mxaF and pqqA promoters without this sequence, and a five-to sixfold drop in the XylE activity was observed for the mxbD and mxcQ promoters without this sequence. Mutants were generated in the chromosomal copies in which this sequence was either deleted or altered, and these mutants were unable to grow on methanol. When one of these sequences was added to Plac of Escherichia coli, which is a weak constitutive promoter in M. extorquens AM1, the activity increased two-to threefold. These results suggest that this sequence is essential for normal expression of these genes in M. extorquens AM1, and may serve as a general enhancer element for genetic constructs in this bacterium. INTRODUCTIONMethylobacterium extorquens AM1 (Peel & Quayle, 1961) is a facultative methylotroph that is able to use C 1 compounds as its sole carbon and energy source (Anthony, 1982(Anthony, , 2000Lidstrom, 1991). It can also grow on multi-carbon compounds such as pyruvate and succinate. M. extorquens AM1 is one of the most intensively studied methylotrophs (Chistoserdova et al., 2003) and recently a gapped genome sequence has been made available for this organism (http:// www.integratedgenomics.com/genomereleases.html#list6). Methylotrophic metabolism in M. extorquens AM1 begins with the oxidation of methanol or methylamine to formaldehyde in the periplasm (Anthony, 1982). Further assimilation and dissimilation of formaldehyde occur in the cytoplasm (Marx et al., 2003). Methanol oxidation is carried out by the enzyme methanol dehydrogenase, which is a quinoprotein using pyrroloquinoline quinone (PQQ) as a prosthetic group, and is an a 2 b 2 heterotetramer coupled to a specific cytochrome c accepter (Anthony, 1982). Methanol dehydrogenase activity and proteins have been shown to be regulated by carbon source in M. extorquens AM1, with three-to sixfold higher levels in the presence of methanol than during growth on multicarbon substrates in the absence of C 1 compounds (Nunn & Lidstrom, 1986a, b).At least 25 genes have been identified to be involved in the methanol oxidation reaction in M. extorquens AM1 (Lidstrom, 1991;Zhang & Lidstrom, 2003). These Mox genes are distributed between three different loci: mxa, mxb and mxc. Fourteen genes (mxaFJGIRSACKLDEHB) transcribed in the same direction, together with an additional gene (mxaW) transcribed in the opposite direction, are located on the mxa gene cluster (Anderson et al., 1990;Morris et al., 1995;Springer et al., 1998Springer et al., , 1995. The large and small subunits of methanol dehydrogenase are encoded by mxaF and mxaI, respectively (Anderson & ...

show abstract

Methylotrophy in Methylobacterium extorquens AM1 from a Genomic Point of View

Cited by 268 publications

References 55 publications

A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth

A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth

MxaF gene, a gene encoding alpha subunit of methanol dehydrogenase in and false growth of acetic acid bacteria on methanol

Identification of an upstream regulatory sequence that mediates the transcription of mox genes in Methylobacterium extorquens AM1

Contact Info

Product

Resources

About