Micelle mediated extraction of fatty acids from microalgae cultures: Implementation for outdoor cultivation

Glembin, Philipp; Racheva, Ralena; Kerner, Martin; Смирнова, Ирина

doi:10.1016/j.seppur.2014.07.057

Cited by 28 publications

(14 citation statements)

References 30 publications

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“…In these cases, the surfactant is used to extract target molecules from the cell media where the composition of the cultivation solution is not preliminary changed. 7,8 Moreover, different salts and organic compounds are present in the cultivation broths for biomass productions. For instance, yeast and microalgae are cultivated under salt stress in order to increase the algae carotenoids productivity or the yeast enzyme activity.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies show the applicability of cloud point extraction using nonionic surfactants from yeast and algae cultivation broths. In these cases, the surfactant is used to extract target molecules from the cell media where the composition of the cultivation solution is not preliminary changed. , Moreover, different salts and organic compounds are present in the cultivation broths for biomass productions. For instance, yeast and microalgae are cultivated under salt stress in order to increase the algae carotenoids productivity or the yeast enzyme activity. , Therefore, the knowledge of the influence of salts present in the cultivation broth on the CPT is essential for the design of an extraction process.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Inorganic Salts on the Phase Equilibrium of Triton X-114 Aqueous Two-Phase Systems

et al. 2016

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In this work, we studied the liquid–liquid equilibrium of ternary systems containing nonionic surfactant Triton X-114, inorganic salts, and water. The salt impact of five different sodium salts (NaCl, NaBr, NaI, Na2SO4, and Na2HPO4) and four different chlorides (NaCl, KCl, LiCl, NH4Cl) was investigated. The influence of the inorganic additives was studied at 30 °C because at this temperature all prepared mixtures faced a separation above the cloud point temperature (CPT) in micellar (surfactant-rich) and aqueous (surfactant-lean) phase. The salting-out ability of the cations follows the series Na+ > K+ > NH4 + > Li+. We observed a more pronounced effect among the anions, where the salting-out effect decreased in the order HPO4 2– ≈ SO4 2– > Cl– > Br– > I–. All salts, except NaI, lead to an increase in the Triton X-114 concentration in the micellar phase and to a decrease of the surfactant fraction in the aqueous phase. Inorganic salts also distributed unevenly between both phases and, thus, accumulated in the aqueous phase. In the case of stronger salting-out kosmotropic anions (HPO4 2–, SO4 2–), the salt load of water in the aqueous phase was higher compared to the weaker agents Br– and I–.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Inorganic Salts on the Phase Equilibrium of Triton X-114 Aqueous Two-Phase Systems

et al. 2016

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“…There is a serious limitation of micellization models that restricts substantially their use in engineering applications. For micellar separation, particularly important is knowledge of the details of the local composition of the micelle including the knowledge of where and how the target organic substance is incorporated in the micelle interior. Unfortunately, the existing aggregation models do not provide such a detailed picture.…”

Section: Micelles In Solutions Of Classical Surfactants and Ionic Liqmentioning

confidence: 99%

Molecular thermodynamics of soft self-assembling structures for engineering applications

Victorov

2015

J. Chem. Technol. Biotechnol.

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Controlling self‐assembly is a key issue in many applications such as encapsulation of drugs, micelle separation, and fabrication of nanoporous materials. For providing guidance to control self‐assembly, a reliable prognosis of aggregation behavior is indispensable. Molecular thermodynamic models have been developed for different types of soft mesoscale structures formed by aggregating chainlike amphiphilic molecules. Examples include nonionic and ionic copolymer gels swelling in selective solvents, surfactant micellar solutions and amphiphilic membranes. Though rather different in chemical nature and applications, these systems are all characterized by self‐assembly into soft mesoscale structures that are sensitive to external conditions or applied stimuli. The models predict a number of thermodynamic and structural characteristics (equilibrium size and stability of different morphologies, equilibrium swelling, elastic properties, solute partitioning, etc.) in terms of several adjustable parameters and molecular characteristics of components. Consideration of nanoscale morphology gives rise to interesting structure–property relations reflected by relatively simple models. New findings help estimate how variations of controllable factors such as pH, salinity and additives affect self‐assembly patterns and aggregate properties. Recent advances in the development of molecular thermodynamic aggregation models, factors restricting implementation of these models and trends in the field are discussed. © 2015 Society of Chemical Industry

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“…Among the downstream process techniques suitable for separation and purification of biomolecules, downstream processes applying aqueous two-phase systems (ATPSs) could be an alternative to conventional chromatographic approaches. 17 , 18 , 21 − 23 As coacervation systems, they mostly provide an aqueous environment and mild operational conditions, allowing the molecule to retain its native conformation, which makes them attractive alternatives also for enzymatic synthesis in flow. 24 Recently, aqueous micellar two-phase systems (AMTPSs) as a special version of ATPSs have been introduced.…”

Section: Introductionmentioning

confidence: 99%

Development of a Microfluidic Platform for R-Phycoerythrin Purification Using an Aqueous Micellar Two-Phase System

Seručnik

Vicente

Brečko

et al. 2020

ACS Sustainable Chem. Eng.

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Temperature-dependent aqueous micellar two-phase systems (AMTPSs) have recently been gaining attention in the isolation of high-added-value biomolecules from their natural sources. Despite their sustainability, aqueous two-phase systems, and particularly AMTPSs, have not been extensively applied in the industry, which might be changed by applying process integration and continuous manufacturing. Here, we report for the first time on an integrated microfluidic platform for fast and low-material-consuming development of continuous protein purification using an AMTPS. A system comprised of a microchannel incubated at high temperature, enabling instantaneous triggering of a two-phase system formation, and a microsettler, allowing complete phase separation at the outlets, is reported here. The separation of phycobiliproteins and particularly the purification of R-phycoerythrin from the contaminant proteins present in the aqueous crude extract obtained from fresh cells of Gracilaria gracilis were thereby achieved. The results from the developed microfluidic system revealed that the fractionation performance was maintained while reducing the processing time more than 20-fold when compared with the conventional lab-scale batch process. Furthermore, the integration of a miniaturized ultrafiltration module resulted in the complete removal of the surfactant from the bottom phase containing R-phycoerythrin, as well as in nearly twofold target protein concentration. The process setup successfully exploits the benefits of process intensification along with the integration of various downstream processes. Further transfer to a meso-scale integrated system would make such a system appropriate for the separation and purification of biomolecules with high commercial interest.

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Micelle mediated extraction of fatty acids from microalgae cultures: Implementation for outdoor cultivation

Cited by 28 publications

References 30 publications

Influence of Inorganic Salts on the Phase Equilibrium of Triton X-114 Aqueous Two-Phase Systems

Influence of Inorganic Salts on the Phase Equilibrium of Triton X-114 Aqueous Two-Phase Systems

Molecular thermodynamics of soft self-assembling structures for engineering applications

Development of a Microfluidic Platform for R-Phycoerythrin Purification Using an Aqueous Micellar Two-Phase System

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