Aim
The aims of this study were to estimate human leukocyte antigen (HLA)‐B allele frequency, to identify alleles associated with ankylosing spondylitis (AS), and to explore manifestations in various HLA‐B*27 in Thai AS patients.
Methods
This was a cross‐sectional study. Thai patients older than 18 years with diagnosed AS according to modified New York criteria who visited Siriraj Hospital (Bangkok, Thailand) were consecutively enrolled. HLA‐B alleles were determined by reverse sequence‐specific oligonucleotide assays, and were assigned at a 4‐digit resolution. HLA‐B alleles of 334 unrelated healthy Thai donors who participated in a previous phase 2b dengue vaccine clinical trial were included as controls. Odds ratio (OR) and Fisher's exact test were used to estimate association between allele and AS. The P value significance threshold was calculated according to Bonferroni.
Results
Among the 88 patients who were recruited, 34 HLA‐B alleles were identified, and all patients were heterozygous. The prevalence of HLA‐B*27 was 89.8%, and 4 alleles of HLA‐B*27 were identified. HLA‐B*27:04 (OR: 39.4, P < .0001) and HLA‐B*27:05 (OR: 13.8, P = .0011) were associated with AS. In contrast, HLA‐B*27:06 was not found to be associated with AS (OR: 0.4, P = .241). AS patients carrying HLA‐B*27:04 were more likely to have enthesitis and younger age at onset than those carrying HLA‐B*27:05.
Conclusions
HLA‐B*27:04 and HLA‐B*27:05 were both found to be strongly associated with Thai AS. HLA‐B*27:06 showed a neutral allele for Thai AS. AS patients with HLA‐B*27:04 had more enthesitis and younger age at onset than those with HLA‐B*27:05.