Micro-CT evaluation of asymmetrical ovine intervertebral disc height loss from surgical approach

Ludtka, Christopher; Schwan, Stefan; Friedmann, Andrea; Brehm, W; Wiesner, Ingo; Goehre, Felix

doi:10.1007/s00586-017-5024-8

Cited by 8 publications

(11 citation statements)

References 24 publications

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“…Figure 4 shows the change in IVD height at nine different points for both groups according to a previously described method. 18 In Group 1 (Fig. 4A), a significant reduction was only seen in two of the nine points ("anterior middle" [AM], p = 0.039; "center right" (CR), p = 0.038).…”

Section: Resultsmentioning

confidence: 96%

“…Figure shows the change in IVD height at nine different points for both groups according to a previously described method . In Group 1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The injury to the AF was made on the right side, which can lead to an uneven contralateral disc height between that and the contralateral side. The significance of such tilting can be assessed using a previously described measurement method . A comparison of the IVD heights on the right (injury) side and left (non‐injury) side can illustrate the anatomical effects of unilateral partial nucleotomy and allows assessment of whether the IVD injury leads to a side‐dependent loss of IVD height.…”

Section: Discussionmentioning

confidence: 99%

“…The significance of such tilting can be assessed using a previously described measurement method. 18 A comparison of the IVD heights on the right (injury) side and left (non-injury) side can illustrate the anatomical effects of unilateral partial nucleotomy and allows assessment of whether the IVD injury leads to a sidedependent loss of IVD height. For Group 1, there was no sign of an uneven reduction of the IVD level between the two sides.…”

Section: Discussionmentioning

confidence: 99%

“…After fixation in 2.5% (v/v) glutaraldehyde phosphate buffered saline for a minimum of 7 days, FSUs were refrozen and trisected in the anterior–posterior plane while frozen to form three sagittal sections left/middle/right according to the definition in Ludtka et al The samples were subsequently dehydrated using a rising series of ethanol (48 h soak per 10 m% step). To prepare hard and soft tissue components of the FSU with minimal artifacts, the samples were embedded in Poly(methyl methacrylate) (PMMA) using a Technovit9100 (Heraeus, Kulzer, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

Schwan

Ludtka

Friedmann

et al. 2019

Journal Orthopaedic Research

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The focus of this work is to assess the long‐term progression of degeneration in the ovine lumbar spine following a minimally invasive model injury comparable to the damage of an intervertebral disc (IVD) herniation. A partial nucleotomy was performed on 18 sheep via the percutaneous dorsolateral approach. The animals were culled at 6 and 12 months to evaluate the damaged and neighboring functional spine units (FSUs) for degenerative characteristics via μ‐CT and histology. Both quantitative μ‐CT and histology investigations demonstrated statistically significant differences between the native and damaged FSUs investigated. Qualitative analysis of μ‐CT revealed numerous pathological markers consistent with intervertebral disc degeneration (IDD), with differences in frequency and severity between the native and damaged FSUs. The annulus fibrosus reforms a pressure seal within 6 weeks, but the extent of the trauma is significant enough to initiate IVD degeneration, which is already clearly visible at 6 months and especially so 12 months post‐op. IDD pathology consistent with signs of a herniation was seen in both the 6‐ and 12‐month groups. This technique provides a useful model injury for the preclinical evaluation of IDD in large animal models, especially in regards to simulating disc herniation as well as for testing the efficacy of associated therapies in the future. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2376–2388, 2019

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Section: Resultsmentioning

confidence: 96%

“…Figure shows the change in IVD height at nine different points for both groups according to a previously described method . In Group 1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

Schwan

Ludtka

Friedmann

et al. 2019

Journal Orthopaedic Research

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A comprehensive tool box for large animal studies of intervertebral disc degeneration

et al. 2021

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Preclinical studies involving large animal models aim to recapitulate the clinical situation as much as possible and bridge the gap from benchtop to bedside. To date, studies investigating intervertebral disc (IVD) degeneration and regeneration in large animal models have utilized a wide spectrum of methodologies for outcome evaluation. This paper aims to consolidate available knowledge, expertise, and experience in large animal preclinical models of IVD degeneration to create a comprehensive tool box of anatomical and functional outcomes. Herein, we present a Large Animal IVD Scoring Algorithm based on three scales: macroscopic (gross morphology, imaging, and biomechanics), microscopic (histological, biochemical, and biomolecular analyses), and clinical (neurologic state, mobility, and pain). The proposed algorithm encompasses a stepwise evaluation on all three scales, including spinal pain assessment, and relevant structural and functional components of IVD health and disease. This comprehensive tool box was designed for four commonly used preclinical large animal models (dog, pig, goat, and sheep) in order to facilitate standardization and applicability. Furthermore, it is intended to facilitate comparison across studies while discerning relevant differences between species within the context of outcomes with the Naomi N. Lee and Elias Salzer, contributed equally (shared first authorship); Julie B. Engiles and Marianna A. Tryfonidou contributed equally (shared last authorship)

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Long‐term pre‐clinical evaluation of an injectable chitosan nanocellulose hydrogel with encapsulated adipose‐derived stem cells in an ovine model for IVD regeneration

Schmitt

Radetzki

Stirnweiss

et al. 2021

J Tissue Eng Regen Med

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The potential therapeutic benefit of adipose‐derived stem cells (ASCs) encapsulated in an injectable hydrogel for stimulating intervertebral disc (IVD) regeneration has been assessed by a number of translational and preclinical studies. However, previous work has been primarily limited to small animal models and short‐term outcomes of only a few weeks. Long‐term studies in representative large animal models are crucial for translation into clinical success, especially for permanent stabilization of major defects such as disc herniation. An injectable chitosan carboxymethyl cellulose hydrogel scaffold loaded with ASCs was evaluated regarding its intraoperative handling, crosslinking kinetics, cell viability, fully‐crosslinked viscoelasticity, and long‐term therapeutic effects in an ovine model. Three IVDs per animal were damaged in 10 sheep. Subcutaneous adipose tissue was the source for autologous ASCs. Six weeks after IVD damage, two of the damaged IVDs were treated via ASC‐loaded hydrogel injection. After 12 months following the implantation, IVD disc height and histological and cellular changes were assessed. This system was reliable and easy to handle intraoperatively. Over 12 months, IVD height was stabilized and degeneration progression significantly mitigated compared to untreated, damaged IVDs. Here we show for the first time in a large animal model that an injectable chitosan carboxymethyl cellulose hydrogel system with encapsulated ASCs is able to affect long‐term stabilization of an injured IVD and significantly decrease degeneration processes as compared to controls.

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Micro-CT evaluation of asymmetrical ovine intervertebral disc height loss from surgical approach

Cited by 8 publications

References 24 publications

Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

A comprehensive tool box for large animal studies of intervertebral disc degeneration

Long‐term pre‐clinical evaluation of an injectable chitosan nanocellulose hydrogel with encapsulated adipose‐derived stem cells in an ovine model for IVD regeneration

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