Mice do not accumulate muscle lipid in response to dietary conjugated linoleic acid1

Kanosky, Kayla M.; Ippagunta, Siri Manasa; Barnes, Kimberly M

doi:10.2527/jas.2013-6407

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…Therefore we measured serum ketone bodies as an indication of fatty acid oxidation, but did not find any differences between dietary groups. We previously have showed that CPT activity was decreased by CLA in the CO-fed mice [25]. Hence it appears that lipid oxidation is not being enhanced in the CO ?…”

Section: Discussionmentioning

confidence: 90%

Dietary CLA‐induced Lipolysis Is Delayed in Soy Oil‐Fed Mice Compared to Coconut Oil‐Fed Mice

Ippagunta¹,

Angius²,

Sanda³

et al. 2013

Lipids

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Conjugated linoleic acid (CLA) has been shown to cause a reduction in obesity in several species. CLA-induced body fat loss is enhanced when mice are fed coconut oil (CO) and involves increased lipolysis. The objective of this paper was to determine if the CLA-induced lipolysis in mice fed with different oil sources was time-dependent. Mice were fed 7 % soybean oil (SO) or CO diets for 6 week and then supplemented with 0 or 0.5 % CLA for 3, 7, 10 or 14 days. Body fat and ex-vivo lipolysis was determined. Body fat was reduced by CO on day 7 (P < 0.01) and in both CO and SO-fed mice (P < 0.05) in response to CLA on d14. Lipolysis was increased by CLA in CO-fed mice (P < 0.01) but not in SO-fed mice on day 7 and 10, but on day 14 CLA increased lipolysis in both CO- and SO-fed mice (P < 0.001). Expression and activation level of proteins involved in lipolysis and lipogenesis was determined by western blotting and real-time PCR, respectively. No significant differences were detected in protein expression. CO-fed mice had greater fatty acid synthase and stearyl CoA desaturase 1 mRNA expression and less acetyl CoA carboxylase mRNA expression (P < 0.01). Sterol regulatory binding protein 1c was decreased by CLA in CO-fed mice and increased in SO-fed mice (P < 0.05). Malic enzyme expression was increased by CLA (P < 0.001) and CO (P < 0.01). Therefore, CLA-induced lipolysis occurs more rapidly in CO vs SO-fed mice and lipogenesis is decreased in CO-fed mice with CLA supplementation.

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Section: Discussionmentioning

confidence: 90%

Dietary CLA‐induced Lipolysis Is Delayed in Soy Oil‐Fed Mice Compared to Coconut Oil‐Fed Mice

Ippagunta¹,

Angius²,

Sanda³

et al. 2013

Lipids

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Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

Wang,

Liu,

Zhang

et al. 2024

Preprint

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Conjugated linoleic acids (CLAs) can serve as a nutritional intervention to regulate quality, function and fat infiltration in skeletal muscles but the specific cytological mechanisms are still unknown. Here, we applied single-nucleus RNA-sequencing (snRNA-seq) to characterize the cytological mechanism of CLAs regulates fat infiltration in skeletal muscles based on pig models. We investigated the regulatory effects of CLAs on cell populations and molecular characteristics in pig muscles and found CLAs could promote the transformation of fast glycolytic myofibers into slow oxidative myofibers. We also observed three subpopulations including SCD+/DGAT2+, FABP5+/SIAH1+, and PDE4D+/PDE7B+subclusters in adipocytes and CLAs could increase the percentage of SCD+/DGAT2+adipocytes. RNA velocity analysis showed FABP5+/SIAH1+and PDE4D+/PDE7B+adipocytes could differentiate into SCD+/DGAT2+adipocytes. We further verified the differentiated trajectory of mature adipocytes and identified PDE4D+/PDE7B+adipocytes could differentiate into SCD+/DGAT2+and FABP5+/SIAH1+adipocytes by using high IMF content Laiwu pig models. The cell-cell communication analysis identified the interaction network between adipocytes and other subclusters such as fibro/adipogenic progenitors (FAPs). Pseudotemporal trajectory analysis and RNA velocity analysis also showed FAPs could differentiate into PDE4D+/PDE7B+preadipocytes and we discovered the differentiated trajectory of preadipocytes into mature adipocytes. Besides, we found CLAs could promote FAPs differentiate into SCD+/DGAT2+adipocytes via inhibiting c-Jun N-terminal kinase (JNK) signalling pathwayin vitro. This study provides a foundation for regulating fat infiltration in skeletal muscles by using nutritional strategies and provides potential opportunities to serve pig as an animal model to study human fat infiltrated diseases.

show abstract

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

Wang,

Liu,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

Conjugated linoleic acids (CLAs) can serve as a nutritional intervention to regulate quality, function and fat infiltration in skeletal muscles but the specific cytological mechanisms are still unknown. Here, we applied single-nucleus RNA-sequencing (snRNA-seq) to characterize the cytological mechanism of CLAs regulates fat infiltration in skeletal muscles based on pig models. We investigated the regulatory effects of CLAs on cell populations and molecular characteristics in pig muscles and found CLAs could promote the transformation of fast glycolytic myofibers into slow oxidative myofibers. We also observed three subpopulations including SCD + /DGAT2 + , FABP5 + /SIAH1 + , and PDE4D + /PDE7B + subclusters in adipocytes and CLAs could increase the percentage of SCD + /DGAT2 + adipocytes. RNA velocity analysis showed FABP5 + /SIAH1 + and PDE4D + /PDE7B + adipocytes could differentiate into SCD + /DGAT2 + adipocytes. We further verified the differentiated trajectory of mature adipocytes and identified PDE4D + /PDE7B + adipocytes could differentiate into SCD + /DGAT2 + and FABP5 + /SIAH1 + adipocytes by using high IMF content Laiwu pig models. The cell-cell communication analysis identified the interaction network between adipocytes and other subclusters such as fibro/adipogenic progenitors (FAPs). Pseudotemporal trajectory analysis and RNA velocity analysis also showed FAPs could differentiate into PDE4D + /PDE7B + preadipocytes and we discovered the differentiated trajectory of preadipocytes into mature adipocytes. Besides, we found CLAs could promote FAPs differentiate into SCD + /DGAT2 + adipocytes via inhibiting c-Jun N-terminal kinase (JNK) signalling pathway in vitro . This study provides a foundation for regulating fat infiltration in skeletal muscles by using nutritional strategies and provides potential opportunities to serve pig as an animal model to study human fat infiltrated diseases.

show abstract

Mice do not accumulate muscle lipid in response to dietary conjugated linoleic acid1

Cited by 5 publications

References 43 publications

Dietary CLA‐induced Lipolysis Is Delayed in Soy Oil‐Fed Mice Compared to Coconut Oil‐Fed Mice

Dietary CLA‐induced Lipolysis Is Delayed in Soy Oil‐Fed Mice Compared to Coconut Oil‐Fed Mice

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

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