MHC-DRB1/DQB1 Gen Polimorfizmi ve Çin Merinos Koyununda Kistik Echinococcosise Dirençle İlişkisi

Abstract

In this study, we used the single strand conformation polymorphism (SSCP) method to analyze the associations between polymorphisms of exon 2 of the DRB1 and DQB1 genes and Cystic Echinococcosis (CE) in Chinese Merino sheep. We examined 96 CE positive sheep and 115 negative sheep in this study. The results showed thatthere were 53 genotypes controlled by 22 alleles in the DRB1 gene and 47 genotypes controlled by 17 alleles in the DQB1 gene. The comparison of allele frequencies in the CE positive and negative animals revealed that DRB1 alleles k (P<0.05), q , t (P<0.01) and alleles a , l (P<0.01). These results indicate a strong association between these alleles k,q,t and CE resistance, the alleles a,l are associated with CE susceptibility. We analyzed the DRB1 genotype frequencies and found that the genotypes KK and TT (P<0.05) are associated with CE resistance, while AA, LL (P<0.01), and AL (P<0.05) were related to CE susceptibility. In DQB1 exon 2 the d and e alleles (P<0.01) were related to CE resistance, while c and k (P<0.01) were significantly related to CE susceptibility. We analyzed the DQB1 genotype frequencies and found that DD and EG (P<0.01) were associated with CE resistance, while genotypes CC, KK, and CK (P<0.01) were associated with CE susceptibility. Using haplotype analysis and artificially infection tests with Echinococcus granulosus (EG), we found that the DRB1-TT/DQB1-EE was a CE resistant haplotype in Chinese Merino sheep. Keywords: DRB1, DQB1Echinococcosis Resistance, Susceptibility MHC-DRB1/DQB1 Gen Polimorfizmi ve Çin Merinos Koyununda Kistik Echinococcosise Dirençle İlişkisi