Micro‐ and Nanopatterning of Active Biomolecules and Cells

Aydin, Daniel; Hirschfeld-Warneken, Vera C.; Louban, Ilia; Spatz, Joachim P.

doi:10.1002/9781118181249.ch8

Cited by 3 publications

(1 citation statement)

References 102 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 kDa and 20 kDa PEG (Sigma-Aldrich, MO, USA) were diacrylated by following the procedure used by Aydin et al 60 but omitting the filtration step over a plug of alumina. The synthesized PEG diacrylate was dissolved in DI water to achieve a 15 wt% solution.…”

Section: Peg Diacrylate Synthesis and Gelationmentioning

confidence: 99%

The structural fate of lipid nanoparticles in the extracellular matrix

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Peg Diacrylate Synthesis and Gelationmentioning

confidence: 99%

The structural fate of lipid nanoparticles in the extracellular matrix

et al. 2020

View full text Add to dashboard Cite

show abstract

Enzyme Prodrug Therapy Engineered into Biomaterials

Mendes

Zelikin

2014

Adv Funct Materials

View full text Add to dashboard Cite

In this work, enzyme-prodrug therapy (EPT) is engineered into hydrogel biomaterials to achieve localized synthesis of the drugs and their delivery to the adhering cells. The use of EPT in the context of drug delivery mediated by biomaterials signifi cantly empowers the latter in that the same hydrogel is used to successfully synthesize several drugs with dissimilar structures and therapeutic effects. The concentration of the synthesized drugs is conveniently controlled by the concentration of the administered prodrugs. Using prodrugs for two therapeutic agents allows their synthesis and delivery with independent control over the concentration and the time of administration of each of the drugs. Using these tools, sequential delivery of drugs for anti-infl ammatory and anti-proliferative activity is accomplished whereby the synthesis of drugs is mediated by the same enzyme-functionalized hydrogel. The use of EPT to perform combination therapy mediated by an implantable biomaterial is also reported. Taken together, these results contribute signifi cantly to the development of fl exible and highly powerful tools of substrate-mediated drug delivery with applications in the design of therapeutic implants and tissue engineering.

show abstract

Microstructured, Functional PVA Hydrogels through Bioconjugation with Oligopeptides under Physiological Conditions

Chong¹,

Smith²,

Zelikin³

2012

Small

View full text Add to dashboard Cite

In this work, bioconjugation techniques are developed to achieve peptide functionalization of poly(vinyl alcohol), PVA, as both a polymer in solution and within microstructured physical hydrogels, in both cases under physiological conditions. PVA is unique in that it is one of very few polymers with excellent biocompatibility and safety and has FDA approval for clinical uses in humans. However, decades of development have documented only scant opportunities in bioconjugation with PVA. As such, materials derived thereof fail to answer the call for functional biomaterials for advanced cell culture and tissue engineering applications. To address these limitations, PVA is synthesized with terminal thiol groups and conjugated with thiolated peptides using PVA in solution. Further, microstructured, surface-adhered PVA physical hydrogels are assembled, the available conjugation sites within the hydrogels are quantified, and quantitative kinetic data are collected on peptide conjugation to the hydrogels. The success of bioconjugation in the gel phase is quantified through the use of a cell-adhesive peptide and visualization of cell adhesion on PVA hydrogels as cell culture substrates. Taken together, the presented data establish a novel paradigm in bioconjugation and functionalization of PVA physical hydrogels. Coupled with an excellent safety profile of PVA, these results deliver a superior biomaterial for diverse biomedical applications.

show abstract

Micro‐ and Nanopatterning of Active Biomolecules and Cells

Cited by 3 publications

References 102 publications

The structural fate of lipid nanoparticles in the extracellular matrix

The structural fate of lipid nanoparticles in the extracellular matrix

Enzyme Prodrug Therapy Engineered into Biomaterials

Microstructured, Functional PVA Hydrogels through Bioconjugation with Oligopeptides under Physiological Conditions

Contact Info

Product

Resources

About