2004
DOI: 10.1038/sj.gene.6364103
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MICA intron 1 sequences of conserved extended HLA haplotypes: implications for sequencing-based typing

Abstract: The human major histocompatibility complex (MHC) class I chain-related gene A (MICA) has a high degree of genetic diversity. Several methods have been used in MICA typing. Recent studies reported different results for the same reference cell lines typed by different methods. By searching the GenBank, we found an indel polymorphism in MICA intron 1 corresponding to the area where one of the sequencing-based typing primers used by others is located. We investigated this polymorphism in 43 reference samples by pr… Show more

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Cited by 8 publications
(9 citation statements)
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“…In all instances in which intervening DNA on independent examples of CEHs has been studied, it has been identical or has manifested highly restricted variation [10, 37-39, 46 -52], including SNPs [53,54]. Wherever analyzed, each CEH carries a specific allele (or, at most, a few closely related specific alleles) determined from DNA [30].…”
Section: Resultsmentioning
confidence: 99%
“…In all instances in which intervening DNA on independent examples of CEHs has been studied, it has been identical or has manifested highly restricted variation [10, 37-39, 46 -52], including SNPs [53,54]. Wherever analyzed, each CEH carries a specific allele (or, at most, a few closely related specific alleles) determined from DNA [30].…”
Section: Resultsmentioning
confidence: 99%
“…These primers were designed in accordance with the sequences of each intron that flank the exons of the MICA gene (GenBank Database). We have avoided the polymorphic locations in the different introns that could induce skew in the results [25, 26]. Amplification was conducted with AmpliTaqGold (Applied Biosystems, Foster City, CA, USA) and PCR thermocycling conditions were 95 °C for 7 min.…”
Section: Methodsmentioning
confidence: 99%
“…We modified the original primers slightly to increase their specificity for MICA to avoid any amplification of MICB. 127 The modified generic primers were MICF 5 0 -TCC TCG TTC TTG TCC CTT TGC CCG and MICR . This area of intron 1 has an indel polymorphism and causes preferential amplification (and subsequent allelic dropout) in samples heterozygote for certain MICA alleles.…”
Section: Lta Snpmentioning
confidence: 99%