MeV-SIMS TOF Imaging of Organic Tissue with Continuous Primary Beam

Jenčič, Boštjan; Vavpetič, Primož; Kelemen, Mitja; Vencelj, M.; Vogel‐Mikuš, Katarina; Kavčič, Anja; Pelicon, Primož

doi:10.1007/s13361-019-02258-8

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…If the thickness of an analysed sample is less than 10 μ m, then an alternative aproach with a continuous primary ion beam can improve the beam resolution to sub micrometre level. However, such approach is not possible with human hair samples [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

“…The technique features a high potential lateral resolution; as no sample-surface pre-processing is required and the damage cross-section associated with the impact of an individual primary ion is in the order of few square nanometers [ 42 ], the lateral resolution of MeV SIMS is primarily determined by the size of the primary ion beam. Submicrometer lateral resolution studies with MeV-SIMS were recently reported [ 43 , 44 ]. Our results show that MeV-SIMS allows determining cocaine concectration in human hair with a lateral resolution down to 50 μ m without the need for a chemical preparation of the sample.…”

Section: Introductionmentioning

confidence: 99%

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Molecular imaging of humain hair with MeV-SIMS: A case study of cocaine detection and distribution in the hair of a cocaine user

et al. 2022

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Human hair absorbs numerous biomolecules from the body during its growth. This can act as a fingerprint to determine substance intake of an individual, which can be useful in forensic studies. The cocaine concentration profile along the growth axis of hair indicates the time evolution of the metabolic incorporation of cocaine usage. It could be either assessed by chemical extraction and further analysis of hair bundels, or by direct single hair fibre analysis with mass spectroscopy imaging (MSI). Within this work, we analyzed the cocaine distribution in individual hair samples using MeV-SIMS. Unlike conventional surface analysis methods, we demonstrate high yields of nonfragmented molecular ions from the surface of biological materials, resulting in high chemical sensitivity and non-destructive characterisation. Hair samples were prepared by longitudinally cutting along the axis of growth, leaving half-cylindrical shape to access the interior structure of the hair by the probing ion beam, and attached to the silicon wafer. A focused 5.8 MeV 35Cl6+ beam was scanned across the intact, chemically pristine hair structure. A non-fragmented protonated [M+ H]+ cocaine molecular peak at m/z = 304 was detected and localized along the cross-section of the hair. Its intensity exhibits strong fluctuations along the direction of the hair’s growth, with pronounced peaks as narrow as 50 micrometres, corresponding to a metabolic incorporation time of approx. three hours.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular imaging of humain hair with MeV-SIMS: A case study of cocaine detection and distribution in the hair of a cocaine user

et al. 2022

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“…With the application of UHPLC-ESI- MS/MS, it is possible to analyze in buckwheat a broad list of metabolites, such as three different phenolic acids, four flavanols, four flavones, seven flavonols, and two flavanones [ 23 , 24 ]). The multimodal bioimaging of Tartary buckwheat for revealing the allocation of metabolites in different parts of Tartary buckwheat plants is under development [ 22 , 25 , 26 , 27 ]. Ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry multiple reaction monitoring (UPLC-MS/MS MRM) seems to be suitable for determining fagopyrins in Tartary buckwheat extracts as suggested [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemistry, Bioactivities of Metabolites, and Traditional Uses of Fagopyrum tataricum

et al. 2022

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In Tartary buckwheat (Fagopyrum tataricum), the edible parts are mainly grain and sprouts. Tartary buckwheat contains protecting substances, which make it possible for plants to survive on high altitudes and under strong natural ultraviolet radiation. The diversity and high content of phenolic substances are important for Tartary buckwheat to grow and reproduce under unfriendly environmental effects, diseases, and grazing. These substances are mainly flavonoids (rutin, quercetin, quercitrin, vitexin, catechin, epicatechin and epicatechin gallate), phenolic acids, fagopyrins, and emodin. Synthesis of protecting substances depends on genetic layout and on the environmental conditions, mainly UV radiation and temperature. Flavonoids and their glycosides are among Tartary buckwheat plants bioactive metabolites. Flavonoids are compounds of special interest due to their antioxidant properties and potential in preventing tiredness, diabetes mellitus, oxidative stress, and neurodegenerative disorders such as Parkinson’s disease. During the processing and production of food items, Tartary buckwheat metabolites are subjected to molecular transformations. The main Tartary buckwheat traditional food products are bread, groats, and sprouts.

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“…Secondary Ion Mass Spectrometry using MeV ions (MeV SIMS) 1 is a fairly new Ion Beam Analysis (IBA) technique that is being increasingly used for the analysis and imaging of organic materials in various fields, such as forensics (fingerprints 2 and inks 3 – 5 ), cultural heritage (paints 6 ), biology (plants 7 and tissues 8 ), etc. Conventional keV SIMS, on the other hand, is a well-established technique used mainly in the analysis and depth profiling of inorganic materials, with the most popular application in the semiconductor industry, i.e.…”

Section: Introductionmentioning

confidence: 99%

Depth profiling of Cr-ITO dual-layer sample with secondary ion mass spectrometry using MeV ions in the low energy region

Barac

Brajković

Siketić

et al. 2022

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This work explores the possibility of depth profiling of inorganic materials with Megaelectron Volt Secondary Ion Mass Spectrometry using low energy primary ions (LE MeV SIMS), specifically 555 keV Cu2+, while etching the surface with 1 keV Ar+ ions. This is demonstrated on a dual-layer sample consisting of 50 nm Cr layer deposited on 150 nm In2O5Sn (ITO) glass. These materials proved to have sufficient secondary ion yield in previous studies using copper ions with energies of several hundred keV. LE MeV SIMS and keV SIMS depth profiles of Cr-ITO dual-layer are compared and corroborated by atomic force microscopy (AFM) and time-of-flight elastic recoil detection analysis (TOF-ERDA). The results show the potential of LE MeV SIMS depth profiling of inorganic multilayer systems in accelerator facilities equipped with MeV SIMS setup and a fairly simple sputtering source.

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MeV-SIMS TOF Imaging of Organic Tissue with Continuous Primary Beam

Cited by 10 publications

References 32 publications

Molecular imaging of humain hair with MeV-SIMS: A case study of cocaine detection and distribution in the hair of a cocaine user

Molecular imaging of humain hair with MeV-SIMS: A case study of cocaine detection and distribution in the hair of a cocaine user

Phytochemistry, Bioactivities of Metabolites, and Traditional Uses of Fagopyrum tataricum

Depth profiling of Cr-ITO dual-layer sample with secondary ion mass spectrometry using MeV ions in the low energy region

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