MgtE is a dual-function protein in Pseudomonas aeruginosa

Coffey, Barbara M.; Akhand, Saeed Salehin; Anderson, Gregory G.

doi:10.1099/mic.0.075275-0

Cited by 14 publications

(14 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S1A in the supplemental material). This increase is concordant with transcript levels seen in physiologically relevant concentrations of antibiotics and magnesium (29,30). Similar results were obtained with P. aeruginosa strain PA14 (Fig.…”

Section: Resultssupporting

confidence: 89%

“…We also demonstrate that mgtE acts exclusively through the RsmA/RsmY/RsmZ signaling pathway to inhibit ExsAmediated T3SS gene transcription. Because mgtE transcription is significantly upregulated by growth under low-Mg 2ϩ conditions and in the presence of some antibiotics (29,30), this pathway may provide a mechanism for P. aeruginosa to modulate T3SS gene expression in response to signals encountered during infections.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Type III Secretion System Gene Expression by Stimulating rsmYZ Transcription

et al. 2017

Self Cite

View full text Add to dashboard Cite

causes numerous acute and chronic opportunistic infections in humans. One of its most formidable weapons is a type III secretion system (T3SS), which injects powerful toxins directly into host cells. The toxins lead to cell dysfunction and, ultimately, cell death. Identification of regulatory pathways that control T3SS gene expression may lead to the discovery of novel therapeutics to treat infections. In a previous study, we found that expression of the magnesium transporter gene inhibits T3SS gene transcription. MgtE-dependent inhibition appeared to interfere with the synthesis or function of the master T3SS transcriptional activator ExsA, although the exact mechanism was unclear. We now demonstrate that expression acts through the GacAS two-component system to activate and transcription. This event ultimately leads to inhibition of translation. This inhibitory effect is specific to as translation of other genes in the operon is not inhibited by Moreover, our data reveal that MgtE acts solely through this pathway to regulate T3SS gene transcription. Our study reveals an important mechanism that may allow to fine-tune T3SS activity in response to certain environmental stimuli. The type III secretion system (T3SS) is a critical virulence factor utilized by numerous Gram-negative bacteria, including , to intoxicate and kill host cells. Elucidating T3SS regulatory mechanisms may uncover targets for novel anti- therapeutics and provide deeper understanding of bacterial pathogenesis. We previously found that the magnesium transporter MgtE inhibits T3SS gene transcription in In this study, we describe the mechanism of MgtE-dependent inhibition of the T3SS. Our report also illustrates how MgtE might respond to environmental cues, such as magnesium levels, to fine-tune T3SS gene expression.

show abstract

Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Type III Secretion System Gene Expression by Stimulating rsmYZ Transcription

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The structure of MgtE from Thermus thermophilus has been determined in the presence of Mg 21 (Hattori et al, 2007;2009): it is a homodimer containing a C-terminal ion pore embedded in the cytoplasmic membrane linked to a large intracellular N-terminal regulatory region. MgtE is not essential for P. aeruginosa growth even under low magnesium conditions (Coffey et al, 2014), suggesting that CorA or MgtA/MgtB are more important for Mg 21 import. According to in silico analysis, MgtE of P. aeruginosa has structural similarities with MgtE of Thermus thermophilus (Coffey et al, 2014).…”

Section: Magnesium (Mg 21 )mentioning

confidence: 94%

“…It is not as widespread among bacteria as the ubiquitous CorA (Smith and Maguire, 1995). According to in silico analysis, MgtE of P. aeruginosa has structural similarities with MgtE of Thermus thermophilus (Coffey et al, 2014). The pore is formed by four membrane-spanning a-helices per subunit (a total of 10 transmembrane helices in the dimeric organization).…”

Section: Magnesium (Mg 21 )mentioning

confidence: 99%

“…The pore is formed by four membrane-spanning a-helices per subunit (a total of 10 transmembrane helices in the dimeric organization). According to in silico analysis, MgtE of P. aeruginosa has structural similarities with MgtE of Thermus thermophilus (Coffey et al, 2014). MgtE is not essential for P. aeruginosa growth even under low magnesium conditions (Coffey et al, 2014), suggesting that CorA or MgtA/MgtB are more important for Mg 21 import.…”

Section: Magnesium (Mg 21 )mentioning

confidence: 99%

See 1 more Smart Citation

An overview of the biological metal uptake pathways in Pseudomonas aeruginosa

Schalk

Cunrath

2016

Environmental Microbiology

View full text Add to dashboard Cite

Biological metal ions, including Co, Cu, Fe, Mg, Mn, Mo, Ni and Zn ions, are necessary for the survival and the growth of all microorganisms. Their biological functions are linked to their particular chemical properties: they play a role in structuring macromolecules and/or act as co-factors catalyzing diverse biochemical reactions. These metal ions are also essential for microbial pathogens during infection: they are involved in bacterial metabolism and various virulence factor functions. Therefore, during infection, bacteria need to acquire biological metal ions from the host such that there is competition for these ions between the bacterium and the host. Evidence is increasingly emerging of "nutritional immunity" against pathogens in the hosts; this includes strategies making access to metals difficult for infecting bacteria. It is clear that biological metals play key roles during infection and in the battle between the pathogens and the host. Here, we summarize current knowledge about the strategies used by Pseudomonas aeruginosa to access the various biological metals it requires. P. aeruginosa is a medically significant Gram-negative bacterial opportunistic pathogen that can cause severe chronic lung infections in cystic fibrosis patients and that is responsible for nosocomial infections worldwide.

show abstract