Metronidazole Susceptibility and TVV-infection of Trichomonas vaginalis from Metro Manila and Angeles City, Philippines

Abstract

Background. Metronidazole susceptibility and the presence of Trichomonas vaginalis virus (TVV) are the phenotypes found to be significantly correlated with the microsatellite-based genotypes of T. vaginalis. These phenotypes were assessed in T. vaginalis isolates from select urban areas to determine preliminary “type” of Philippine T. vaginalis.
 Methods. Culture and microscopy were used to detect T. vaginalis in vaginal swab samples collected from women attending social hygiene clinics in Metro Manila and Angeles City, Philippines. Screening of TVV on T. vaginalis was performed using RNA gel electrophoresis and RT-PCR. A modified protocol for metronidazole susceptibility assay was used to determine the aerobic minimum lethal concentration (MLC) of metronidazole in axenized T. vaginalis isolates.
 Results. A total of 42 T. vaginalis were screened for the presence of TVV and assayed for metronidazole susceptibility. TVV was detected in 13 of the isolates. All but one of the samples was susceptible to metronidazole.
 Conclusion. This is the first study to assess the in vitro metronidazole susceptibility of Philippine T. vaginalis isolates. The isolates are generally susceptible to metronidazole even with the presence of TVV. The metronidazole susceptibility and presence of TVV are not enough to classify the isolates into type 1 or type 2.