Mice gut microbiota programming by using the infant food profile. The effect on growth, gut microbiota and the immune system

Sánchez-Samper, Elvira Pilar; Gómez-Gallego, Carlos; Andreo-Martínez, Pedro; Salminen, Seppo; Ros, Gaspar

doi:10.1039/c7fo00819h

Cited by 7 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sequencing of the 16S rRNA V3 gene resulted in a total of 281,1112 reads with a minimum and maximum number of reads per sample of 9,411 and 123,598; with 2,250 assigned OTUs. Alpha diversity measured by Shannon index (Figure 5A) significantly decreased (P < 0.0001) in all dietary intervention groups over the four weeks prior to DSS treatment, likely reflecting compositional changes when transitioning from mother's milk to standard mouse chow (34)(35)(36). However, significant differences in alpha diversity were not observed between vitamin B12 deficient, sufficient or supplemented groups of mice after 28 days on respective diets.…”

Section: Altered Vitamin B12 In the Diet Does Not Affect Gut Microbiamentioning

confidence: 96%

Vitamin B12 Deficiency Alters the Gut Microbiota in a Murine Model of Colitis

et al. 2020

View full text Add to dashboard Cite

Purpose: Inflammatory bowel disease (IBD) refers to a spectrum of autoimmune diseases, which result in chronic intestinal inflammation. Previous findings suggest a role for diet, nutrition and dysbiosis of the gut microbiota in both the development and progression of the condition. Vitamin B12 is a key cofactor of methionine synthase and is produced solely by microbes. Previous work links increased levels of homocysteine, a substrate of methionine synthase, MetH, to IBD indicating a potential role for vitamin B12 deficiency in intestinal injury and inflammation. This study assessed the role of vitamin B12 in shaping the gut microbiota and determining responses to intestinal injury using a reproducible murine model of colitis. Methods: The effects of vitamin B12 supplementation and deficiency were assessed in vivo; 3-week-old post-weanling C57Bl/6 mice were divided into three dietary treatment groups: (1) sufficient vitamin B12 (50 mg/Kg), (2) deficient vitamin B12 (0 mg/Kg) and (3) supplemented vitamin B12 (200 mg/Kg) for a period of 4 weeks. Intestinal injury was induced with 2% dextran sodium sulphate (DSS) via drinking water for 5 days. The impact of varying levels of dietary vitamin B12 on gut microbiota composition was assessed using 16S rRNA gene sequencing from fecal samples collected at day 0 and day 28 of the dietary intervention, and 7 days following induction of colitis on day 38, when blood and colonic tissues were also collected. Results: No significant alterations were found in the gut microbiota composition of disease-free animals in response to dietary interventions. By contrast, after DSS-induced colitis, >30 genera were significantly altered in vitamin B12 deficient mice. Altered B12 levels produced no significant effect on composite disease-activity scores; however, administration of a B12 deficient diet resulted in reduced DSS-induced epithelial tissue damage. Conclusions: Vitamin B12 supplementation does not alter the gut microbiota composition under healthy conditions, but does contribute to differential microbial responses and intestinal dysbiosis following the induction of experimental colitis.

show abstract

Section: Altered Vitamin B12 In the Diet Does Not Affect Gut Microbiamentioning

confidence: 96%

Vitamin B12 Deficiency Alters the Gut Microbiota in a Murine Model of Colitis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recent studies have indicated that it is necessary to continue investigating the interaction between GM and the epithelial cells of the GI tract because GM seems to act as an epigenetic regulator of several diseases (Kumar et al, 2014). Thus, dysbiosis or imbalances in the GM can induce intestinal inflammation that is associated with the pathogenesis of several diseases such as obesity, Crohn's disease, irritable bowel syndrome, Obsessive-Compulsive Disorder, Depression, Anxiety, type 2 diabetes mellitus and neurodegenerative diseases including Alzheimer's and ASD (Durbán et al, 2012;Mangiola et al, 2016;Schäffler et al, 2016;Ding et al, 2017;Pradhan et al, 2017;Sanchez-Samper et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

An approach to gut microbiota profile in children with autism spectrum disorder

Andreo-Martínez

García-Martínez

Sánchez-Samper

et al. 2019

Environ Microbiol Rep

View full text Add to dashboard Cite

Summary In recent years, there has been an increase in studies on the implications of gut microbiota (GM) on the behaviour of children with autism spectrum disorders (ASD) due to a dysbiosis in GM that can trigger onset, development or progression of ASD through the microbiota–gut–brain axis. The aim of this study is to carry out a systematic review of articles from the last 6 years that analyse GM in children with ASD compared to GM in control groups. Children with ASD showed a higher abundance of Roseburia and Candida genera, and lower abundance of Dialister, Bilophila, Veillonella, Streptococcus, Coprococcus and Prevotella genera. Those differences can be attributed to factors such as different nationalities, nature of control groups, place where the sample was taken, gastrointestinal (GI) problems or bacterial detection methods. It is still too early to define a specific GM profile of children with ASD, and future studies should focus on homogenizing the characteristics of samples and control groups. Furthermore, new multicentre studies should also focus on the impact of GM on GI physiology, neurophysiology and behaviour of children with ASD, and on performing psychometric analyses of the correlation between the severity of ASD behavioural symptoms and GM profiles.

show abstract

“…However, GI symptoms appear to be due, in part, to dysbiotic GM. In this sense, there is enough scientific evidence regarding the relationship between a dysbiotic GM and the development of diverse chronic diseases such as asthma, intestinal inflammation associated with the pathogenesis of obesity, type 2 diabetes mellitus [12], and other neurodevelopmental disorders [13].…”

Section: Introductionmentioning

confidence: 99%

The Role of Gut Microbiota in Gastrointestinal Symptoms of Children with ASD

Martínez-González

Andreo-Martínez

2019

Medicina

Self Cite

View full text Add to dashboard Cite

Background and objectives: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impaired communication, social interaction disorder, and repetitive behavior. Dysbiotic gut microbiota (GM) could be a contributing factor to the appearance of ASD, as gastrointestinal (GI) symptoms are comorbidities frequently reported in ASD. As there is a lack of reviews about the role played by GM in the GI symptoms of ASD, this work aimed to carry out a systematic review of current studies comparing the GM of children with ASD and GI symptoms with those of healthy controls in the last six years. Materials and Methods: The systematic review was performed following the PRISMA guidelines. The databases chosen were Web of Science, Scopus, PubMed, and PsycINFO, and the keywords were (gut* OR intestine* OR bowel* OR gastrointestinal*) AND (microbiota* OR microflora* OR bacteria* OR microbiome* OR flora* OR bacterial* OR bacteria* OR microorganism* OR feces* OR stool*) AND (autistic* OR autism* OR ASD*). Results: A total of 16 articles were included. Ten articles performed correlations analysis between GI symptoms and ASD. Among those 10 articles, 7 found differences between the GI symptoms present in children with ASD and healthy controls. The most common GI symptom was constipation. Among the seven articles that found differences, three performed correlations analysis between GI symptoms and gut microbe abundance. Candida, Prevotella, Streptococcus, and Veillonella showed higher and lower abundance, respectively, in children with ASD and GI symptoms in more than one article. Bacteroidetes, Firmicutes, Actinomyces, Dorea, Lactobacillus, Faecalibacterium prausnitzii, and Bacteroidetes/Firmicutes ratios showed abundance discrepancies. Conclusions: It is still too early to draw a conclusion about the gut microbes involved in GI symptoms of ASD. Future research should consider the relationship between ASD behavior, GM, and GI symptoms in a multidisciplinary way and homogenize sample characteristics.

show abstract

Mice gut microbiota programming by using the infant food profile. The effect on growth, gut microbiota and the immune system

Cited by 7 publications

References 48 publications

Vitamin B12 Deficiency Alters the Gut Microbiota in a Murine Model of Colitis

Vitamin B12 Deficiency Alters the Gut Microbiota in a Murine Model of Colitis

An approach to gut microbiota profile in children with autism spectrum disorder

The Role of Gut Microbiota in Gastrointestinal Symptoms of Children with ASD

Contact Info

Product

Resources

About