Micellar Electrokinetic Capillary Chromatography of Pungent Compounds Using Simultaneous On-line Ultraviolet and Electrochemical Detection

Khaled, Maha Y.; Anderson, Mark R.; McNair, Harold M.

doi:10.1093/chromsci/31.7.259

Cited by 13 publications

(4 citation statements)

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“…Analysis of capsaicinoids has been performed by using spectrophotometric [19,20], gas chromatographic [21], micellar electrokinetic capillary chromatographic [22][23][24], high performance liquid chromatographic [25][26][27][28][29][30], liquid chromatographic-mass spectrometric [31][32][33][34][35] and enzyme immunoassays [36] procedures. The present method involves a simple and easy-to-perform HPLC separation coupled with fluorescent detection providing high sensitivity and selectivity due to the strong fluorescence of the hydroxymethoxybenzyl group of capsaicinoids [27].…”

Section: Introductionmentioning

confidence: 99%

A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat

Kuźma

Fodor

Maász

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat

Kuźma

Fodor

Maász

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…• micellar electrokinetic capillary chromatography (Khaled et al 1993); • mass spectroscopy and nuclear magnetic resonance (NMR) spectroscopy (Bennett and Kirby 1968); and • infrared spectroscopy (Grasselli 1975).…”

Section: Methodsmentioning

confidence: 99%

Final Report on the Safety Assessment of Capsicum Annuum Extract, Capsicum Annuum Fruit Extract, Capsicum Annuum Resin, Capsicum Annuum Fruit Powder, Capsicum Frutescens Fruit, Capsicum Frutescens Fruit Extract, Capsicum Frutescens Resin, and Capsaicin1

2007

Int J Toxicol

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Capsicum-derived ingredients function as skin-conditioning agents--miscellaneous, external analgesics, flavoring agents, or fragrance components in cosmetics. These ingredients are used in 19 cosmetic products at concentrations as high as 5%. Cosmetic-grade material may be extracted using hexane, ethanol, or vegetable oil and contain the full range of phytocompounds that are found in the Capsicum annuum or Capsicum frutescens plant (aka red chiles), including Capsaicin. Aflatoxin and N-nitroso compounds (N-nitrosodimethylamine and N-nitrosopyrrolidine) have been detected as contaminants. The ultraviolet (UV) absorption spectrum for Capsicum Annuum Fruit Extract indicates a small peak at approximately 275 nm, and a gradual increase in absorbance, beginning at approximately 400 nm. Capsicum and paprika are generally recognized as safe by the U.S. Food and Drug Administration for use in food. Hexane, chloroform, and ethyl acetate extracts of Capsicum Frutescens Fruit at 200 mg/kg resulted in death of all mice. In a short-term inhalation toxicity study using rats, no difference was found between vehicle control and a 7% Capsicum Oleoresin solution. In a 4-week feeding study, red chilli (Capsicum annuum) in the diet at concentrations up to 10% was relatively nontoxic in groups of male mice. In an 8-week feeding study using rats, intestinal exfoliation, cytoplasmic fatty vacuolation and centrilobular necrosis of hepatocytes, and aggregation of lymphocytes in the portal areas were seen at 10% Capsicum Frutescens Fruit, but not 2%. Rats fed 0.5 g/kg day-1 crude Capsicum Fruit Extract for 60 days exhibited no significant gross pathology at necropsy, but slight hyperemia of the liver and reddening of the gastric mucosa were observed. Weanling rats fed basal diets supplemented with whole red pepper at concentrations up to 5.0% for up to 8 weeks had no pathology of the large intestines, livers, and kidneys, but destruction of the taste buds and keratinization and erosion of the gastrointestinal (GI) tract were noted in groups fed 0.5% to 5.0% red pepper. The results of 9-and 12-month extension of this study showed normal large intestines and kidneys. In rabbits fed Capsicum Annuum Powder at 5 mg/kg day-1 in the diet daily for 12 months damage to the liver and spleen was noted. A rabbit skin irritation test of Capsicum Annuum Fruit Extract at concentrations ranging from 0.1% to 1.0% produced no irritation, but Capsicum Frutescens Fruit Extract induced concentration-dependent (at 25 to 500 microg/ml) cytotoxicity in a human buccal mucosa fibroblast cell line. An ethanol extract of red chili was mutagenic in Salmonella typhimurium TA98, but not in TA100, or in Escherichia coli. Other genotoxicity assays gave a similar pattern of mixed results. Adenocarcinoma of the abdomen was observed in 7/20 mice fed 100 mg red chilies per day for 12 months; no tumors were seen in control animals. Neoplastic changes in the liver and intestinal tumors were observed in rats fed red chili powder at 80 mg/kg day-1 for 30 days, intestinal and colo...

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“…The effect of applied voltage on separations can improve peak shape and reduce separation time dramatically [35]. Increasing the applied voltage in CE can also cause Joule heating of the capillary, resulting in bubble formation in the capillary or even breakage of the capillary.…”

Section: Separation Propertiesmentioning

confidence: 99%

“…Absolute detection limits (pg) were calculated by multiplying the volume injected by the concentration of the dye. The volume of each dye injected can be determined using the equation [35]:…”

Section: Detection Limitsmentioning

confidence: 99%

Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Detection for Separation of Red and Yellow Historical Dyes

2013

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Abstract:In this study, the separation parameters in micellar electrokinetic chromatography (MEKC)-laser-induced fluorescence (LIF) were optimized for the separation of red and yellow historical dyes in 20 mM borate buffer with 20 mM sodium dodecyl sulfate (SDS). Separation conditions were optimized by changing pH, organic modifier (methanol and acetonitrile) concentrations and applied voltage. The mixtures of dyes used in this study included four anthraquinone dyes (alizarin, purpurin, emodin and carmine) and six flavonoid dyes (luteolin, apigenin, kaempferol, quercetin, morin and myricetin). For this work, dyes were introduced electro-kinetically (10 kV for 5 s) into a 50 cm capillary (10 µm id) and separated using a running potential of 18, 20, 22 and 25 kV. Absolute limits of detection for most of these dyes was less than 1 pg. For dyes such as alizarin, improved detection limits were achieved at pH = 9.24; however dyes such as purpurin had significantly improved detection limits at pH = 8.0. The successful extraction and identification of a number of dyes in plants and textiles samples is also described.

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Micellar Electrokinetic Capillary Chromatography of Pungent Compounds Using Simultaneous On-line Ultraviolet and Electrochemical Detection

Cited by 13 publications

References 0 publications

A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat

A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat

Final Report on the Safety Assessment of Capsicum Annuum Extract, Capsicum Annuum Fruit Extract, Capsicum Annuum Resin, Capsicum Annuum Fruit Powder, Capsicum Frutescens Fruit, Capsicum Frutescens Fruit Extract, Capsicum Frutescens Resin, and Capsaicin1

Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Detection for Separation of Red and Yellow Historical Dyes

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