MHC class I A region diversity and polymorphism in macaque species

Otting, Nel; Vos-Rouweler, Annemiek J. M. de; Heijmans, Corrine M. C.; Groot, Natasja G. de; Doxiadis, Gaby G. M.; Bontrop, Ronald E.

doi:10.1007/s00251-007-0201-2

Cited by 103 publications

(161 citation statements)

References 51 publications

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“…One of these alleles, Mamu-A1*2601, was also recently detected in Chinese rhesus macaques by Otting et al (2007). The Mamu-A4*1403 allele reported here is consistent with being an Indian origin macaque allele, lacking the stop codon in exon 5 that was present in the only previously reported Chinese Mamu-A4 allele (Otting et al 2007). The degree of Mamu-B cDNA sharing between rhesus macaques of different geographic origin is not currently known, and there is only limited information on Mamu-A allele sharing.…”

Section: Potential Admixture Of Chinese-and Indian-origin Rhesus Macasupporting

confidence: 87%

“…Of these 41 sequences, 8 had been previously reported in rhesus macaques of Indian origin with at least partial cDNA sequences already available in GenBank. In addition, six were identical to alleles recently reported in Chinese rhesus macaques (Otting et al 2007). The remaining 27 cDNAs described in this study have not been reported previously.…”

Section: Summary Of Mhc Class I Cdnas Identifiedsupporting

confidence: 77%

“…Prior studies have reported exon 2 sequences and frequencies for class II alleles at the DR, DQ, and DP loci and examined Chinese rhesus macaques for the frequency of Mamu-A*01 (Doxiadis et al 2003, Viray et al 2001, Vogel et al 1995. Recently, 59 Mamu-A partial alleles (lacking approximately 30 bases from the 5′ end of the coding region) were reported for Chinese rhesus macaques (Otting et al 2007). It is not clear whether these 59 alleles represent a small or large percentage of MHC allelic diversity in this population.…”

Section: Introductionmentioning

confidence: 99%

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Identification of MHC class I sequences in Chinese-origin rhesus macaques

et al. 2007

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The rhesus macaque (Macaca mulatta) is an excellent model for human disease and vaccine research. Two populations exhibiting distinctive morphological and physiological characteristics, Indian-and Chinese-origin rhesus macaques, are commonly used in research. Genetic analysis has focused on the Indian macaque population, but the accessibility of these animals for research is limited. Due to their greater availability, Chinese rhesus macaques are now being used more frequently, particularly in vaccine and biodefense studies, although relatively little is known about their immunogenetics. In this study, we discovered major histocompatibility complex (MHC) class I cDNAs in 12 Chinese rhesus macaques and detected 41 distinct Mamu-A and Mamu-B sequences. Twenty-seven of these class I cDNAs were novel, while six and eight of these sequences were previously reported in Chinese and Indian rhesus macaques, respectively. We then performed microsatellite analysis on DNA from these 12 animals, as well as an additional 18 animals, and developed sequence specific primer PCR (PCR-SSP) assays for eight cDNAs found in multiple animals. We also examined our cohort for potential admixture of Chinese and Indian origin animals using a recently developed panel of single nucleotide polymorphisms (SNPs). The discovery of 27 novel MHC class I sequences in this analysis underscores the genetic diversity of Chinese rhesus macaques and contributes reagents that will be valuable for studying cellular immunology in this population.

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Section: Potential Admixture Of Chinese-and Indian-origin Rhesus Macasupporting

confidence: 87%

Section: Summary Of Mhc Class I Cdnas Identifiedsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

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Identification of MHC class I sequences in Chinese-origin rhesus macaques

et al. 2007

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“…But the critical reagents for vaccine research, such as MHC class I tetramers, are currently only designed on the immunogenetic background of Indian rhesus macaques, while the two geographic macaque populations share few common MHC class I alleles (2,10,(17)(18)(19). This study was intended to set up a procedure for constructing new tetramers on the immunogenetic background of Chinese macaques.…”

Section: Discussionmentioning

confidence: 99%

Construction of Soluble Mamu-B*1703, a Class I Major Histocompatibility Complex of Chinese Rhesus Macaques, Monomer and Tetramer Loaded with a Simian Immunodeficiency Virus Peptide

Ouyang

Wang

et al. 2009

Cell Mol Immunol

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Chinese-descent rhesus macaques have become more prevalent for HIV infection and vaccine investigation than Indian-origin macaques. Most of the currently available data and reagents such as major histocompatibility complex (MHC) class I tetramers, however, were derived from Indian-origin macaques due to the dominant use of these animals in history. Although there are significant differences in the immunogenetic background between the two macaque populations, they share a few of common MHC class I alleles. We reported in this study the procedure for preparation of a soluble Mamu-B*1703 (a MHC class I molecule of Chinese macaques) monomer and tetramer loaded with a dominant simian immunodeficiency virus (SIV) epitope IW9 (IRYPKTFGW) that was identified to be Mamu-B*1701-restricted in Indian macaques. The DNA fragment encoding the Mamu-B*1703 extracellular domain fused with a BirA substrate peptide (BSP) was amplified from a previously cloned cDNA and inserted into a prokaratic expression vector. In the presence of the antigenic peptide IW9 and light chain  2 -microglobulin, the expressed heavy chain was refolded into a soluble monomer. After biotinylation, four monomers were polymerized as a tetramer by phycoerythrin-conjugated streptavidin. The tetramer, having been confirmed to have the right conformation, was a potential tool for investigation of antigen-specific CD8 + T-lymphocytes in SIV vaccine models of Chinese macaques. And our results also suggested that some antigenic peptides reported in Indian-origin macaques could be directly recruited as ligands for construction of Chinese macaque MHC tetramers. Cellular & Molecular Immunology. 2009;6(2):117-122.

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“…Before entering the study, all animals were confirmed to be negative for simian immunodeficiency virus (SIV), simian T-cell lymphotropic virus, and simian retrovirus type D. The MHC class I alleles were determined for all animals. RNA isolation, reverse transcription-PCR (RT-PCR), and cloning and sequencing of macaque class I A and B alleles were described elsewhere (26,27). Unreported alleles, of which at least three full-length clones were present, were submitted to the EMBL-EBI database and also to the nonhuman primate MHC section of the Immuno Polymorphism Database (for official designations, see reference 28).…”

Section: Methodsmentioning

confidence: 99%

Immunization with Recombinant HLA Classes I and II, HIV-1 gp140, and SIV p27 Elicits Protection against Heterologous SHIV Infection in Rhesus Macaques

Mörner

Jansson

Bunnik

et al. 2011

J Virol

Self Cite

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Major histocompatibility complex (MHC) molecules expressed on the surface of human immunodeficiency virus (HIV) are potential targets for neutralizing antibodies. Since MHC molecules are polymorphic, nonself MHC can also be immunogenic. We have used combinations of novel recombinant HLA class I and II and HIV/simian immunodeficiency virus (SIV) antigens, all linked to dextran, to investigate whether they can elicit protective immunity against heterologous simian/human immunodeficiency virus (SHIV) challenge in rhesus macaques. Three groups of animals were immunized with HLA (group 1, n ‫؍‬ 8), trimeric YU2 HIV type 1 (HIV-1) gp140 and SIV p27 (HIV/SIV antigens; group 2, n ‫؍‬ 8), or HLA plus HIV/SIV antigens (group 3, n ‫؍‬ 8), all with Hsp70 and TiterMax Gold adjuvant. Another group (group 4, n ‫؍‬ 6) received the same vaccine as group 3 without TiterMax Gold. Two of eight macaques in group 3 were completely protected against intravenous challenge with 18 50% animal infective doses (AID 50 ) of SHIV-SF162P4/C grown in human cells expressing HLA class I and II lineages represented in the vaccine, while the remaining six macaques showed decreased viral loads compared to those in unimmunized animals. Complement-dependent neutralizing activity in serum and high levels of anti-HLA antibodies were elicited in groups 1 and 3, and both were inversely correlated with the plasma viral load at 2 weeks postchallenge. Antibody-mediated protection was strongly supported by the fact that transfer of pooled serum from the two challenged but uninfected animals protected two naïve animals against repeated low-dose challenge with the same SHIV stock. This study demonstrates that immunization with recombinant HLA in combination with HIV-1 antigens might be developed into an alternative strategy for a future AIDS vaccine.

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MHC class I A region diversity and polymorphism in macaque species

Cited by 103 publications

References 51 publications

Identification of MHC class I sequences in Chinese-origin rhesus macaques

Identification of MHC class I sequences in Chinese-origin rhesus macaques

Construction of Soluble Mamu-B*1703, a Class I Major Histocompatibility Complex of Chinese Rhesus Macaques, Monomer and Tetramer Loaded with a Simian Immunodeficiency Virus Peptide

Immunization with Recombinant HLA Classes I and II, HIV-1 gp140, and SIV p27 Elicits Protection against Heterologous SHIV Infection in Rhesus Macaques

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