In this study, we report on the inactivation technique to apply the oral surgery by micro low-temperature and atmospheric-pressure plasma irradiation utilizing a capillary nozzle. Emission spectrum of the micro-plasma showed OH and O radical, which were affected inactivation of coliform. Vibration temperature of the micro-plasma with 7998 K was calculated from peak-intensity at emission due to N22nd positive system. Inactivation region was larger than plasma irradiation spot, and it was increasing with plasma irradiation time.