Mice Vaccination with High Hydrostatic Pressure-Inactivated H3N8 Virus Protects Against Experimental Avian Flu

Barroso, Shana Priscila Coutinho; Nico, Dirlei; Gomes, Daniel; Santos, Ana C. Vicente; Couceiro, J. N. S. S.; Palatnik-de-Sousa, Clarisa B.; Silva, Jerson L.; Oliveira, Andréa C.

doi:10.1016/j.provac.2012.04.014

Cited by 7 publications

(13 citation statements)

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“…HHP has the potential to cause viral inactivation without drastically affecting viral immunogenic properties or destroying structural epitopes [10,11,19,22]. This interesting finding highlights the potential application of this tool to prepare whole viral vaccines in a simple, fast, and inexpensive way.…”

Section: Introductionmentioning

confidence: 99%

Full Inactivation of Human Influenza Virus by High Hydrostatic Pressure Preserves Virus Structure and Membrane Fusion While Conferring Protection to Mice against Infection

et al. 2013

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Whole inactivated vaccines (WIVs) possess greater immunogenicity than split or subunit vaccines, and recent studies have demonstrated that WIVs with preserved fusogenic activity are more protective than non-fusogenic WIVs. In this work, we describe the inactivation of human influenza virus X-31 by high hydrostatic pressure (HHP) and analyze the effects on the structure by spectroscopic measurements, light scattering, and electron microscopy. We also investigated the effects of HHP on the glycoprotein activity and fusogenic activity of the viral particles. The electron microscopy data showed pore formation on the viral envelope, but the general morphology was preserved, and small variations were seen in the particle structure. The activity of hemagglutinin (HA) during the process of binding and fusion was affected in a time-dependent manner, but neuraminidase (NA) activity was not affected. Infectious activity ceased after 3 hours of pressurization, and mice were protected from infection after being vaccinated. Our results revealed full viral inactivation with overall preservation of viral structure and maintenance of fusogenic activity, thereby conferring protection against infection. A strong response consisting of serum immunoglobulin IgG1, IgG2a, and serum and mucosal IgA was also detected after vaccination. Thus, our data strongly suggest that applying hydrostatic pressure may be an effective method for developing new vaccines against influenza A as well as other viruses.

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Section: Introductionmentioning

confidence: 99%

Full Inactivation of Human Influenza Virus by High Hydrostatic Pressure Preserves Virus Structure and Membrane Fusion While Conferring Protection to Mice against Infection

et al. 2013

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“…In the influenza virus, this change is usually referred to as the "spring-loaded" model and is trigged by low pH, which indicates a mechanism in which the fusion peptide region is inserted into the target membrane in an early step of the fusion process (Skehel and Wiley 2000). We observed that subjecting the influenza virus to pressure exposes hydrophobic domains even in neutral pH (Gaspar et al 2002;Barroso et al 2012;Dumard et al 2013) (Fig. 15.1).…”

Section: Pressure Effects On Viral Particlesmentioning

confidence: 84%

“…Therefore, HHP is a technology for the production of inactivated vaccines that are free of chemicals, safe and capable of inducing strong humoral and cellular immune responses (Silva et al 1992;Tian et al 1999;Barroso et al 2012;Dumard et al 2013). Therefore, HHP is a technology for the production of inactivated vaccines that are free of chemicals, safe and capable of inducing strong humoral and cellular immune responses (Silva et al 1992;Tian et al 1999;Barroso et al 2012;Dumard et al 2013).…”

Section: Why Would a Pressurized Vaccine Results In A Bettermentioning

confidence: 99%

“…After the challenge, none of the vaccinated animals developed clinical signs compared with the control group (Barroso et al 2012). After immunization, there was an increase of IgG1 and IgG2a in the serum and IgA in the nasal lavage.…”

Section: Immune Response To Pressurized Virusmentioning

confidence: 86%

“…Several studies have demonstrated that pressure can result in virus inactivation while preserving immunogenic properties (Silva et al 1992;Oliveira et al 1999;Tian et al 2000;Barroso et al 2012;Dumard et al 2013). The ability of pressure to inactivate viruses, prions and bacteria has been evaluated in relation to the applications of vaccine development and virus sterilization.…”

Section: Summary and Concluding Remarksmentioning

confidence: 99%

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High Pressure Bioscience

Akasaka¹,

Matsuki²

2015

Subcellular Biochemistry

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The book series SUBCELLULAR BIOCHEMISTRY is a renowned and well recognized forum for disseminating advances of emerging topics in Cell Biology and related subjects. All volumes are edited by established scientists and the individual chapters are written by experts on the relevant topic. The individual chapters of each volume are fully citable and indexed in Medline/Pubmed to ensure maximum visibility of the work.

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Pressure-Inactivated Virus: A Promising Alternative for Vaccine Production

Silva

Barroso

Mendes

et al. 2015

Subcellular Biochemistry

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In recent years, many applications in diverse scientific fields with various purposes have examined pressure as a thermodynamic parameter. Pressure studies on viruses have direct biotechnological applications. Currently, most studies that involve viral inactivation by HHP are found in the area of food engineering and focus on the inactivation of foodborne viruses. Nevertheless, studies of viral inactivation for other purposes have also been conducted. HHP has been shown to be efficient in the inactivation of many viruses of clinical importance and the use of HHP approach has been proposed for the development of animal and human vaccines. Several studies have demonstrated that pressure can result in virus inactivation while preserving immunogenic properties. Viruses contain several components that can be susceptible to the effects of pressure. HHP has been a valuable tool for assessing viral structure function relationships because the viral structure is highly dependent on protein-protein interactions. In the case of small icosahedral viruses, incremental increases in pressure produce a progressive decrease in the folding structure when moving from assembled capsids to ribonucleoprotein intermediates (in RNA viruses), free dissociated units (dimers and/or monomers) and denatured monomers. High pressure inactivates enveloped viruses by trapping their particles in a fusion-like intermediate state. The fusogenic state, which is characterized by a smaller viral volume, is the final conformation promoted by HHP, in contrast with the metastable native state, which is characterized by a larger volume. The combined effects of high pressure with other factors, such as low or subzero temperature, pH and agents in sub-denaturing conditions (urea), have been a formidable tool in the assessment of the component's structure, as well as pathogen inactivation. HHP is a technology for the production of inactivated vaccines that are free of chemicals, safe and capable of inducing strong humoral and cellular immune responses. Here we present a current overview about the pressure-induced viral inactivation and the production of inactivated viral vaccines.

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Mice Vaccination with High Hydrostatic Pressure-Inactivated H3N8 Virus Protects Against Experimental Avian Flu

Cited by 7 publications

References 19 publications

Full Inactivation of Human Influenza Virus by High Hydrostatic Pressure Preserves Virus Structure and Membrane Fusion While Conferring Protection to Mice against Infection

Full Inactivation of Human Influenza Virus by High Hydrostatic Pressure Preserves Virus Structure and Membrane Fusion While Conferring Protection to Mice against Infection

High Pressure Bioscience

Pressure-Inactivated Virus: A Promising Alternative for Vaccine Production

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