mi‑R4435‑2HG promotes proliferation and inhibits apoptosis of cancer cells in ovarian carcinoma by upregulating ROCK2

Hu, Jinghui; Wang, Li; Zhao, Wei; Huang, Yujie; Wang, Zhe; Shen, Heping

doi:10.3892/ol.2019.11204

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…The present study identified ROCK2 as a novel target gene of miR-181a-5p in CRC cells. ROCK2 has been reported to play a vital role in multiple cellular processes, such as proliferation ( 37 ), apoptosis ( 38 ), DNA repair ( 39 ) and invasion ( 40 ). A study indicated that ROCK2 contributed to the invasion and metastasis of CRC ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR‑181a‑5p/ROCK2 axis

Chi

Liu

et al. 2021

Oncol Rep

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Aberrant expression of circular RNAs (circRNAs) has been demonstrated to be related to the development of colorectal cancer (CRC), the third most common cancer worldwide. However, the mechanism of the effect of circRNA NOP2/Sun domain family, member 2 (circNSUN2) on the malignant biological behavior of CRC remains unclear. In the present study, the expression of circNSUN2 and microRNA (miR)-181a-5p was detected by RT-qPCR. The expression of Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) was measured by western blotting. Cell proliferation was detected by CCK-8 assay. The cell apoptosis rate was measured by flow cytometry. Cell migration ability was evaluated by Transwell assay. The interactions between circNSUN2, miR-181a-5p and ROCK2 were verified by dual-luciferase reporter assay. The results revealed that circNSUN2 was highly expressed in CRC tissues and cell lines. Knockdown of circNSUN2 inhibited the malignant biological behavior of CRC in vivo and in vitro . Moreover, miR-181a-5p was revealed to be a target gene of circNSUN2, and the expression of ROCK2 was negatively regulated by miR-181a-5p. Knockdown of circNSUN2 inhibited proliferation and migration, and induced apoptosis of CRC cells and suppressed tumor growth by targeting miR-181a-5p to decrease ROCK2 expression. In conclusion, circNSUN2 promoted the progression of CRC by sponging miR-181a-5p to increase the expression of ROCK2.

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Section: Discussionmentioning

confidence: 99%

circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR‑181a‑5p/ROCK2 axis

Chi

Liu

et al. 2021

Oncol Rep

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“…ROCK2 and CTSB gains or amplifications were shared among six samples (both STUMPs and LMSs). ROCK2 (Rho associated coiled-coil containing protein kinase 2) upregulation has been described in human glioblastoma CSCs [13], in gastric cancer [14], as well as in ovarian cancer samples [15]. Moreover, ROCK2 expression level has been associated with worse prognosis in osteosarcoma tissues [16].…”

Section: Discussionmentioning

confidence: 99%

Genomic and Epigenomic Profile of Uterine Smooth Muscle Tumors of Uncertain Malignant Potential (STUMPs) Revealed Similarities and Differences with Leiomyomas and Leiomyosarcomas

Conconi

Redaelli

Lissoni

et al. 2021

IJMS

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Uterine smooth muscle tumors of uncertain malignant potential (STUMPs) represent a heterogeneous group of tumors that cannot be histologically diagnosed as unequivocally benign or malignant. For this reason, many authors are working to obtain a better definition of diagnostic and prognostic criteria. In this work, we analyzed the genomic and epigenomic profile of uterine smooth muscle tumors (USMTs) in order to find similarities and differences between STUMPs, leiomyosarcomas (LMSs) and leiomyomas (LMs), and possibly identify prognostic factors in this group of tumors. Array-CGH data on 23 USMTs demonstrated the presence of a more similar genomic profile between STUMPs and LMSs. Some genes, such as PRKDC and PUM2, with a potential prognostic value, were never previously associated with STUMP. The methylation data appears to be very promising, especially with regards to the divergent profile found in the sample that relapsed, characterized by an overall CGI hypomethylation. Finally, the Gene Ontology analysis highlighted some cancer genes that could play a pivotal role in the unexpected aggressive behavior that can be found in some of these tumors. These genes could prove to be prognostic markers in the future.

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“…miR-4435-2HG, an oncogenic lncRNA, was shown to promote the expression of ROCK2, and inhibited the apoptotic death of ovarian cancer cells (57). miR4435-2HG overexpression induced the upregulation of ROCK2 in ovarian cancer UWB1.289 cells (57). Similarly, other lncRNAs that promote carcinogenesis have also been identified in ovarian cancer.…”

Section: Regulation Of Rock2 By Lncrnas: Oncogenic Rolementioning

confidence: 98%

Regulation of ROCK1/2 by long non‑coding RNAs and circular RNAs in different cancer types (Review)

et al. 2022

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Recent breakthroughs in high-throughput technologies have enabled the development of a better understanding of the functionalities of rho-associated protein kinases (ROCKs) under various physiological and pathological conditions. Since their discovery in the late 1990s, ROCKs have attracted the attention of interdisciplinary researchers due to their ability to pleiotropically modulate a myriad of cellular mechanisms. A rapidly growing number of published studies have started to shed light on the mechanisms underlying the regulation of ROCK1 and ROCK2 via long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) in different types of cancer. Detailed analyses have suggested that lncRNAs may be characteristically divided into oncogenic and tumor suppressor lncRNAs. Several exciting recent discoveries have also indicated how different lncRNAs and circRNAs modulate ROCK1/2 and mediate multistep cancer onset and progression. The present review chronicles the major advances that have been made in our understanding of the regulatory role of ROCK1/2 in different types of cancer, and how wide-ranging lncRNAs and circRNAs potentiate ROCK-driven signaling by blocking the targeting activities of tumor suppressor microRNAs.

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mi‑R4435‑2HG promotes proliferation and inhibits apoptosis of cancer cells in ovarian carcinoma by upregulating ROCK2

Cited by 5 publications

References 17 publications

circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR‑181a‑5p/ROCK2 axis

circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR‑181a‑5p/ROCK2 axis

Genomic and Epigenomic Profile of Uterine Smooth Muscle Tumors of Uncertain Malignant Potential (STUMPs) Revealed Similarities and Differences with Leiomyomas and Leiomyosarcomas

Regulation of ROCK1/2 by long non‑coding RNAs and circular RNAs in different cancer types (Review)

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