Micro and Nanotechnologies Enhanced Biomolecular Sensing

Wang, Tza‐Huei

doi:10.3390/bios3030283

Cited by 4 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Manipulating objects and particles at the submicrometer and nanometer ranges is of great importance in many biochemical and biomedical applications, such as the study of bacteria–host cell interaction, , nanoparticle–cell interaction, − sample enrichment for diagnostics, ,− bioassays, − and food and environmental monitoring . However, only a handful of methods can achieve some form of control over nanoparticles due to their small size.…”

mentioning

confidence: 99%

Enriching Nanoparticles via Acoustofluidics

et al. 2017

View full text Add to dashboard Cite

Focusing and enriching submicrometer and nanometer scale objects is of great importance for many applications in biology, chemistry, engineering, and medicine. Here, we present an acoustofluidic chip that can generate single vortex acoustic streaming inside a glass capillary through using low-power acoustic waves (only 5 V is required). The single vortex acoustic streaming that is generated, in conjunction with the acoustic radiation force, is able to enrich submicrometer- and nanometersized particles in a small volume. Numerical simulations were used to elucidate the mechanism of the single vortex formation and were verified experimentally, demonstrating the focusing of silica and polystyrene particles ranging in diameter from 80 to 500 nm. Moreover, the acoustofluidic chip was used to conduct an immunoassay in which nanoparticles that captured fluorescently labeled biomarkers were concentrated to enhance the emitted signal. With its advantages in simplicity, functionality, and power consumption, the acoustofluidic chip we present here is promising for many point-of-care applications.

show abstract

mentioning

confidence: 99%

Enriching Nanoparticles via Acoustofluidics

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This area is critical for the management of infectious diseases by providing the possibility of prompt therapeutic intervention. In this context, nucleic acid-based detection can bring forth higher sensitivity and shorter turnaround time (see, e.g., [ 239 ] and references therein). Furthermore, enhanced sensitivity and multiplexing capabilities can be achieved by the coupling of electrochemical biosensors with nanoparticles.…”

Section: Further Characteristics and Areas Of Applicationsmentioning

confidence: 99%

“…Nanocluster-based DNA/RNA probe designs are diverse and their scope is indeed impressive. Out of a myriad of applications of such biosensors they can be applied to the detection and monitoring of environmental pollutants, new chemicals, and drugs, including those producing genotoxicity with damages to the genetic information within a cell via mutations ([ 239 ] and references therein). As an example, Figure 7 presents the electrochemical aptasensor for multiplex detection of growth factors that regulate cell growth and division, such as the platelet-derived growth factor (PDGF-BB), as well as multifunctional serine proteases that play a key role in haemostasis, such as thrombin.…”

Section: Further Characteristics and Areas Of Applicationsmentioning

confidence: 99%

Coupled Multiphysics Modelling of Sensors for Chemical, Biomedical, and Environmental Applications with Focus on Smart Materials and Low-Dimensional Nanostructures

Singh

Melnik

2022

Chemosensors

View full text Add to dashboard Cite

Low-dimensional nanostructures have many advantages when used in sensors compared to the traditional bulk materials, in particular in their sensitivity and specificity. In such nanostructures, the motion of carriers can be confined from one, two, or all three spatial dimensions, leading to their unique properties. New advancements in nanosensors, based on low-dimensional nanostructures, permit their functioning at scales comparable with biological processes and natural systems, allowing their efficient functionalization with chemical and biological molecules. In this article, we provide details of such sensors, focusing on their several important classes, as well as the issues of their designs based on mathematical and computational models covering a range of scales. Such multiscale models require state-of-the-art techniques for their solutions, and we provide an overview of the associated numerical methodologies and approaches in this context. We emphasize the importance of accounting for coupling between different physical fields such as thermal, electromechanical, and magnetic, as well as of additional nonlinear and nonlocal effects which can be salient features of new applications and sensor designs. Our special attention is given to nanowires and nanotubes which are well suited for nanosensor designs and applications, being able to carry a double functionality, as transducers and the media to transmit the signal. One of the key properties of these nanostructures is an enhancement in sensitivity resulting from their high surface-to-volume ratio, which leads to their geometry-dependant properties. This dependency requires careful consideration at the modelling stage, and we provide further details on this issue. Another important class of sensors analyzed here is pertinent to sensor and actuator technologies based on smart materials. The modelling of such materials in their dynamics-enabled applications represents a significant challenge as we have to deal with strongly nonlinear coupled problems, accounting for dynamic interactions between different physical fields and microstructure evolution. Among other classes, important in novel sensor applications, we have given our special attention to heterostructures and nucleic acid based nanostructures. In terms of the application areas, we have focused on chemical and biomedical fields, as well as on green energy and environmentally-friendly technologies where the efficient designs and opportune deployments of sensors are both urgent and compelling.

show abstract

“…In particular, progress in synthesis chemistry has allowed the creation of monodisperse particles of controlled sizes, shapes and compositions, thereby providing a way to precisely manipulate material properties [4]. Specifically, nano-and micrometer-sized magnetic particles have proved to be valuable due to their unique properties, such as magnetization under the influence of an external field and their large surface-to volume ratio.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different strategies for magnetic particle functionalization with DNA aptamers

2016

View full text Add to dashboard Cite

The optimal bio-functionalization of magnetic particles is essential for developing magnetic particle-based bioassays. Whereas functionalization with antibodies is generally well established, immobilization of DNA probes, such as aptamers, is not yet fully explored. In this work, four different types of commercially available magnetic particles, coated with streptavidin, maleimide or carboxyl groups, were evaluated for their surface coverage with aptamer bioreceptors, efficiency in capturing target protein and non-specific protein adsorption on their surface. A recently developed aptamer against the peanut allergen, Ara h 1 protein, was used as a model system. Conjugation of biotinylated Ara h 1 aptamer to the streptavidin particles led to the highest surface coverage, whereas the coverage of maleimide particles was 25% lower. Carboxylated particles appeared to be inadequate for DNA functionalization. Streptavidin particles also showed the greatest target capturing efficiency, comparable to the one of particles functionalized with anti-Ara h 1 antibody. The performance of streptavidin particles was additionally tested in a sandwich assay with the aptamer as a capture receptor on the particle surface. While the limit of detection obtained was comparable to the same assay system with antibody as capture receptor, it was superior to previously reported values using the same aptamer in similar assay schemes with different detection platforms. These results point to the promising application of the Ara h 1 aptamer-functionalized particles in bioassay development.

show abstract

Micro and Nanotechnologies Enhanced Biomolecular Sensing

Cited by 4 publications

References 5 publications

Enriching Nanoparticles via Acoustofluidics

Enriching Nanoparticles via Acoustofluidics

Coupled Multiphysics Modelling of Sensors for Chemical, Biomedical, and Environmental Applications with Focus on Smart Materials and Low-Dimensional Nanostructures

Evaluation of different strategies for magnetic particle functionalization with DNA aptamers

Contact Info

Product

Resources

About