MhYTP1 and MhYTP2 from Apple Confer Tolerance to Multiple Abiotic Stresses in Arabidopsis thaliana

Wang, Na; Guo, Tianli; Wang, Ping; Sun, Xun; Shao, Yun; Jia, Xin; Liang, Bowen; Gong, Xiaoqing; Ma, Fengwang

doi:10.3389/fpls.2017.01367

Cited by 20 publications

(17 citation statements)

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“…The selection and creation of wheat germplasm with tolerance to multiple abiotic and biotic stresses is a key challenge for wheat breeding [ 42 – 56 ]. Our results and those of previous studies reveal that the expression of YTH gene is involved in the responses to various stresses and in plant development [ 10 , 13 , 15 , 17 , 21 ]. Therefore, the function of wheat YTH genes in the response to abiotic stresses merits further study, and these YTH genes might be valuable for wheat genetic improvement.…”

Section: Discussionsupporting

confidence: 84%

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

et al. 2020

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Background: N6-Methyladenosine (m6A) is the most widespread RNA modification that plays roles in the regulation of genes and genome stability. YT521-B homology (YTH) domain-containing RNA-binding proteins are important RNA binding proteins that affect the fate of m6A-containing RNA by binding m6A. Little is known about the YTH genes in common wheat (Triticum aestivum L.), one of the most important crops for humans. Results: A total of 39 TaYTH genes were identified in common wheat, which are comprised of 13 homologous triads, and could be mapped in 18 out of the 21 chromosomes. A phylogenetic analysis revealed that the TaYTHs could be divided into two groups: YTHDF (TaDF) and YTHDC (TaDC). The TaYTHs in the same group share similar motif distributions and domain organizations, which indicates functional similarity between the closely related TaYTHs. The TaDF proteins share only one domain, which is the YTH domain. In contrast, the TaDCs possess three C3H1-type zinc finger repeats at their N-termini in addition to their central YTH domain. In TaDFs, the predicated aromatic cage pocket that binds the methylysine residue of m6A is composed of tryptophan, tryptophan, and tryptophan (WWW). In contrast, the aromatic cage pocket in the TaDCs is composed of tryptophan, tryptophan, and tyrosine (WWY). In addition to the general aspartic acid or asparagine residue used to form a hydrogen bond with N 1 of m6A, histidine might be utilized in some TaDFb proteins. An analysis of the expression using both online RNA-Seq data and quantitative real-time PCR verification revealed that the TaDFa and TaDFb genes are highly expressed in various tissues/organs compared with that of TaDFcs and TaDCs. In addition, the expression of the TaYTH genes is changed in response to various abiotic stresses. Conclusions: In this study, we identified 39 TaYTH genes from common wheat. The phylogenetic structure, chromosome distribution, and patterns of expression of these genes and their protein structures were analyzed. Our results provide a foundation for the functional analysis of TaYTHs in the future.

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Section: Discussionsupporting

confidence: 84%

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

et al. 2020

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“…However, there is limited direct evidence showing that YTH genes play a role in different stress responses in plants. Overexpression of MhYTP1 or MhYTP2 makes plants more sensitive to NaCl, but more resistant to nutrient deficits (Wang et al, 2017b). In addition, Wang et al (2017c) reported that, compared with wild-type plants, plants overexpressing MhYTP1 or MhYTP2 were more sensitive to heat stress and high salinity but were more resistant to waterlogging, chilling, drought, and nutrient deficiency conditions.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Identification and Expression Analysis of the YTH Domain-containing RNA-binding Protein Family in Citrus Sinensis

Ouyang¹,

Duan²,

Mi³

et al. 2019

J. Amer. Soc. Hort. Sci.

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In eukaryotic systems, messenger RNA regulations, including splicing, 3′-end formation, editing, localization, and translation, are achieved by different RNA-binding proteins and noncoding RNAs. The YTH domain is a newly identified RNA-binding domain that was identified by comparing its sequence with that of splicing factor YT521-B. Previous study showed that the YTH gene plays an important role in plant resistance to abiotic and biotic stress. In this study, 211 YTH genes were identified in 26 species that represent four major plant lineages. Phylogenetic analysis revealed that these genes could be divided into eight subgroups. All of the YTH genes contain a YT521 domain and have different structures. Ten YTH genes were identified in navel orange (Citrus sinensis). The expression profiles of these CitYTH genes were analyzed in different tissues and at different fruit developmental stages, and CitYTH genes displayed distinct expression patterns under heat, cold, salt, and drought stress. Furthermore, expression of the CitYTH genes in response to exogenous hormones was measured. Nuclear localization was also confirmed for five of the proteins encoded by these genes after transient expression in Nicotiana benthamiana cells. This study provides valuable information on the role of CitYTHs in the signaling pathways involved in environmental stress responses in Citrus.

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“…Seed ethylene priming allowed the recall of long-lasting stress defensive memory, which decreased the Ethylene perception signal's gene expression. The Ethylene perception signal and root to shoot ABA secondary signal involve a dialogue between ABA, IAA, and SA, and the ABA is the dominant hormone regarding its role in plant drought tolerance (Wang et al 2017b;Zhu et al 2019;Harb et al 2020). The production of ABA in the root hair and then mobilized to the leaves will maintain plant water status through stromal regulation (Zhang et al 2018), and then postpone the development of water de cit in the aboveground (Liu et al 2005).…”

Section: Seed Ethylene Priming Recalling Long-lasting Stress Memory Tmentioning

confidence: 99%

Thirty years of genetic progress combined with seed ethylene priming improved drought resistance of wheat through long-term drought stress memory

Yang

Zhao

et al. 2021

Preprint

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Knowledge of short-term physiological adaption of wheat to soil water deficit is well understood, but little is known about seed ethylene priming effect on long-term drought stress memory of dryland winter wheat. The 42 leading and new cultivars released between 1992 to 2017 were subjected to drought (45% Field water-holding capacity), and well-watered (75% Field water-holding capacity) conditions aim to screen cultivars with contrasting drought resistance and grain yield. Seeds primed with ethylene were subjected to both PEG-8000 and pure water to uncover ethylene-induced stress memory at both physiological and organ levels. Results showed that the soil water deficit (45%FC) that occurred at the tillering stage resulted in 3.2% to 67.4% yield loss for 42 cultivars, which was determined mainly by the decrease in the fertile spike. Seed ethylene priming maintained leaf water by reducing root volume and dry weight, which played a crucial role in drought avoidance. Seed ethylene priming decreased malondialdehyde content by regulating auxin and abscisic acid signaling, reactive oxygen species scavenging capability, and osmotic regulation, which plays a crucial role in drought tolerance. Seed ethylene priming improved drought tolerance of the wheat through metabolic modification of carbon metabolism, glutathione metabolism, and phenylpropanoid biosynthesis . The seed ethylene priming induced long-term stress memory that improved tillering capacity and reduced wheat spikelets abortion, which provided extra 0.3 t ha -1 of grain yield. These results suggested that seed ethylene priming allowed the recall of long-lasting stress defensive memory, increasing grain yield by both drought avoidance and drought tolerance.

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MhYTP1 and MhYTP2 from Apple Confer Tolerance to Multiple Abiotic Stresses in Arabidopsis thaliana

Cited by 20 publications

References 39 publications

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

Genome-wide Identification and Expression Analysis of the YTH Domain-containing RNA-binding Protein Family in Citrus Sinensis

Thirty years of genetic progress combined with seed ethylene priming improved drought resistance of wheat through long-term drought stress memory

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