Mga2p Is a Putative Sensor for Low Temperature and Oxygen to Induce OLE1 Transcription in Saccharomyces cerevisiae

Nakagawa, Youji; Sakumoto, Naoko; Kaneko, Yoshinobu; Harashima, Satoshi

doi:10.1006/bbrc.2002.6507

Cited by 107 publications

(92 citation statements)

References 46 publications

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“…Unlike in S. cerevisiae, transcription of KlOLE1 (Fig. S5b) was not induced by cobalt chloride or by incubation at low temperature (Nakagawa et al, 2002). (Fig.…”

Section: Expression Of Hypoxic Genes In Gdk/klmga2d Strainmentioning

confidence: 85%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis In the respiratory yeast Kluyveromyces lactis, little is known about the factors regulating the metabolic response to oxygen shortage. After searching for homologues of characterized Saccharomyces cerevisiae regulators of the hypoxic response, we identified a gene that we named KlMGA2, which is homologous to MGA2. The deletion of KlMGA2 strongly reduced both the fermentative and respiratory growth rate and altered fatty acid composition and the unsaturation index of membranes. The reciprocal heterologous expression of MGA2 and KlMGA2 in the corresponding deletion mutant strains suggested that Mga2 and KlMga2 are functional homologues. KlMGA2 transcription was induced by hypoxia and the glucose sensor Rag4 mediated the hypoxic induction of KlMGA2. Transcription of lipid biosynthetic genes KlOLE1, KlERG1, KlFAS1 and KlATF1 was induced by hypoxia and was dependent on KlMga2, except for KlOLE1. Rag4 was required for hypoxic induction of transcription for both KlMga2-dependent (KlERG1) and KlMga2-independent (KlOLE1) structural genes.

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“…Unlike in S. cerevisiae, transcription of KlOLE1 (Fig. S5b) was not induced by cobalt chloride or by incubation at low temperature (Nakagawa et al, 2002). (Fig.…”

Section: Expression Of Hypoxic Genes In Gdk/klmga2d Strainmentioning

confidence: 85%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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“…Suutari et al (1990), however, showed that the UFA/SFA ratio of Saccharomyces is unaffected by growth temperature. On the other hand, the strong induction of OLE1 expression upon shifting yeast from 30°to 10° (Nakagawa et al 2002) strongly implicates the UFA/SFA ratio in yeast membrane fluidity control. Although yeast are poikilotherms, as are all microbes, an understanding of the contributions of C16/C18 and UFA/SFA ratios to yeast membrane fluidity is likely to be relevant to homeotherms as well.…”

Section: Discussionmentioning

confidence: 99%

The Sensitivity of Yeast Mutants to Oleic Acid Implicates the Peroxisome and Other Processes in Membrane Function

et al. 2007

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The peroxisome, sole site of b-oxidation in Saccharomyces cerevisiae, is known to be required for optimal growth in the presence of fatty acid. Screening of the haploid yeast deletion collection identified 130 genes, 23 encoding peroxisomal proteins, necessary for normal growth on oleic acid. Oleate slightly enhances growth of wild-type yeast and inhibits growth of all strains identified by the screen. Nonperoxisomal processes, among them chromatin modification by H2AZ, Pol II mediator function, and cell-wall-associated activities, also prevent oleate toxicity. The most oleate-inhibited strains lack Sap190, a putative adaptor for the PP2A-type protein phosphatase Sit4 (which is also required for normal growth on oleate) and Ilm1, a protein of unknown function. Palmitoleate, the other main unsaturated fatty acid of Saccharomyces, fails to inhibit growth of the sap190D, sit4D, and ilm1D strains. Data that suggest that oleate inhibition of the growth of a peroxisomal mutant is due to an increase in plasma membrane porosity are presented. We propose that yeast deficient in peroxisomal and other functions are sensitive to oleate perhaps because of an inability to effectively control the fatty acid composition of membrane phospholipids.

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“…Transcription of the Sk-FAD2 gene in S. kluyveri Because transcriptional expression of fatty acid desaturase genes of several fungi are known to be induced by low-temperature stress, 12,[33][34][35] we investigated whether transcription of Sk-FAD2 is regulated by low temperatures. After culture at 30 C, S. kluyveri IFO1893 was transferred to 10 C and then total RNA was isolated from these cells cultured at 10 C for 0, 1, 2, 4, 6, or 8 h. Northern blot analysis was done using the Sk-FAD2 gene as a probe.…”

Section: )mentioning

confidence: 99%

“…5,11) Furthermore, in S. cerevisiae, low-temperature stress was found to affect expression of OLE1 gene. 12) On the other hand, the Á6, Á12, and Á15 fatty acid desaturase genes of fungi have not been as extensively studied as the OLE1 genes. Recently, from three filamentous fungi, Aspergillus, Mortierella, and Mucor species, Á12 desaturase genes were isolated and their functions analyzed.…”

mentioning

confidence: 99%

Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function

Watanabe

Oura

Sakai

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Mga2p Is a Putative Sensor for Low Temperature and Oxygen to Induce OLE1 Transcription in Saccharomyces cerevisiae

Cited by 107 publications

References 46 publications

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

The Sensitivity of Yeast Mutants to Oleic Acid Implicates the Peroxisome and Other Processes in Membrane Function

Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function

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