Micro-CT scan, electron microscopy and optical microscopy study of insertional traumas of cochlear implants

Breton, Alexia Le; Jégoux, Franck; Pilet, Paul; Godey, Benoît

doi:10.1007/s00276-015-1469-9

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…Earlier studies have shown that micro-CT is feasible for in vitro electrode studies (15)(16)(17). Whereas Le Breton et al (15) concluded that micro-CT is not sufficiently accurate for the reliable assessment of insertion trauma, Postnov et al (17) found nearly equal visualization of the cochlear structures and the electrode array as in histological samples. Similar to our study, Postnov et al removed the perilymph to increase the contrast of the BM and better visualization.…”

Section: Discussionmentioning

confidence: 99%

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Trauma After Cochlear Implantation: The Accuracy of Micro–Computed Tomography and Cone-Beam Fusion Computed Tomography Compared With Histology in Human Temporal Bones

Iso-Mustajärvi¹,

Silvast²,

Heikka³

et al. 2023

Otol Neurotol

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HypothesisMicro-computed tomography (micro-CT) and cone-beam computed tomography (CBCT), in conjunction with the image fusion technique, may provide similar results for trauma assessment after cochlear implantation, with respect to the trauma evaluation in preclinical cochlear implant (CI) studies, as the histology.BackgroundBefore clinical use, novel cochlear implant (CI) designs are tested in temporal bone (TB) studies for usability and risk evaluation. The criterion standard for evaluating intracochlear insertion trauma and electrode location has historically been with histological samples. Progress of modern imaging technology has created alternatives to classic histology. This study compares the micro-CT and CBCT fusion images between histological samples in a preclinical CI study.MethodsFourteen freshly frozen TBs were inserted with a lateral wall research CI electrode. All TBs were scanned with CBCT preoperatively and postoperatively. After insertion, the TBs were prepared for micro-CT and histology. Twelve TBs underwent first a micro-CT and then the histological process. The CBCTs were used for image fusion, and all three different methods were used for intracochlear trauma evaluation. The results were compared between methods.ResultsThere were 4 of 14 translocations detected with the fusion image method and 3 of 12 with the micro-CT and histology. When compared, the trauma grades converged and were not statistically significant.ConclusionThe trauma grading based on micro-CT is comparable to the histology. The image fusion technique based on CBCT is less accurate because it relies on an empirical assumption of the basal membrane localization, but it is clinically applicable.

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Section: Discussionmentioning

confidence: 99%

“…Micro-computed tomography (micro-CT) is another feasible imaging modality used for the investigation of cochlear trauma in TB studies (15)(16)(17). It can provide highly detailed images of the cochlea, it is significantly less time-consuming, and it requires no manipulation when compared with histology.…”

Section: Introductionmentioning

confidence: 99%

Trauma After Cochlear Implantation: The Accuracy of Micro–Computed Tomography and Cone-Beam Fusion Computed Tomography Compared With Histology in Human Temporal Bones

Iso-Mustajärvi¹,

Silvast²,

Heikka³

et al. 2023

Otol Neurotol

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“…The authors claim that a combination of micro-CT and optical microscopy is necessary to determine the spatial relationships of vessels with different diameters. In medical studies, the combining approach has been proven very efficient to detect cochlear trauma caused by cochlear implantation [ 45 ]. The study concludes that a combination of 2D imaging, 3D imaging, and histology is necessary in order to have reliable results regarding the trauma.…”

Section: Imaging Techniquesmentioning

confidence: 99%

Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques

et al. 2021

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Several imaging techniques are used in biological and biomedical studies. Micro-computed tomography (micro-CT) is a non-destructive imaging technique that allows the rapid digitisation of internal and external structures of a sample in three dimensions and with great resolution. In this review, the strengths and weaknesses of some common imaging techniques applied in biological and biomedical fields, such as optical microscopy, confocal laser scanning microscopy, and scanning electron microscopy, are presented and compared with the micro-CT technique through five use cases. Finally, the ability of micro-CT to create non-destructively 3D anatomical and morphological data in sub-micron resolution and the necessity to develop complementary methods with other imaging techniques, in order to overcome limitations caused by each technique, is emphasised.

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“…been reported to provide a resolution of around 10 to 30 µm pixel size (Le Breton et al 2015;Malherbe et al 2013;Voie et al 1993) within the context of cochlear imaging.…”

Section: Opfos An Optical Sectioning Technique and μCt That Is A Radmentioning

confidence: 99%

Three-dimensional models of cochlear implants: A review of their development and how they could support management and maintenance of cochlear implant performance

Hanekom

2016

Network: Computation in Neural Systems

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Three-dimensional (3D) computational modeling of the auditory periphery forms an integral part of modern-day research in cochlear implants (CIs). These models consist of a volume conduction description of implanted stimulation electrodes and the current distribution around these, coupled with auditory nerve fiber models. Cochlear neural activation patterns can then be predicted for a given input stimulus. The objective of this article is to present the context of 3D modeling within the field of CIs, the different models, and approaches to models that have been developed over the years, as well as the applications and potential applications of these models. The process of development of 3D models is discussed, and the article places specific emphasis on the complementary roles of generic models and user-specific models, as the latter is important for translation of these models into clinical application.

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Micro-CT scan, electron microscopy and optical microscopy study of insertional traumas of cochlear implants

Abstract: In our opinion, the combination of 3D micro-CT scan reconstructions and histological analysis using OM appears to be the best method to analyze this type of trauma.

Cited by 9 publications

References 25 publications

Trauma After Cochlear Implantation: The Accuracy of Micro–Computed Tomography and Cone-Beam Fusion Computed Tomography Compared With Histology in Human Temporal Bones

Trauma After Cochlear Implantation: The Accuracy of Micro–Computed Tomography and Cone-Beam Fusion Computed Tomography Compared With Histology in Human Temporal Bones

Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques

Three-dimensional models of cochlear implants: A review of their development and how they could support management and maintenance of cochlear implant performance

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