MFO Induction of three Australian fish species

Smith, Brent James; Gagnon, Marthe Monique

doi:10.1002/(sici)1522-7278(2000)15:1<1::aid-tox1>3.0.co;2-m

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…EROD activity was assessed by the fluorimetric method described in Smith and Gagnon [ 7 ]. Briefly, livers were homogenized with a Diax 900 ultrasonic homogeniser and centrifuged at 12 000 g for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…One such commonly used biomarker is the hepatic microsomal ethoxyresorufin- O- deethylase (EROD) activity, which has been demonstrated to be a reliable indicator of exposure of fish to certain classes of organic pollutants. EROD activity is known to be induced by a range of pollutants including polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo- p -dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) [ 6 ] and polychlorinated biphenyls (PCBs) [ 7 ], all of which having been identified in the sediments of the Bay in the past [ 1 ]. Furthermore, previous studies measuring hepatic EROD activity in Port Phillip Bay sand flathead also indicated elevated EROD activity where fish were captured in the vicinity of high industrialisation/urbanisation [ 4 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Health Status of Sand Flathead (Platycephalus bassensis), Inhabiting an Industrialised and Urbanised Embayment, Port Phillip Bay, Victoria as Measured by Biomarkers of Exposure and Effects

et al. 2016

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Port Phillip Bay, Australia, is a large semi-closed bay with over four million people living in its catchment basin. The Bay receives waters from the Yarra River which drains the city of Melbourne, as well as receiving the discharges of sewage treatment plants and petrochemical and agricultural chemicals. A 1999 study demonstrated that fish inhabiting Port Phillip Bay showed signs of effects related to pollutant exposure despite pollution management practices having been implemented for over a decade. To assess the current health status of the fish inhabiting the Bay, a follow up survey was conducted in 2015. A suite of biomarkers of exposure and effects were measured to determine the health status of Port Phillip Bay sand flathead (Platycephalus bassensis), namely ethoxyresorufin-O-deethylase (EROD) activity, polycyclic aromatic hydrocarbons (PAH) biliary metabolites, carboxylesterase activity (CbE) and DNA damage (8-oxo-dG). The reduction in EROD activity in the present study suggests a decline in the presence of EROD activity-inducing chemicals within the Bay since the 1990s. Fish collected in the most industrialised/urbanised sites did not display higher PAH metabolite levels than those in less developed areas of the Bay. Ratios of PAH biliary metabolite types were used to indicate PAH contaminant origin. Ratios indicated fish collected at Corio Bay and Hobsons Bay were subjected to increased low molecular weight hydrocarbons of petrogenic origin, likely attributed to the close proximity of these sites to oil refineries, compared to PAH biliary metabolites in fish from Geelong Arm and Mordialloc. Quantification of DNA damage indicated a localised effect of exposure to pollutants, with a 10-fold higher DNA damage level in fish sampled from the industrial site of Corio Bay relative to the less developed site of Sorrento. Overall, integration of biomarkers by multivariate analysis indicated that the health of fish collected in industrialised areas was compromised, with biologically significant biomarkers of effects (LSI, CF and DNA damage) discriminating between individuals collected in industrialised areas from observations made in fish collected in less developed areas of the Bay.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Health Status of Sand Flathead (Platycephalus bassensis), Inhabiting an Industrialised and Urbanised Embayment, Port Phillip Bay, Victoria as Measured by Biomarkers of Exposure and Effects

et al. 2016

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“…Tugiyono and Gagnon (2001) found only a 4-fold increase in EROD activity in pink snapper (Pagrus auratus), a marine species, when injected with PCB-126 at 10 and 100 g per kg of fish. A study in Port Phillip Bay, Victoria, Australia found an 20-fold increase in induction response in sand flathead (Platycephalus bassensis) relative to controls (Smith and Gagnon, 2000).…”

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confidence: 99%

MFO induction potential of fish species native to the Swan–Canning Estuary, Western Australia

Webb

Gagnon

2002

Environmental Toxicology

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The use of mixed function oxygenase (MFO) enzymes as a potential biomarker of pollution in the Swan-Canning Estuary was investigated in four indigenous fish species, Perth herring (Nematalosa vlaminghi), sea mullet (Mugil cephalus), yellowtail trumpeter (Amniataba caudavittata), and black bream (Acanthopagrus butcheri). Each experimental fish was intraperitoneally injected with 10 micrograms/kg of 3,3',4,4',5-pentachlorobiphenyl per kilogram of fish using corn oil as a carrier. Controls were injected with 1 mL corn oil per kilo. Induction of the hepatic ethoxyresorufin-O-deethylase (EROD) activity was measured 10 days post injection. Perth herring did not survive the experimental period due to the poor ability of this fish to endure captivity and handling. Yellowtail trumpeter demonstrated a low EROD induction response (2-fold relative to controls), while sea mullet displayed a moderate EROD induction potential (3.5-fold relative to controls). Because of their lower EROD induction potential, yellowtail trumpeter and sea mullet were not considered the most suitable bioindicator species for Western Australian estuarine waters. Black bream has desirable population traits and had a relatively high MFO induction potential response (9-fold relative to controls). From the results it was concluded that, when using EROD induction as a biomarker, black bream is a suitable fish to use as a bioindicator species for investigations into the health of the Swan-Canning Estuary.

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“…Hence, no unambiguous conclusions could be made from the MFO induction and species feeding habits or contact with sediments. All aforementioned leads to large variations in induction capabilities of MFO enzymes in various fish species using very similar protocols and inducing agents (Kloepper-Sams and Benton, 1994;Focardi et al, 1995;Smith and Gagnon, 1998). A nose carp (Chondrostoma nasus) had the highest level of BaPMO activity in both Sava (36.2 6 4.2 BaPOHmg prot 21 min 21 ) and Mrežnica (5.8 6 0.4 BaPOHmg prot 21 min 21 ) rivers (Fig.…”

Section: Discussionmentioning

confidence: 99%

Detection of mutagens and BaPMO inducers in river water using the Blue Cotton adsorption technique

Britvić

Hackenberger

Jarić

et al. 2012

Environmental Toxicology

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Induction of Mixed Function Oxidase (MFO) activity and bioactivation potential were measured in experimental carp and in native fish species from two rivers with different pollution level (Sava and Mrežnica). The experimental carp were intraperitoneally exposed to various water volume equivalents of Blue Cotton extracts from both rivers. The induction of MFO measured as a Benzo(a)pyrene monooxygenase (BaPMO) activity was increased up to 9.3-fold in experimental carp and up to 11.3-fold in native fish from Sava River, whereas the values from Mrežnica River showed only a slight increase when compared with the control (highest increase of 1.8-fold in nose carp). Accordingly, bioactivation potential using modified Ames test was higher in both experiments with Sava River. Both measured parameters in experimental carp increased in a dose-dependent manner in accordance to river volume equivalents. Different induction potential of native fish species comparable between two rivers confirmed the known possibility of their usage in biomonitoring studies. These results give qualitatively a new support to the idea of using Blue Cotton extraction technique combined with induction of MFO activity and Ames test in fish as a good biomarker for assessing risk of exposure to mutagens/carcinogens such as Polycyclic Aromatic Hydrocarbons (PAHs), especially due to the simplicity and cost-effectiveness of these methods.

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MFO Induction of three Australian fish species

Cited by 10 publications

References 25 publications

Health Status of Sand Flathead (Platycephalus bassensis), Inhabiting an Industrialised and Urbanised Embayment, Port Phillip Bay, Victoria as Measured by Biomarkers of Exposure and Effects

Health Status of Sand Flathead (Platycephalus bassensis), Inhabiting an Industrialised and Urbanised Embayment, Port Phillip Bay, Victoria as Measured by Biomarkers of Exposure and Effects

MFO induction potential of fish species native to the Swan–Canning Estuary, Western Australia

Detection of mutagens and BaPMO inducers in river water using the Blue Cotton adsorption technique

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