Metodología clásica y molecular en la identificación de especies de Enterococcus spp

A, Marcela Sepúlveda; T, Helia Bello; C, Mery Ruiz; F, José Hormazábal; Y, Mariana Domínguez; R, Gerardo González; M, Sergio Mella; Z, Raúl Zemelman

doi:10.4067/s0034-98872002000100006

Search citation statements

Order By: Relevance

Paper Sections

Select...

Materials Y Método1

Citation Types

Supporting

Mentioning

Contrasting

Unclassified

Year Published

2007

2015

Publication Types

Select...

Article2

Relationship

Self Cite1

Independent1

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Se incluyeron 305 cepas de Enterococcus spp aisladas en hospitales de la VIII Región entre los años 1996 y 1999. La identificación de éstas se realizó de acuerdo al protocolo estandarizado por nuestro grupo 10 .…”

Section: Materials Y Métodounclassified

Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile

A¹,

T²,

Y³

et al. 2007

Rev. méd. Chile

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Materials Y Métodounclassified

Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile

A¹,

T²,

Y³

et al. 2007

Rev. méd. Chile

Self Cite

View full text Add to dashboard Cite

show abstract

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

Sánchez‐Sanhueza¹,

González-Rocha²,

Dominguez³

et al. 2015

Braz. J. Oral Sci.

View full text Add to dashboard Cite

Aim: To isolate and identify in a Chilean population, Enterococcus spp. from root canals with persistent chronic apical periodontitis (CAP) and to investigate the potential correlation between the bacteria and the observed clinical features. Methods: Twenty patients with indication for endodontic retreatment due to persistent CAP were selected. Data from patient general health and dental clinical history were recorded. During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. The relationship between the number of colony-forming units of Enterococcus spp. and patient clinical data was assessed statistically by the Pearson Chi square and Fisher exact tests. Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. was conducted in the clinical samples, and the results were analyzed by a proportion comparison test. Results: Enterococcus spp. strains were isolated in 70% of the patients. Most of them (98.8%) accounted for Enterococcus faecalis and only 1.2% for Enterococcus faecium. A high frequency of E. faecalis was found in teeth with inadequate endodontic treatment or dental crown restorations. Conclusions: This study concluded that E. faecalis is prevalent in root canals with persistent CAP in a Chilean population. E. faecium as found in a single case with the poorest root canal filling. Further studies are still required to investigate the presence of other species, which may be linked to persistent chronic apical periodontitis.

show abstract

Metodología clásica y molecular en la identificación de especies de Enterococcus spp

Cited by 2 publications

References 14 publications

Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile

Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

Contact Info

Product

Resources

About