Micro and nanotechnologies in heart valve tissue engineering

Hasan, Anwarul; Saliba, John; Modarres, Hassan Pezeshgi; Bakhaty, Ahmed A.; Nasajpour, Amir; Mofrad, Mohammad R. K.; Sanati‐Nezhad, Amir

doi:10.1016/j.biomaterials.2016.07.001

Cited by 47 publications

(21 citation statements)

References 218 publications

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“…Therefore the structural, mechanical, and compositional scaffold design parameters discussed above were measured and evaluated for each JetValve prepared for implantation as a factor of safety for the animal models used. The automated method of JetValve assembly and simplicity of manufacturing customization enabled straightforward implementation of multi-parameter process capability metrics [55, 56]. Our group has developed similar multi-parameter quality assessment indices for stem cell manufacturing [27, 57] and suggest that the same can and should be done for tissue engineered products [19].…”

Section: Discussionmentioning

confidence: 99%

“…Inclusion of these factors as dopants is possible using the JetValve manufacturing method without significant modification to the technique or time to production. Growth factors associated with development such as transforming growth factor-β1 (TGF-β1), bone morphogenic proteins (BMPs), and/or platelet-derived growth factors (PDGFs) may be incorporated into scaffolds to elicit endothelial-to-mesenchymal transformation (EMT), for example, in order to populate and remodel the scaffold [55, 62, 63]. Additionally, recently reported hybrid-manufacturing techniques for the production of more complex, tri-layered scaffolds have been developed in an effort to better mimic the anisotropic, stratified structure of the valvular ECM for optimal hemodynamic performance and tissue regeneration [52, 64–66].…”

Section: Discussionmentioning

confidence: 99%

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JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement

et al. 2017

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Tissue engineered scaffolds have emerged as a promising solution for heart valve replacement because of their potential for regeneration. However, traditional heart valve tissue engineering has relied on resource-intensive, cell-based manufacturing, which increases cost and hinders clinical translation. To overcome these limitations, in situ tissue engineering approaches aim to develop scaffold materials and manufacturing processes that elicit endogenous tissue remodeling and repair. Yet despite recent advances in synthetic materials manufacturing, there remains a lack of cell-free, automated approaches for rapidly producing biomimetic heart valve scaffolds. Here, we designed a jet spinning process for the rapid and automated fabrication of fibrous heart valve scaffolds. The composition, multiscale architecture, and mechanical properties of the scaffolds were tailored to mimic that of the native leaflet fibrosa and assembled into three dimensional, semilunar valve structures. We demonstrated controlled modulation of these scaffold parameters and show initial biocompatibility and functionality in vitro. Valves were minimally-invasively deployed via transapical access to the pulmonary valve position in an ovine model and shown to be functional for 15 hours.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement

et al. 2017

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“…One important benefit of microscale tissues synthesized within microfluidics is the ability to produce stable and long‐lasting chemical and biological gradients to cells encapsulated within microgels, essential to achieve physiologic relevance of in vitro tissue models . Cosson and Lutolf developed a hydrogel microfluidics for spatiotemporal delivery of molecules to mESCs in the form of biomolecular gradients ( Figure a).…”

Section: Microfluidics For the Controlled Differentiation Of Stem Cellsmentioning

confidence: 99%

Controlling Differentiation of Stem Cells for Developing Personalized Organ‐on‐Chip Platforms

Geraili

Jafari

Hassani

et al. 2017

Adv Healthcare Materials

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Organ‐on‐chip (OOC) platforms have attracted attentions of pharmaceutical companies as powerful tools for screening of existing drugs and development of new drug candidates. OOCs have primarily used human cell lines or primary cells to develop biomimetic tissue models. However, the ability of human stem cells in unlimited self‐renewal and differentiation into multiple lineages has made them attractive for OOCs. The microfluidic technology has enabled precise control of stem cell differentiation using soluble factors, biophysical cues, and electromagnetic signals. This study discusses different tissue‐ and organ‐on‐chip platforms (i.e., skin, brain, blood–brain barrier, bone marrow, heart, liver, lung, tumor, and vascular), with an emphasis on the critical role of stem cells in the synthesis of complex tissues. This study further recaps the design, fabrication, high‐throughput performance, and improved functionality of stem‐cell‐based OOCs, technical challenges, obstacles against implementing their potential applications, and future perspectives related to different experimental platforms.

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“…The employment of this technology has promoted the emergence of micro-scale technologies and microfluidic systems, which are useful tools to overcome the challenges of creating artificial microvascular structures [6][7][8]. Consequently, these tools make bioprinting even more efficient in terms of versatility, detailing, and capacity to obtain structures with high spatial resolution [9]. Furthermore, hydrogels have been widely studied due to their unique properties such as biocompatibility and bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Printing 3D Hydrogel Structures Employing Low-Cost Stereolithography Technology

Magalhães

Santos

Elias³

et al. 2020

JFB

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Stereolithography technology associated with the employment of photocrosslinkable, biocompatible, and bioactive hydrogels have been widely used. This method enables 3D microfabrication from images created by computer programs and allows researchers to design various complex models for tissue engineering applications. This study presents a simple and fast home-made stereolithography system developed to print layer-by-layer structures. Polyethylene glycol diacrylate (PEGDA) and gelatin methacryloyl (GelMA) hydrogels were employed as the photocrosslinkable polymers in various concentrations. Three-dimensional (3D) constructions were obtained by using the stereolithography technique assembled from a commercial projector, which emphasizes the low cost and efficiency of the technique. Lithium phenyl-2,4,6-trimethylbenzoyl phosphonate (LAP) was used as a photoinitiator, and a 404 nm laser source was used to promote the crosslinking. Three-dimensional and vascularized structures with more than 5 layers and resolutions between 42 and 83 µm were printed. The 3D printed complex structures highlight the potential of this low-cost stereolithography technique as a great tool in tissue engineering studies, as an alternative to bioprint miniaturized models, simulate vital and pathological functions, and even for analyzing the actions of drugs in the human body.

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Micro and nanotechnologies in heart valve tissue engineering

Cited by 47 publications

References 218 publications

JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement

JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement

Controlling Differentiation of Stem Cells for Developing Personalized Organ‐on‐Chip Platforms

Printing 3D Hydrogel Structures Employing Low-Cost Stereolithography Technology

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