Micellar liquid chromatography determination of some biogenic amines with electrochemical detection

Bose, Devasish; Durgbanshi, Abhi̧lasha; Capella‐Peiró, María‐Elisa; Gil-Agustı́, Mayte; Esteve‐Romero, Josep; Carda‐Broch, Samuel

doi:10.1016/j.jpba.2004.06.026

Cited by 23 publications

(8 citation statements)

References 30 publications

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“…A variety of methods have been developed to measure serotonin including spectrophotometry [21,22], LCfluorometry [23,24], enzyme immunoassay, and LCelectrochemical detection [25,26]. LC-mass spectrometric methods is however the method of choice as tandem LC-MS methods provide increased selectivity while reducing the need for dependence on chromatographic separation [27][28][29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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Serotonin is a major neurotransmitter and affects various functions both in the brain and in the rest of the body. It has been demonstrated that altered serotinergic function is implicated in various psychiatric disorders including depression and schizophrenia. Serotonin has also been implicated along with dopamine in attention deficithyperkinetic disorder (AD-HKD). This study provides a versatile validated method for the analysis of serotonin, hydroxyindole acetic acid and dopamine in urine using LC-MS/MS. This method was then used to quantify these analytes in a test group of 17 children diagnosed with severe AD-HKD. This group was compared to a matched control group to investigate the possibility that one of these compounds may be a potential biomarker for this condition. The developed method provided good linear calibration curves for the multiplex assay of analytes in urine (0.05-3.27 nmol/L; R 2 ≥0.9977). Acceptable inter-day repeatability was achieved for all analytes with RSD values (n=9) ranging from 1.1% to 9.3% over a concentration range of 0.11-3.27 μmol/L in urine. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 8.8-18.2 nmol/L and the LOQs of 29.4-55.7 nmol/L for analytes in urine. Recoveries were in the ranges of 98-104%, 100-106% and 91-107% for serotonin, 5-HIAA and dopamine, respectively. An appropriate sample clean-up procedure for urine was developed to ensure efficient recovery and reproducibility on analysis. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. Confirmatory analysis was carried out on a linear trap quadrupole-Orbitrap mass spectrometer to obtain high mass accuracy data of the target analytes in the clinical samples.

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Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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“…The aim of the research was the development of a method based on micellar liquid chromatography (MLC) to determine MBZ in milk and other dairy products such as curd, butter, and cheese to check the absence of residues in the food and ensure consumer protection, and farming waste (urine and dung), since its presence in excretions is proof the drug has been administered to the bovine animal. MLC allows direct injection of samples into the chromatographic system without any pretreatment; using sodium dodecyl sulfate (SDS) as a surfactant in mobile phase makes the method rapid, sensitive, cost effective, and eco-friendly, as well as serves as an alternative to conventional HPLC system [20][21][22][23]. These are some of the main reasons behind selecting MLC to carry out the present research work.…”

Section: Introductionmentioning

confidence: 99%

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

et al. 2020

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Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)-6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25-50 ppm), sensitivity (limit of detection 0.1-0.2 ppm; limit of quantification, 0.3-0.6 ppm), trueness (92.5-102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.

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“…As results, they are less toxic, less flammable, more stable and environmental-friendly and relatively inexpensive if compared to hydroorganic mobile phases. 35 MLC has been useful in the determination of a large amount of drugs, as ergot alkaloids, 36 antibiotics, 37 disulfiram, 38 antidepressants, 39 antianginals, 40 diuretics, 41 benzodiazepines, phenethylamines, antihistamines, vitamins and corticosteroids 42 in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Tamoxifen in Pharmaceutical Formulations Using Micellar Liquid Chromatography

2014

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This paper describes a micellar liquid chromatographic method used to analyze tamoxifen (TAMO) in pharmaceutical formulations, while focusing in its interesting features. Solid samples were solved in a micellar solution, irradiated at 254 nm, filtered and injected. Extraction steps were avoided and thus expediting the procedure. Tamoxifen was resolved in <5 min, using a mobile phase containing 0.15 M sodium dodecyl sulfate-7% pentanol at pH 3, running at 1.5 mL/min under an isocratic mode at 40 C through a C18 column. Detection was achieved by fluorescence by excitation at 260 nm and emission at 380 nm. The validation was performed following the requirements of the International Conference on Harmonization (ICH) Tripartite Guidelines in terms of: specificity, sensitivity, calibration range (0.2 -20 mg/L), accuracy (98.8 -101.7%), precision (<1.5%) and robustness (<6.2%). The method was applied to quantify TAMO in TAMO citrate tablets supplied in Spain, and was found appropriate for the quality control of TAMO formulations.

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Micellar liquid chromatography determination of some biogenic amines with electrochemical detection

Cited by 23 publications

References 30 publications

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

Quantification of Tamoxifen in Pharmaceutical Formulations Using Micellar Liquid Chromatography

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