This research contributes to the comprehension of nanofluid behaviour through a wavy channel, emphasizing the significance of considering diverse influences in the modelling process. The study explores the collective influence of pressure gradient variation, magnetic field, porosity, channel waviness, nanoparticle concentration, and heat transfer on nano-blood flow in a two-dimensional wavy channel. In contrast to prior research assuming a constant pulsatile pressure gradient during channel waviness, this innovative study introduces a variable pressure gradient, significantly influencing several associated parameters. The mathematical model characterizing nano-blood flow in a horizontally wavy channel is solved using the perturbation technique. Analytical solutions for fundamental variables such as stream function, velocity, wall shear stress, pressure gradient, and temperature are visually depicted across different physical parameters values. The findings obtained for differing parameter values in the given problem demonstrate a significant influence of the amplitude ratio parameter of channel waviness, Hartmann number of the magnetic field, permeability parameter of the porous medium, volume fraction of nanoparticles, radiation parameter, Prandtl number, and the suction/injection parameter on the flow dynamics. The simulations provide valuable insights into the decrease in velocity with increasing magnetic field and its increase with higher permeability. Additionally, the temperature is observed to escalate with a rising nanoparticle volume fraction and radiation parameter, while it declines with increasing Prandtl number.