Miconazole: An effective antifungal agent for plant tissue culture

Tynan, J. L.; Conner, A. J.; Macknight, Richard C.; Poulter, Russell T. M.

doi:10.1007/bf00042292

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…When developing the MSTT formulation, we identified terbinafine as a candidate antifungal reagent from an earlier study that investigated squalene metabolism in Arabidopsis (Laranjeira et al ). We also tested miconazole (20 mg l −1 ) on the basis that it had been used to prevent fungal overgrowth in explant tissue culture of a number of crop species (Tynan et al ), but miconazole was 100% lethal to all of our Arabidopsis lines at this concentration (data not shown). It is possible that miconazole would be useful at lower concentrations but we did not pursue this further given our immediate success with terbinafine.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial solid media for screening non‐sterile Arabidopsis thaliana seeds

Behrendorff

Borràs-Gas

Pribil

2020

Physiologia Plantarum

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Stable genetic transformation of plants is a low‐efficiency process, and identification of positive transformants usually relies on screening for expression of a co‐transformed marker gene. Often this involves germinating seeds on solid media containing a selection reagent. Germination on solid media requires surface sterilization of seeds and careful aseptic technique to prevent microbial contamination, but surface sterilization techniques are time consuming and can cause seed mortality if not performed carefully. We developed an antimicrobial cocktail that can be added to solid media to inhibit bacterial and fungal growth without impairing germination, allowing us to bypass the surface sterilization step. Adding a combination of terbinafine (1 μM) and timentin (200 mg l−1) to Murashige and Skoog agar delayed the onset of observable microbial growth and did not affect germination of non‐sterile seeds from 10 different wild‐type and mutant Arabidopsis thaliana accessions. We named this antimicrobial solid medium “MSTT agar”. Seedlings sown in non‐sterile conditions could be maintained on MSTT agar for up to a week without observable contamination. This medium was compatible with rapid screening methods for hygromycin B, phosphinothricin (BASTA) and nourseothricin resistance genes, meaning that positive transformants can be identified from non‐sterile seeds in as little as 4 days after stratification, and transferred to soil before the onset of visible microbial contamination. By using MSTT agar we were able to select genetic transformants on solid media without seed surface sterilization, eliminating a tedious and time‐consuming step.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial solid media for screening non‐sterile Arabidopsis thaliana seeds

Behrendorff

Borràs-Gas

Pribil

2020

Physiologia Plantarum

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“…Tynan et al found no significant phytotoxicity of miconazole (5-20 mg/L) on shoot cultures of nine plant species. Callus and hairy root cultures from five species were found to be more sensitive to miconazole than shoot cultures, although all these cultures showed a positive growth rate [7].…”

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confidence: 96%

Effect of Miconazole and Terbinafine on Artemisinin Content of Shooty Teratoma of Artemisia annua

Jain

Dixit

2010

Natural Product Communications

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The effects produced by the addition of sterol synthesis inhibitors on the artemisinin content of the transgenic organ culture (A. tumefaciens ATCC 33970 or 15955) of Artemisia annua are presented. The transgenic tissue produced 3-4 fold higher levels of artemisinin 0.84% (56.3 mg/L) within a short culture period compared with field grown plants (0.23%). The addition of the sterol synthesis inhibitors, miconazole and terbinafine, to these transgenic cultures resulted in enhanced artemisinin content up to 1.15% and 1.44%, respectively. Further enhancement of artemisinin content was achieved by varying the addition time of the sterol synthesis inhibitor to the cultures. The best artemisinin content (2.62%) was observed after terbinafine (10 mg/L) addition on the sixteenth day of the culture period.

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