Micro‐CT dilatometry measures of molecular collagen hydration using bovine extensor tendon

Fullerton, Gary D.; Amurao, Maxwell; Rahal, Andres; Cameron, Ivan L.

doi:10.1118/1.3514123

Cited by 11 publications

(10 citation statements)

References 48 publications

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“…Validation studies by Martiniova et al demonstrated that the accuracy of T2-weighted MRI volume measurements was within about 8% [22]. In the present study, unlike the prior studies, in-air micro-CT image volume of excised tumors was calculated as reference tumor volume using the methodology previously shown by Fullerton et al [14]. Using the reference tumor volume, then image-based volumes were quantified and compared among three commonly used imaging modalities (US, micro-CT and MRI) in rodent tumor models.…”

Section: Introductionmentioning

confidence: 78%

“…To our knowledge, the accuracy of in-air micro-CT image volume had not previously been validated for tumor studies and it has not been used before as a reference/true tumor volume. Fullerton et al in the same research group initially proved that in-air micro-CT image volume is fairly accurate (~0.6%) [14] and we adopted the method. For this study, we assumed that no dehydration of excised tumors occurred during the time (less than 10 min) between tumor excision and completion of the micro-CT scans.…”

Section: In-air Micro-ct Image Volume As Alternative Gold Standardmentioning

confidence: 99%

“…Recently, Fullerton et al confirmed the high accuracy of in-air micro-computed tomography (micro-CT) image (i.e. micro-CT image of a sample surrounded by air) volume by a voxel counting method using National Institute of Standards and Technology (NIST) traceable cylindrical phantoms [14]. Micro-CT resolution is typically at the micron level [15].…”

Section: Introductionmentioning

confidence: 99%

“…When an excised tumor is placed in a high contrast radiographic background such as air, the tumor size on the order of ten microns can be readily measured. This high contrast between tumor and air allows for accurate voxel counting for calculation of tumor volume in the micro-CT images [14]. In addition, in comparison to magnetic resonance imaging (MRI), micro-CT is more cost-effective [16] and allows for much faster scan time, which can prevent dehydration of excised tumors.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume

Lee¹,

Fullerton²,

Goins³

2015

OJMI

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Purpose: Changes in tumor volume are used for therapy response monitoring in preclinical studies. Unlike prior studies, this article introduces in-air micro-computed tomography (micro-CT) image volume as reference tumor volume in rodent tumor models. Tumor volumes determined using imaging modalities such as magnetic resonance imaging (MRI), micro-CT and ultrasound (US), and with an external caliper are compared with the reference tumor volume. Materials and Methods: In vivo MR, US and micro-CT imaging was performed 4, 6, 9, 11 and 13 days after tumor cell inoculation into nude rats. On the day of the imaging study, in vivo caliper measurements were also made. After in vivo imaging, tumors were excised followed by in-air micro-CT imaging and ex vivo caliper measurements of excised tumors. The in-air micro-CT image volume of excised tumors was determined as reference tumor volume. Then tumor volumes were calculated using formula V = (π/6) × a × b × c, where a, b and c are maximum diameters in three perpendicular dimensions determined by the three image modalities and caliper, and compared with reference tumor volume by linear regression analysis as well as Bland-Altman plots. Results: The correlation coefficients (R 2) of the regression lines for in vivo tumor volumes measured by the three imaging modalities were 0.9939, 0.9669 and 0.9806 for MRI, US and micro-CT respectively. For caliper measurements, the coefficients were 0.9274 and 0.9819 for caliper in vivo and caliper ex vivo respectively. In Bland-Altman plots, the average of tumor volume difference from reference tumor volume (bias) was significant for caliper and micro-CT, but not for MRI and US. Conclusion: Using the in-air micro-CT image volume as reference tumor volume, tumor volume measured by MRI was the most accurate among the three imaging modalities. In vivo caliper volume measurements showed unreliability while ex vivo caliper measurements reduced errors.

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Section: Introductionmentioning

confidence: 78%

Section: In-air Micro-ct Image Volume As Alternative Gold Standardmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume

Lee¹,

Fullerton²,

Goins³

2015

OJMI

Self Cite

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“…The viscous thick albumen hen egg white is a hydrogel fraction and can be separated from the thin egg white sol fraction by sieve filtration (Cameron and Fullerton 2010). The shaking of thick albumen gel caused the transition from a gel state to a sol state as demonstrated by flow through the sieve filter.…”

Section: Physical Measured Properties Of Bulk and Non-bulk Water Fracmentioning

confidence: 99%

Untitled

2014

WATER

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This report reviews evidence on the physical properties and size, in g water per g dry mass (g/g) of multiple non-bulk water of hydration fractions on proteins and in cells. A molecular stoichiometric hydration model (SHM) is presented that explains the four observed and measured monolayer water of hydration fractions on tendon collagen (Fullerton et al. 2006 a , Fullerton andCameron 2007). This SHM has been shown applicable to globular proteins and to cells (Cameron et al. 2011). The extent of nonbulk water has been found to increase during protein unfolding and decrease during protein aggregation (Fullerton et al. 2006). This review also presents evidence that multilayers of water, with non-bulk water properties, extend out from the surface of proteins and biomacromolecules in cells. These facts have been largely overlooked or ignored by most protein chemists and cell biologists. In the conclusion it is recalled that a major fraction of cell water has physical and physiological properties that differ from those of bulk water. Thus studies that do not take the physical properties and size of non-bulk water fractions into account must be judged incomplete. IntroductionThe presence and extent of multiple water of hydration fractions on proteins and in cells has been a subject of debate (Ball 2008, Pollack and Clegg 2008, Fullerton and Cameron 2007, Cameron and Fullerton 2008, Cameron, Lanctot and Fullerton 2011. The physical characteristics and size of multiple water of hydration fractions has been measured on tendon/collagen by water proton NMR spin-lattice relaxation at different levels of collagen hydration (Fullerton et al. 2006). Three distinct hydration compartments were identified and their size quantified. The sizes were defined by Abbreviations: g/g grams water per gram dry mass, SHM stoichiometric hydration model, SASA solvent accessible surface area, QENS quasi-elastic neutron scattering spectroscopy, NMR nuclear magnetic resonance, CD circular dichroism spectroscopy, VW vicinal water, EZ exclusion zone water, PML polarized multilayered water, OUR osmotically unresponsize water, TEM transmission electron microscopy.

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Physical Chemistry of Collagen: The Molecular Basis of Magic Angle Contrast

Fullerton

2012

Encyclopedia of Magnetic Resonance

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Micro‐CT dilatometry measures of molecular collagen hydration using bovine extensor tendon

Cited by 11 publications

References 48 publications

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume

Untitled

Physical Chemistry of Collagen: The Molecular Basis of Magic Angle Contrast

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Micro‐CT dilatometry measures of molecular collagen hydration using bovine extensor tendon

Cited by 11 publications

References 48 publications

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using &lt;i&gt;In-Air&lt;/i&gt; Micro-CT Image Volume as Reference Tumor Volume

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using &lt;i&gt;In-Air&lt;/i&gt; Micro-CT Image Volume as Reference Tumor Volume

Untitled

Physical Chemistry of Collagen: The Molecular Basis of Magic Angle Contrast

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Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume

Comparison of Multimodality Image-Based Volumes in Preclinical Tumor Models Using <i>In-Air</i> Micro-CT Image Volume as Reference Tumor Volume