Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances

Analysis of antibiotics by capillary electrophoresis

“…Although the separation was optimized in a phosphate buffer, the authors concluded that better separations could be obtained in either a 40 mM citrate buffer, pH 5.5, or a 260 mM MES buffer, pH 6.21 [11]. Two SDS micellar systems were utilized for the separation of cefalexin and ten of its related substances [12]. Buffer conditions were optimized in both acetate and phosphate buffers; the 50 mM SDS, 50 mM acetate buffer, pH 5.25, was chosen for further use because the current was lower and the analysis time shorter than in phosphate.…”

“…Buffer conditions were optimized in both acetate and phosphate buffers; the 50 mM SDS, 50 mM acetate buffer, pH 5.25, was chosen for further use because the current was lower and the analysis time shorter than in phosphate. Under assay conditions, a 4 s injection was used for method validation, to produce a linear range from 0.25 to 1.50 mg/mL with an r 2 of 0.998 [12]. The authors noted that an 8 s injection of a 2 mg/mL solution of cefalexin was required to observe related substances in commercial samples [12].…”

Application ofl-cysteine capped core–shell CdTe/ZnS nanoparticles as a fluorescence probe for cephalexin

“…Up to now, there have been many literature sources reported on the methods for determination of cephalexin, including high-performance liquid chromatography (HPLC), 7,8 liquid chromatography (LC), 9 ow injection analysis, 10 ow-through immunoanalysis, 11 electrochemical methods, 12,13 and micellar electrokinetic capillary chromatography (MEKC). 14 Despite the sensitivities of these methods, they have certain drawbacks like long time consumption, cumbersome operation and high cost.…”