Micro-computed tomography and microdissection of the temporal bone of tree shrews

Xie, Lihong; Gyanwali, Bibek; Zhu, Meichan; Kaiquan, Zhu; Sun, Kai; Tang, Anzhou

doi:10.1016/j.aanat.2015.08.005

Cited by 8 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous studies, we found that the morphology of ear such as the number of cochlear circles and ear ossicles of tree shrews is closer to that of humans than guinea pigs and rats. In this paper, we further examined hearing function by using ABRs on the basis of our original study of the temporal bone anatomy in tree shrews (Lihong et al., 2016; Xu et al., 2012). The goal of the current study was to evaluate the patterns of ABR waves in healthy adult tree shrews by click and tone-pips stimuli and to examine the auditory sensitivity and hearing range.…”

Section: Introductionmentioning

confidence: 99%

The characterization of auditory brainstem response (ABR) waveforms: A study in tree shrews (Tupaia belangeri)

et al. 2018

View full text Add to dashboard Cite

To characterize the patterns of ABR waves in tree shrews, we must understand the hearing sensitivity and auditory function of healthy adult tree shrews. Fifteen tree shrews (30 ears) were stimulated with clicks and tone-pips at 11 different frequencies from 1 to 60 kHz. The ABR waves were recorded and analyzed. The ABR consisted of five to seven positive waves in the first 10 ms after a click stimulus, and the average hearing threshold of component III was 27.86 ± 3.78 dB SPL. Wave III was the largest and most clear. The ABR threshold was related to the tone-pip sitmulus by a “U” shaped curve. The sensitive frequency was approximately 8 kHz in tree shrews. The latencies systematically decreased with increasing stimulus frequencies. The ABR amplitudes of wave III increased as the sound pressure level increased. All of these results provide an empirical basis for future studies of hearing diseases in tree shrews.

show abstract

Section: Introductionmentioning

confidence: 99%

The characterization of auditory brainstem response (ABR) waveforms: A study in tree shrews (Tupaia belangeri)

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Given the aforementioned findings, HRMRIs and micro computed tomography would be helpful to further quantify the non-bony components of the CA. 8,28 Despite the uncommon utilization in otosclerosis, supplementary HRMRI with fluid length and width calculations could be used to further understand the CA patency prior to making further conclusions.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Computed Tomography of the Inner Ear: Effect of Otosclerosis on Cochlear Aqueduct Dimensions

Wichova

Alvi

Boatright

et al. 2019

Ann Otol Rhinol Laryngol

View full text Add to dashboard Cite

Objectives: The cochlear aqueduct is a bony duct connecting the scala tympani with the subarachnoid space. Given the pathophysiology of otosclerosis, including bone resorption and new bone deposition, we hypothesize that the cochlear aqueduct in otosclerotic ears is narrowed. Methods: A retrospective review of patients with otosclerosis who have undergone high-resolution computed tomography (HRCT) of the temporal bone was completed. The control cohort included 20 patients with the diagnosis of noise-induced hearing loss, without the diagnosis of otosclerosis. Uniform measurements of cochlear aqueduct dimensions were performed using the axial plane. Results: The otosclerosis cohort included 25 males and 52 females with mean age of 52.2 ± 17.6 years. The control group included 10 males and 10 females with mean age of 64.0 ± 18.5 years. The mean cochlear aqueduct length, width mid canal, aperture base, aperture widest diameter, and funnel diameter in millimeters were 12.19 ± 1.66, 0.68 ± 0.28, 4.21 ± 1.67, 3.23 ± 1.47, and 2.70 ± 1.05 in the ears with otosclerotic foci and 11.57 ± 1.66, 0.69 ± 0.29, 2.56 ± 1.59, 2.77 ± 1.67, and 2.58 ± 1.03 in control group, respectively. Statistical difference was seen in length of cochlear aqueduct, aperture base, and aperture widest diameters ( P = .017, <.001, .007). Conclusions: The length of the cochlear aqueduct and the funnel width are statistically longer in the otosclerotic population compared to control. The width of the cochlear aqueduct is not statistically different.

show abstract

“…The tree shrew is a novel model organism for studying human brain disease (31). Micro-computed tomography and microdissection show that the cochlear structure and cochlear rotations of the tree shrew are highly similar to those of humans, and thus, tree shrews can be used for research on ear diseases (32). The lungs of the tree shrew have three left lobes and four right lobes, whereas the lungs of humans have only two left lobes and three right lobes.…”

Section: The Tree Shrew Anatomymentioning

confidence: 99%

The Tree Shrew as a Model for Cancer Research

Peng

Zhong

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Animal disease models are necessary in medical research, and an appropriate animal model is of great importance for studies about the prevention or treatment of cancer. The most important thing in the selection of animal models is to consider the similarity between animals and humans. The tree shrew (Tupaia belangeri) is a squirrel-like mammal which placed in the order Scandentia. Whole-genome sequencing has revealed that tree shrews are extremely similar to primate and humans than to rodents, with many highly conserved genes, which makes the data from studies that use tree shrews as models more convincing and the research outcomes more easily translatable. In tumor research, tree shrews are often used as animal models for hepatic and mammary cancers. As research has progressed, other types of tree shrew tumor models have been developed and exhibit clinical manifestations similar to those of humans. Combining the advantages of both rodents and primates, the tree shrew is expected to be the most powerful animal model for studying tumors.

show abstract

Micro-computed tomography and microdissection of the temporal bone of tree shrews

Cited by 8 publications

References 16 publications

The characterization of auditory brainstem response (ABR) waveforms: A study in tree shrews (Tupaia belangeri)

The characterization of auditory brainstem response (ABR) waveforms: A study in tree shrews (Tupaia belangeri)

High-Resolution Computed Tomography of the Inner Ear: Effect of Otosclerosis on Cochlear Aqueduct Dimensions

The Tree Shrew as a Model for Cancer Research

Contact Info

Product

Resources

About