In this study, a fiber push-out device based on a piezoelectric actuator was designed, analyzed and tested, and its experimental environment was designed. The piezoelectric actuator includes a flexible beam. By using response surface analysis (RSM), taking the large displacement as the objective function and on the premise of meeting the strength requirements, the structural parameters of the flexible beam were analyzed. In the process of fiber push-out, the interfacial shear stress was estimated by establishing the system integrating the fiber-matrix-composite three-phase model and the piezoelectric actuator model using the analytic method, and the theoretical analysis results of the fiber interface mechanical properties were given. A prototype of the system was made, and the performance tests of the piezoelectric actuator and the fiber push-out device were carried out. The test results showed that the designed piezoelectric actuator can achieve a stepping resolution of 6.67 μm and a maximum displacement of about 100 μm at the input voltage of 150 V, which is consistent with the design results. The extrusion test of a single fiber was carried out using a piezoelectric actuator. The mechanical properties of the interfacial layer during the push-out process were measured and the interfacial shear strength was calculated, which is consistent with the theoretical analysis results. Finally, based on the mechanical properties obtained from the test, the loading failure process of the fiber was simulated by finite element analysis, which well explained the failure process of the fiber, thus verifying the feasibility of the designed fiber push-out device.