Mexican Strains of<i>Hirsutella</i>Isolated from<i>Diaphorina citri</i>(Hemiptera: Liviidae): Morphologic and Molecular Characterization

Pérez-González, Orquídea; Rodríguez-Villarreal, Raúl Asael; López-Arroyo, J. Isabel; Maldonado-Blanco, María Guadalupe; Rodríguez-Guerra, Raúl

doi:10.1653/024.098.0147

Cited by 15 publications

(10 citation statements)

References 26 publications

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“… Subandiyah et al . 2000 , Hall et al 2012 , Pérez-González et al 2015 ) and is represented in our phylogeny by isolates collected in the Republic of Indonesia, Republic of the Philippines, and the USA. This species is monophyletic, indicating reliable morphological identification by different investigators, but Pérez-González et al (2015) argued that morphologies of phialides and conidia of isolates vary from those of the original description and warn that new isolates could show increasing variability in these structures.…”

Section: Discussionmentioning

confidence: 99%

Phylogeny of Hirsutella species (Ophiocordycipitaceae) from the USA: remedying the paucity of Hirsutella sequence data

Simmons

et al. 2015

IMA Fungus

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Hirsutella (Ophiocordycipitaceae: Hypocreales) is a genus of insect, mite, and nematode pathogens with an asexual morph, which generally produce a mucilaginous cluster of one or several conidia on phialides that are basally subulate and taper to a fine neck. The generic name Hirsutella has been proposed for suppression in favour of Ophiocordyceps as a consequence of the ending of dual nomenclature for different morphs of pleomorphic fungi in 2011. Though the generic name is well established, geographically dispersed, and speciose, exceptionally few sequences are available in online databases. We examined 46 isolates of 23 Hirsutella species from the USA, curated by the USDA-ARS Collection of Entomopathogenic Fungal Cultures (ARSEF Culture Collection), that previously had not been molecularly characterized and produced a phylogeny of these organisms; we included previously published Hirsutella and Ophiocordyceps taxa. In producing the largest phylogeny of Hirsutella isolates so far, we provide: (1) context for discussing previously-hypothesized relationships; (2) evidence for revisions as taxonomic transitions move forward; and (3) available molecular data to be incorporated into further evolutionary studies of Ophiocordycipitaceae.

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Section: Discussionmentioning

confidence: 99%

Phylogeny of Hirsutella species (Ophiocordycipitaceae) from the USA: remedying the paucity of Hirsutella sequence data

Simmons

et al. 2015

IMA Fungus

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“…In this regard, H. citriformis reproductive structure measurements directly obtained from the host [ 7 , 16 , 17 ] or reproductive structures developed in culture medium [ 6 , 12 , 18 ] are different from previous reports on these species [ 10 ] ( Figure 2 ). In host-derived species, isolates are found in different hemipteran species and geographic regions, which may result in diverse fungal structures development.…”

Section: Hirsutella Citriformis Identificationmentioning

confidence: 71%

“…In Mexico, H. citriformis has been isolated from D. citri Kuwayama (Hemiptera: Liviidae) carcasses, whose first isolate was reported in Veracruz state in 2008, after which it has been detected in different locations, mainly in Mexican citrus areas [ 12 , 18 , 25 , 27 , 28 ] ( Table 4 ).…”

Section: Insects Associated With H Citriformismentioning

confidence: 99%

Insight into Biological Control Potential of Hirsutella citriformis against Asian Citrus Psyllid as a Vector of Citrus Huanglongbing Disease in America

Pérez-González

Gómez-Flores

Tamez‐Guerra

2022

JoF

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Studies on Hirsutella citriformis Speare are scarce. Among these, some reports have focused on phenotypic identification, based on its morphological structure and morphometric characteristics. This fungus is known to control economically important citrus crop pests. In recent years, H. citriformis has received increased attention as a control agent for the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), which causes the Huanglongbing (HLB) disease. Unfortunately, formal H. citriformis strains characterization is marginal, which mainly involves the role of biologically active exudates (metabolites) produced during their growth. Information regarding their mode of action and biocontrol potential is limited. However, epizootics reports of this fungus, under suitable environmental conditions for its development (25 °C to 28 °C and ~80% relative humidity), have demonstrated its parasitization efficacy. Therefore, it becomes challenging to determine whether H. citriformis strains may be developed as commercial products. In this review, we showed relevant information on isolation and bioassay strategies of H. citriformis to evaluate potential biocontrol strains under laboratory and field conditions in America.

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“…En México, se ha reportado a H. citriformis infectando a D. citri en la mayoría de las zonas citrícolas que incluyen Chiapas, Quintana Roo, San Luis Potosí, Tabasco, Veracruz, Yucatán (Pérez-González et al, 2015), Nuevo León y Tamaulipas (Casique-Valdes et al, 2011). Hirsutella citriformis se encontró en todos los huertos muestreados, por lo que fue el hongo con mayor incidencia y distribución en Colima.…”

Section: Discussionunclassified

Hongos entomopatógenos asociados a Diaphorina citri (Hemiptera: Liviidae) en Colima, México

Berlanga-Padilla¹,

Gallou²,

Ayala-Zermeño³

et al. 2018

Rev.Mex.Biodiv.

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Resumen Diaphorina citri es el vector del Huanglongbing (HLB), enfermedad devastadora de los cítricos a escala mundial. Se realizó la búsqueda de hongos entomopatógenos (HE) como agentes potenciales de control biológico de D. citri en huertas comerciales de cítricos y plantas de mirto en zonas urbanas del estado de Colima, México. Los hongos que se encontraron asociados a la plaga fueron aislados e identificados por métodos morfológicos y moleculares. Se aislaron 30 cepas de HE del orden Hypocreales: Hirsutella citriformis, Isaria javanica, Beauveria bassiana y Simplicillium lanosoniveum; 2 géneros de HE del orden Entomophthorales (Batkoa sp. y Entomophthora sp.) de los cuales no se logró su aislamiento. Se reporta por primera ocasión, la incidencia natural de Simplicillium lanosoniveum y Batkoa sp. asociados a D. citri en Colima, México. Por la incidencia con la que se encontró a H. citriformis, podría considerarse como un buen candidato para el control biológico del psílido D. citri, que al igual que las especies de Entomophthorales, abren la posibilidad de explorar su potencial como agentes microbianos de este psílido.

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Mexican Strains ofHirsutellaIsolated fromDiaphorina citri(Hemiptera: Liviidae): Morphologic and Molecular Characterization

Cited by 15 publications

References 26 publications

Phylogeny of Hirsutella species (Ophiocordycipitaceae) from the USA: remedying the paucity of Hirsutella sequence data

Phylogeny of Hirsutella species (Ophiocordycipitaceae) from the USA: remedying the paucity of Hirsutella sequence data

Insight into Biological Control Potential of Hirsutella citriformis against Asian Citrus Psyllid as a Vector of Citrus Huanglongbing Disease in America

Hongos entomopatógenos asociados a Diaphorina citri (Hemiptera: Liviidae) en Colima, México

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