Questions regarding the application of pre-emergence herbicides for control weeds in eucalyptus are noteworthy due to the impact of this practice on the environment, particularly on soil microbial activity and beneficial microorganisms such as mycorrhizal fungi and phosphate solubilizing microorganisms. The aim of this study was to evaluate the impact of applying herbicides sulfentrazone, isoxaflutole and oxyfluorfen on mycorrhizal colonization, microbial biomass and microbial activity of two forest soils cultivated with eucalyptus. The experiment was conducted in a greenhouse using two soils (clayey and sandy loam) distributed in 12 dm 3 pots. The 4x3 factorial design was used, with three herbicides (sulfentrazone, oxyfluorfen, isoxaflutole) over the untreated control and three assessments (5, 20 and 70 days after application (DAA)), in a completely randomized design with four replications. After herbicide application, seedlings of the hybrid Eucalyptus grandis x Eucalyptus urophylla were transplanted. We evaluated the microbial biomass, respiration rate, metabolic quotient and the potential of solubilization of inorganic phosphate at 5, 20 and 70 DAA. At 70 DAA verified the percentage of roots colonized by mycorrhizal fungi and spore viability in the soil. The sulfentrazone, isoxaflutole and oxyfluorfen as well as the time of application affected the microbiological indicators differently. In the sandy loam soil sulfentrazone was more harmful to microbial biomass, mycorrhizal colonization and microorganisms solubilizing inorganic phosphate. However, in clayey soil, the application of the three herbicides did not affect microbial biomass, but the root colonization of eucalyptus by mycorrhizal fungi and the potential solubilization of inorganic phosphate were reduced. The sulfentrazone caused an increase in the number of non-viable spores of mycorrhizal fungi in the soil.Key words: herbicides, Eucalyptus grandis x Eucalyptus urophylla, mycorrhiza, phosphate solubilization, microbial biomass.Impacto de sulfentrazona, isoxaflutol e oxyfluorfem sobre a microbiota de dois solos florestais
ResumoQuestões referentes à aplicação de herbicidas em pré-emergência das plantas daninhas na cultura do eucalipto merecem destaque devido ao impacto dessa prática no ambiente, principalmente sobre a atividade microbiana do solo e micro-organismos benéficos, como os fungos micorrízicos e os solubilizadores de fosfatos. Objetivou-se estudar o impacto da aplicação dos herbicidas sulfentrazona, isoxaflutol e oxyfluorfem sobre a colonização micorrízica, biomassa e atividade microbiana de dois solos florestais cultivados com eucalipto. O ensaio foi conduzido em casa de vegetação utilizando um solo argiloso e um solo francoarenoso distribuídos em vasos de 12 dm
3. O esquema fatorial utilizado foi 4 x 3, sendo três herbicidas (sulfentrazona, isoxaflutol e oxyfluorfem) mais a testemunha e três avaliações (5, 20 e 70 dias após a aplicação -DAA), no delineamento inteiramente casualizado com quatro repetições. Após a aplicação dos her...