Micro-computed tomographic and SEM study of porous bioceramics using an adaptive method based on the mathematical morphological operations

Abstract

Numerous clinical studies have demonstrated the influence of the size, number and shape of pores into calcium phosphate ceramics on the process of bone regeneration.The main objective of this study is to determine the microstructure, the morphological characteristics and classes of pores of the prepared hydroxyapatite bioceramic using an adaptive method based on the mathematical morphological operations. The study was carried out using X-ray microtomography and Scanning Electron Microscopy images. The conventional method of openings alone presents limitation of calculation and not sufficient to achieve our objective. The proposed method allowed us to extract local characteristics and calculate precisely the morphological parameters while preserving the original volume of pores. The number and classes of pores with their size, surface of contact of the component and the number of connected pores to each pore were calculated. The method is subjectively and quantitatively evaluated using different computed phantoms and its efficiency is clearly demonstrated through the different reports and measurements generated. The proposed method can have interesting applications in the characterization of porous materials used in the medical field or in other sectors.

Regeneration of Rabbit Calvarial Defects with Combination of Stem Cells and Enamel Matrix Derivative: A Microcomputed Tomography and Histological Evaluation Comparing Two- and Three-Dimensional Cell Constructs

“…Micro-CT can provide detailed three-dimensional evaluation of calvarial defects, providing crucial quantitative data on bone density, structure, and morphology, which is integral for monitoring treatment efficacy and understanding defect dynamics [ 45 ]. Its high-resolution, non-destructive imaging capabilities, and minimal sample preparation requirements render micro-CT an invaluable tool in both research and clinical diagnostics [ 46 , 47 , 48 ]. Micro-CT provided extremely high-resolution images of bone structures, allowing for detailed visualization of calvarial defects [ 46 ].…”

“…Micro-CT provided extremely high-resolution images of bone structures, allowing for detailed visualization of calvarial defects [ 46 ]. Micro-CT is characterized by its non-invasive nature, which enables the analysis of samples without necessitating any alteration or damage to the specimen being examined [ 47 ]. Compared to other imaging modalities that may necessitate extensive sample preparation, micro-CT requires reduced preparatory steps, rendering a more expedient and practical option for many analytical applications [ 46 ].…”

Osseointegration properties of domestic bioactive calcium phosphate ceramics doped with silicon

“…The results of long-term experiments with β-TCP indicate that it can only possess osteoconductive properties. 16,24 Numerous studies of bone tissue replacement with calciumphosphate ceramics have shown the important role of porosity in this process. 19,24 Volumetric porosity and pore size affect the ability of CPС to promote vascularization, cell proliferation and osteoconduction in vivo.…”

Automatic computation of bone defective volume from tomographic images

“…The early osseointegration of bone implants is linked to their long-term therapeutic success [ 4 ]. The stability of the implant is mostly determined by achieving the best possible contact between the bone and the implanted material [ 5 , 6 ]. Predicting the shape and volume of the cavity to be filled can aid in successful implantation [ 7 ].…”