Micro-array chip for high throughput protein crystallography

The applicability of the heteroepitaxic nucleant method using molecular sieves (MS) to sparse matrix screening of protein crystallization was examined using xylanase as a test protein. The sparse matrix screening with MS provided three crystal forms I-III under 80% of conditions, which confirmed a much higher yield of crystal formation when compared to the conventional screening without MS. In the form I crystals, an MS-dependent improvement in X-ray diffraction quality was observed. Furthermore, form III of xylanase crystals was obtained only in the presence of MS 5A or 13X, demonstrating an MS-dependent packing-space expansion of protein crystals.

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Packing Space Expansion of Protein Crystallization Screening with Synthetic Zeolite as a Heteroepitaxic Nucleant

Sugahara

Kageyama-Morikawa

Kunishima

2010

Crystal Growth & Design

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SLS Crystallization Platform at Beamline X06DA—A Fully Automated Pipeline Enabling in Situ X-ray Diffraction Screening

Bingel-Erlenmeyer

Oliéric

Grimshaw

et al. 2011

Crystal Growth & Design

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Crystal Growth & DesignCOMMUNICATION together with the beamline automation at X06DA, this is a clear step toward a much more streamlined and successful work-flow in macromolecular crystallography, eliminating significant bottlenecks in the process.' ASSOCIATED CONTENT b S Supporting Information. Movie showing the workflow and isXds at the SLS crystallization platform at beamline X06DA. This material is available free of charge via the Internet at http:// pubs.acs.org.

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Micro-array chip for high throughput protein crystallography

Cited by 2 publications

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Packing Space Expansion of Protein Crystallization Screening with Synthetic Zeolite as a Heteroepitaxic Nucleant

Packing Space Expansion of Protein Crystallization Screening with Synthetic Zeolite as a Heteroepitaxic Nucleant

SLS Crystallization Platform at Beamline X06DA—A Fully Automated Pipeline Enabling in Situ X-ray Diffraction Screening

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