Micro-aeration: an attractive strategy to facilitate anaerobic digestion

Fu, Shan-Fei; Lian, Shujuan; Angelidaki, Irini; Guo, Rongbo

doi:10.1016/j.tibtech.2022.09.008

Cited by 41 publications

(6 citation statements)

References 79 publications

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“…63 Selenocysteine could also be beneficial for microaeration strategies to enhance hydrolysis and methane production. 41,42 In this enrichment, the increased relative abundance of Methanobacterium and Methanobrevibacter corresponded to an escalating selenocysteine ratio, indicating that selenocysteine incorporation confers a competitive advantage (Figure 4). Interestingly, the oxygen-sensitive class I methanogens (Methanobacterium and Methanobrevibacter) dominated the H-ORP environments, while the L-ORP environment had increased abundance of the more oxygen-tolerant class II methanogens (Methanosarcina and Methanoculleus).…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…Selenocysteine incorporation could be beneficial in anaerobic digestion processes, in which operational conditions often lead to inadvertent exposure to oxygen, which impacts efficiency . Selenocysteine could also be beneficial for microaeration strategies to enhance hydrolysis and methane production. , …”

Section: Resultsmentioning

confidence: 99%

“…Deciphering the response of methanogens to varying redox environments and the role of selenocysteine will aid in advancing our understanding of biogeochemical cycles and enhance the design of more robust and efficient digestion and biogas production processes. , Therefore, this study presents a proteomic analysis of methanogenic cultures cultivated in the presence of the reducing agent 9,10-anthraquinone-2,7-disulfonate disodium (AQDS) and without AQDS. While the oxidation–reduction potential (ORP) was monitored with probes, proteomic analysis was utilized to examine the oxidative stress response and discern selenium-associated changes in the proteome.…”

Section: Introductionmentioning

confidence: 99%

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The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Kleikamp,

Palacios,

Kofoed

et al. 2024

Environ. Sci. Technol.

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Methanogenesis is a critical process in the carbon cycle that is applied industrially in anaerobic digestion and biogas production. While naturally occurring in diverse environments, methanogenesis requires anaerobic and reduced conditions, although varying degrees of oxygen tolerance have been described. Microaeration is suggested as the next step to increase methane production and improve hydrolysis in digestion processes; therefore, a deeper understanding of the methanogenic response to oxygen stress is needed. To explore the drivers of oxygen tolerance in methanogenesis, two parallel enrichments were performed under the addition of H2/CO2 in an environment without reducing agents and in a redox-buffered environment by adding redox mediator 9,10-anthraquinone-2,7-disulfonate disodium. The cellular response to oxidative conditions is mapped using proteomic analysis. The resulting community showed remarkable tolerance to high-redox environments and was unperturbed in its methane production. Next to the expression of pathways to mitigate reactive oxygen species, the higher redox potential environment showed an increased presence of selenocysteine and selenium-associated pathways. By including sulfur-to-selenium mass shifts in a proteomic database search, we provide the first evidence of the dynamic and large-scale incorporation of selenocysteine as a response to oxidative stress in hydrogenotrophic methanogenesis and the presence of a dynamic selenoproteome.

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Section: ■ Results and Discussionmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Kleikamp,

Palacios,

Kofoed

et al. 2024

Environ. Sci. Technol.

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“…Tahap terakhir adalah metanogenesis. Asam asetat dan hidrogen dari proses asetogenesis akan didegradasi menjadi gas metana dan karbon dioksida yang merupakan produk akhir dari rangkaian panjang proses peruraian anaerobic (Fu et al, 2022).…”

Section: Gambar 2 Skema Proses Fermentasi Anaerobikunclassified

Studi Potensi Asam Lemak Volatil Dari Tandan Kosong Kelapa Sawit Dengan Fermentasi Anaerob

Sanjaya¹

2023

novan

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Minyak sawit merupakan salah satu komoditas unggulan, dan Indonesia merupakan salah satu produsen kelapa sawit terbesar di dunia. Limbah padat yang dihasilkan dari aktivitas industri kelapa sawit adalah Tandan Kosong Kelapa Sawit (TKKS). Kandungan selulosa di dalam TKKS dapat dimanfaatkan sebagai substrat peruraian anaerob untuk menghasilkan volatile fatty acids (VFAs). Pada penelitian ini digunakan pengolahan awal secara kimia (NaOH 8% w/V) untuk delignifikasi TKKS sebelum diproses dalam fermentasi anaerob. Digested cow manure (DCM) digunakan sebagai inokulum. Pengoptimalan proses untuk menghasilkan VFAs merupakan tantangan karena VFAs merupakan produk intermediet di jalur metabolik peruraian anaerob menuju pembentukan metana. Penelitian ini mengkaji potensi VFAs dari TKKS dengan menvariasikan suhu terhadap proses fermentasi anaerobik TKKS untuk memproduksi VFAs di reaktor batch. Variasi suhu yang dikaji adalah kondisi mesofilik (29 ℃) dan termofilik (55 ℃). Prediksi potensi VFA dapat dihitung dengan rumus molekul empiris dengan pendekatan stoikiometri reaksi. Hasil perhitungan, potensi VFA dari TKKS adalah sebesar 44,5x10-2 mg.mgTS-1. Eksperimen menujukkan kondisi termofilik menghasilkan VFAs 60% lebih tinggi dibandingkan mesofilik, terlihat dari percent completion RT mencapai 59% dan lebih mendekati potensi teoritis.

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“…In recent years, micro-aeration has been pointed out as an attractive strategy to improve AD processes (Botheju and Bakke 2011 ; Fu et al 2023 ; Nguyen and Khanal 2018 ). Beneficial effects of injecting limited amounts of oxygen have been reported, namely scavenging H 2 S, enhancing the hydrolysis step, and avoiding volatile fatty acids (VFA) accumulation, thereby improving the overall stability of AD systems (Nguyen et al 2019b ; Nguyen and Khanal 2018 ; Tsapekos et al 2017 ; Xu et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of micro-aeration on syntrophic and methanogenic activity in anaerobic sludge

Morais,

Magalhães,

Martins

et al. 2024

Appl Microbiol Biotechnol

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Micro-aeration was shown to improve anaerobic digestion (AD) processes, although oxygen is known to inhibit obligate anaerobes, such as syntrophic communities of bacteria and methanogens. The effect of micro-aeration on the activity and microbial interaction in syntrophic communities, as well as on the potential establishment of synergetic relationships with facultative anaerobic bacteria (FAB) or aerobic bacteria (AB), was investigated. Anaerobic sludge was incubated with ethanol and increasing oxygen concentrations (0–5% in the headspace). Assays with acetate or H2/CO2 (direct substrates for methanogens) were also performed. When compared with the controls (0% O2), oxygen significantly decreased substrate consumption and initial methane production rate (MPR) from acetate or H2/CO2. At 0.5% O2, MPR from these substrates was inhibited 30–40%, and close to 100% at 5% O2. With ethanol, significant inhibition (>36%) was only observed for oxygen concentrations higher than 2.5%. Oxygen was consumed in the assays, pointing to the stimulation of AB/FAB by ethanol, which helped to protect the syntrophic consortia under micro-aerobic conditions. This highlights the importance of AB/FAB in maintaining functional and resilient syntrophic communities, which is relevant for real AD systems (in which vestigial O2 amounts are frequently present), as well as for AD systems using micro-aeration as a process strategy. Key points •Micro-aeration impacts syntrophic communities of bacteria and methanogens. •Oxygen stimulates AB/FAB, maintaining functional and resilient consortia. •Micro-aeration studies are critical for systems using micro-aeration as a process strategy.

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Micro-aeration: an attractive strategy to facilitate anaerobic digestion

Cited by 41 publications

References 79 publications

The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Studi Potensi Asam Lemak Volatil Dari Tandan Kosong Kelapa Sawit Dengan Fermentasi Anaerob

Effect of micro-aeration on syntrophic and methanogenic activity in anaerobic sludge

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