Métodos moleculares para la determinación del genotipo del virus de la hepatitis C

Ortega, David Navarro; Mayordomo, Marta Jiménez; Aparicio, M. Desamparados Martínez

doi:10.1157/13111840

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…A variety of technologies have been developed for HCV GT and subtype determination 10–18 . So far, several non-sequencing-based HCV genotyping assays, mainly based on reverse hybridization or real-time PCR (RT-PCR), such as the Abbott RealTime HCV Genotype II assay (Abbott RT-PCR), are commercially available and used for routine determination of the HCV GT and selected subtypes.…”

Section: Introductionmentioning

confidence: 99%

Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing

Fernández-Caso

Fernández-Caballero

Chueca

et al. 2019

Sci Rep

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Current HCV genotyping methods may have some limitations in detecting mixed infections. We aimed to determine the accuracy of genotyping and the detection of mixed-genotype infections using the Abbott-RealTime HCV Genotype II assay (Abbott-RT-PCR) in comparison with a Roche-Next Generation Sequencing assay (Roche-NGS). Plasma samples collected from 139 HCV-infected patients tested with Abbott-RT-PCR, 114 with single genotype (GT) and 25 with mixed GTs were genotyped using Roche-NGS. Roche-NGS confirmed all single GTs obtained with Abbott-RT-PCR. One case of Abbott GT 4 was found as GT 1a using Roche-NGS. Genotype 5 was confirmed using Roche-NGS in 75% cases (3 out of 4 cases). Twenty-five patients were identified as having mixed HCVinfections using Abbott-RT-PCR. The concordance between Abbott-RT-PCR and Roche-NGS was 76% (19 out of 25 cases). Three mixed-GT infections identified with the Abbott assay (two (1b + 4); one (1a + 3)) were reported as pure 1b using Roche-NGS. Very divergent results were found for the other three samples. When compared to Roche-NGS, Abbott-RT-PCR has performed excellently for the determination of patients infected with single GTs. For patients that are categorized as having a mixed infection using Abbott-RT-PCR, we recommend an NGS assay as a confirmation test.

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Section: Introductionmentioning

confidence: 99%

Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing

Fernández-Caso

Fernández-Caballero

Chueca

et al. 2019

Sci Rep

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Incidence of viral hepatitis in Brazil from 2009 to 2018: an epidemiological study of confirmed cases of viral hepatitis

Silva

Souza

et al. 2021

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Viral hepatitis is a major public health problem. It is necessary to understand the epidemic, verifying the combination of biological and demographic characteristics. METHODS: This is an analytical ecological and epidemiological study. Confirmed case data from the Notification Disease Information System (SINAN) were used. RESULTS: From 2009-2018, SINAN confirmed 404,003 viral hepatitis cases in Brazil, with 12.49%, 37.06%, and 48.28% cases of hepatitis A, B, and C, respectively. CONCLUSIONS: In Brazil, 4,296 deaths were associated with viral hepatitis, of which 36.66% were associated with acute hepatitis B. The proportional distribution of cases varied among the five Brazilian regions.

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Métodos moleculares para la determinación del genotipo del virus de la hepatitis C

Cited by 2 publications

References 45 publications

Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing

Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing

Incidence of viral hepatitis in Brazil from 2009 to 2018: an epidemiological study of confirmed cases of viral hepatitis

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