Mfc1 Is a Novel Forespore Membrane Copper Transporter in Meiotic and Sporulating Cells

Beaudoin, Jude; Ioannoni, Raphaël; López‐Maury, Luis; Bähler, Jürg; Aı̈t-Mohand, Samia; Guérin, Brigitte; Dodani, Sheel C.; Chang, Christopher J.; Labbé, Simon

doi:10.1074/jbc.m111.280396

Cited by 37 publications

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“…It has recently been reported that S. pombe undergoes meiotic arrest at metaphase I under strong copper starvation conditions, suggesting a critical role for copper in meiotic maturation and progression (3). During early meiosis, copper uptake is most likely ensured by the heteromeric Ctr4-Ctr5 protein complex, since Ctr4 localizes to the cell surface of developing zygotic cells (3). Once middle-phase meiosis has been initiated, Ctr4 expression is abolished and Mfc1, a meiosis-specific major facilitator superfamily (MFS)-type transporter, is induced.…”

Section: Discussionmentioning

confidence: 99%

“…During middle to late meiosis, Mfc1 localizes at the forespore membrane, where it potentially mediates copper uptake into the forespore. Studies of the full-scale meiotic transcriptional program have revealed that ctr4 ϩ and mfc1 ϩ transcript levels are induced at distinct times after meiotic induction, in response to copper starvation (3). Whereas deletion of the gene encoding the copper-sensing transcription factor Cuf1 (cuf1⌬) impairs the induction of ctr4 ϩ and ctr5 ϩ , the activation of mfc1 ϩ is unaffected, suggesting the existence of a distinct transcriptional regulator for the induction of mfc1 ϩ in response to copper starvation (3).…”

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confidence: 99%

“…Recent studies have shown that metal ions such as copper and zinc are required for normal progression of meiosis in S. pombe and mice, respectively (3,4,15). In S. pombe, copper-insufficient zygotes undergo a meiotic block at metaphase I (3).…”

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confidence: 99%

“…Once MI and MII divisions have been completed, a differentiation process is induced to produce four gametes ready for fertilization or germination. Recent studies have shown that micronutrients such as copper and zinc are required for normal progression of meiosis because insufficient amounts of these metal ions lead to meiotic blocks and defective gamete formation (3,4).…”

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confidence: 99%

“…This step is followed by a quick increase in mfc1 ϩ mRNA levels at the 3-h meiotic time point, with mfc1 ϩ transcript levels remaining sustained throughout the meiotic program. mfc1 ϩ encodes a major facilitator superfamily (MFS)-type copper transporter (3,20). During middle to late meiosis, Mfc1 localizes at the forespore membrane, where it potentially mediates copper uptake into the forespore.…”

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Transcriptional Regulation of the Copper Transporter Mfc1 in Meiotic Cells

et al. 2013

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Mfc1 is a meiosis-specific protein that mediates copper transport during the meiotic program in Schizosaccharomyces pombe. Although the mfc1؉ gene is induced at the transcriptional level in response to copper deprivation, the molecular determinants that are required for its copper starvation-dependent induction are unknown. Promoter deletion and site-directed mutagenesis have allowed identification of a new cis-regulatory element in the promoter region of the mfc1 ؉ gene. This cis-acting regulatory sequence containing the sequence TCGGCG is responsible for transcriptional activation of mfc1 ؉ under low-copper conditions. The TCGGCG sequence contains a CGG triplet known to serve as a binding site for members of the Zn (2) Cys (

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Transcriptional Regulation of the Copper Transporter Mfc1 in Meiotic Cells

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Copper Transport in Fungi

Labbé

Beaudoin

Ioannoni

2004

Encyclopedia of Inorganic and Bioinorganic Chemistry

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A clear understanding of copper metabolism is critical because copper is absolutely essential but also toxic. Significant new knowledge in this field was achieved through research using yeast models, including Saccharomyces cerevisiae and Schizosaccharomyces pombe . Both yeasts are highly needed model organisms for identifying the molecular mechanisms whereby cells appropriately acquire copper and regulate gene expression in response to changes in copper levels. This article summarizes our current understanding of a number of components involved in copper uptake, trafficking, compartmentalization, and copper sensing to establish and maintain normal copper homeostasis.

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Thioether Coordination Chemistry for Molecular Imaging of Copper in Biological Systems

Ramos-Torres

Kölemen

Chang

2016

Israel Journal of Chemistry

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Copper is an essential element in biological systems. Its potent redox activity renders it necessary for life, but at the same time, misregulation of its cellular pools can lead to oxidative stress implicated in aging and various disease states. Copper is commonly thought of as a static cofactor buried in protein active sites; however, evidence of a more loosely bound, labile pool of copper has emerged. To help identify and understand new roles for dynamic copper pools in biology, we have developed selective molecular imaging agents for this metal, drawing inspiration from both biological binding motifs and synthetic model complexes that reveal thioether coordination as a general design strategy for selective and sensitive copper recognition. In this review, we summarize some contributions, primarily from our own laboratory, on fluorescence- and magnetic resonance-based molecular-imaging probes for studying copper in living systems using thioether coordination chemistry.

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Mfc1 Is a Novel Forespore Membrane Copper Transporter in Meiotic and Sporulating Cells

Cited by 37 publications

References 64 publications

Transcriptional Regulation of the Copper Transporter Mfc1 in Meiotic Cells

Transcriptional Regulation of the Copper Transporter Mfc1 in Meiotic Cells

Copper Transport in Fungi

Thioether Coordination Chemistry for Molecular Imaging of Copper in Biological Systems

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