Micellar electrokinetic chromatography and laser induced fluorescence detection of botulinum neurotoxin type A activity using a dual-labelled substrate

Wei, Hongping; Kang, Yong-Koo; Zhang, Zhiping; Cui, Zongqiang; Zhou, Ya‐Feng; Zhang, Xian-En

doi:10.1080/03067310802065208

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…In addition, there are various other techniques emerging as potential BoNT detection platforms. For example, surface plasmon resonance [ 137 , 138 , 139 ], rapid detection by transistors with gates modified by anti-toxin antibodies by change in drain-source current upon BoNT binding [ 140 ], liquid chromatography coupled to mass spectrometry [ 141 ], capillary electrophoresis [ 142 ], micro¬mechanosensor able to detect synaptobrevin molecule cleavage by BoNT/B [ 143 ] or time resolved fluorescence assay [ 144 ]. Many of these techniques show promise but rely on expensive instrument¬ation and/or have not been sufficiently tested.…”

Section: Emerging In Vitro Assays and Technologiesmentioning

confidence: 99%

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Čapek

Dickerson

2010

Toxins

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Sensitive and rapid detection of botulinum neurotoxins (BoNTs), the most poisonous substances known to date, is essential for studies of medical applications of BoNTs and detection of poisoned food, as well as for response to potential bioterrorist threats. Currently, the most common method of BoNT detection is the mouse bioassay. While this assay is sensitive, it is slow, quite expensive, has limited throughput and requires sacrificing animals. Herein, we discuss and compare recently developed alternative in vitro detection methods and assess their ability to supplement or replace the mouse bioassay in the analysis of complex matrix samples.

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Section: Emerging In Vitro Assays and Technologiesmentioning

confidence: 99%

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Čapek

Dickerson

2010

Toxins

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“…The problem with these techniques as detection platforms, is that they often require expensive instrumentation or have simply not been tested across a wide enough set of parameters. These factors have led to other detection methods stagnating and not progressing in advancement such as capillary electrophoresis [127] and even a micro-mechanical sensor able to detect synaptobrevin molecule cleavage [128].…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Botulinum Neurotoxins—A Review

Hobbs

Thomas

Halliwell

et al. 2019

Toxins

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A toxin is a poisonous substance produced within living cells or organisms. One of the most potent groups of toxins currently known are the Botulinum Neurotoxins (BoNTs). These are so deadly that as little as 62 ng could kill an average human; to put this into context that is approximately 200,000 × less than the weight of a grain of sand. The extreme toxicity of BoNTs leads to the need for methods of determining their concentration at very low levels of sensitivity. Currently the mouse bioassay is the most widely used detection method monitoring the activity of the toxin; however, this assay is not only lengthy, it also has both cost and ethical issues due to the use of live animals. This review focuses on detection methods both existing and emerging that remove the need for the use of animals and will look at three areas; speed of detection, sensitivity of detection and finally cost. The assays will have wide reaching interest, ranging from the pharmaceutical/clinical industry for production quality management or as a point of care sensor in suspected cases of botulism, the food industry as a quality control measure, to the military, detecting BoNT that has been potentially used as a bio warfare agent.

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Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2009

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The use of capillary electromigration methods to analyze foods and food components is reviewed in this work. Papers that were published during the period April 2007 to March 2009 are included following the previous review by García-Cañas and Cifuentes (Electrophoresis, 2008, 29, 294-309). These works include the analysis of amino acids, biogenic amines, peptides, proteins, DNAs, carbohydrates, phenols, polyphenols, pigments, toxins, pesticides, vitamins, additives, small organic and inorganic ions and other compounds found in foods and beverages, as well as those applications of CE for monitoring food interactions and food processing. The use of microchips, CE-MS, chiral-CE as well as other foreseen trends in food analysis are also discussed including their possibilities in the very new field of Foodomics.

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Micellar electrokinetic chromatography and laser induced fluorescence detection of botulinum neurotoxin type A activity using a dual-labelled substrate

Cited by 8 publications

References 29 publications

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Rapid Detection of Botulinum Neurotoxins—A Review

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

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