Micro-electrode channel guide (µECG) technology: an online method for continuous electrical recording in a human beating heart-on-chip

Visone, Roberta; Ugolini, Giovanni Stefano; Cruz-Moreira, Daniela; Marzorati, Simona; Piazza, Stefano; Pesenti, Enrico; Redaelli, Alberto; Moretti, Matteo; Occhetta, Paola; Rasponi, Marco

doi:10.1088/1758-5090/abe4c4

Cited by 26 publications

(40 citation statements)

References 63 publications

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“…Moreover, the incorporation of sensing electrodes to record online the field potential generated by cardiac microtissues demonstrated how the mechanical stimulation gradually improves the electrical maturation of the constructs, which showed an increased synchronicity and a lower variation of the beating frequency after 5 days in dynamic culture conditions. The system was also demonstrated suitable to perform drug cardiotoxicity screening, by monitoring the electric field alteration caused by compounds in both murine and hiPSC-CM with human fibroblasts (Visone et al 2021).…”

Section: Active Stimulationmentioning

confidence: 99%

Current strategies of mechanical stimulation for maturation of cardiac microtissues

et al. 2021

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The most advanced in vitro cardiac models are today based on the use of induced pluripotent stem cells (iPSCs); however, the maturation of cardiomyocytes (CMs) has not yet been fully achieved. Therefore, there is a rising need to move towards models capable of promoting an adult-like cardiomyocytes phenotype. Many strategies have been applied such as co-culture of cardiomyocytes, with fibroblasts and endothelial cells, or conditioning them through biochemical factors and physical stimulations. Here, we focus on mechanical stimulation as it aims to mimic the different mechanical forces that heart receives during its development and the post-natal period. We describe the current strategies and the mechanical properties necessary to promote a positive response in cardiac tissues from different cell sources, distinguishing between passive stimulation, which includes stiffness, topography and static stress and active stimulation, encompassing cyclic strain, compression or perfusion. We also highlight how mechanical stimulation is applied in disease modelling.

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Section: Active Stimulationmentioning

confidence: 99%

Current strategies of mechanical stimulation for maturation of cardiac microtissues

et al. 2021

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“…Specifically, this chip fits the bottom chamber described by Marsano et al, and allows the user to introduce uniaxial mechanical stimulation on demand (Marsano et al, 2016 ). Moreover, guiding channels can be easily added to the chip geometry to position microelectrodes and provide either electrical stimulation (Visone et al, 2018 ) or online continuous electrical recording (Visone et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Assessing the influence of perfusion on cardiac microtissue maturation: A heart‐on‐chip platform embedding peristaltic pump capabilities

Cruz-Moreira

Visone

Vasques‐Nóvoa

et al. 2021

Biotech & Bioengineering

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Heart-on-chip is an unprecedented technology for recapitulating key biochemical and biophysical cues in cardiac pathophysiology. Several designs have been proposed to improve its ability to mimic the native tissue and establish it as a reliable research platform. However, despite mimicking one of most vascularized organs, reliable strategies to deliver oxygen and substrates to densely packed constructs of metabolically demanding cells remain unsettled. Herein, we describe a new hearton-chip platform with precise fluid control, integrating an on-chip peristaltic pump, allowing automated and fine control over flow on channels flanking a 3D cardiac culture. The application of distinct flow rates impacted on temporal dynamics of microtissue structural and transcriptional maturation, improving functional performance. Moreover, a widespread transcriptional response was observed, suggesting flow-mediated activation of critical pathways of cardiomyocyte structural and functional maturation and inhibition of cardiomyocyte hypoxic injury. In conclusion, the present design represents an important advance in bringing engineered cardiac microtissues closer to the native heart, overcoming traditional bulky off-chip fluid handling systems, improving microtissue performance, and matching oxygen and energy substrate requirements of metabolically active constructs, avoiding cellular hypoxia. Distinct flow patterns differently impact on microtissue performance and gene expression program.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…To overcome this issue, electrode guides bringing them in direct proximity of the cultured constructs might be introduced in the device as recently published. 45 Finally, we also tested the resistance of the high-CM population to fibrosis induction when both cyclical stretching was provided, and the profibrotic factor TGF-β1 was administered. Both immunofluorescence and RT-qPCR revealed that the high-CM condition seemed to resist the onset of a fibrotic phenotype with only a slight increase in fibrosis associated gene expression.…”

Section: Lab On a Chip Papermentioning

confidence: 99%

A dynamic microscale mid-throughput fibrosis model to investigate the effects of different ratios of cardiomyocytes and fibroblasts

et al. 2021

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Micro-electrode channel guide (µECG) technology: an online method for continuous electrical recording in a human beating heart-on-chip

Abstract: Micro-electrode channel guide (µECG) technology: an online method for continuous electrical recording in a human beating heart-on-chip To cite this article: Roberta Visone et al 2021 Biofabrication 13 035026 View the article online for updates and enhancements.

Cited by 26 publications

References 63 publications

Current strategies of mechanical stimulation for maturation of cardiac microtissues

Current strategies of mechanical stimulation for maturation of cardiac microtissues

Assessing the influence of perfusion on cardiac microtissue maturation: A heart‐on‐chip platform embedding peristaltic pump capabilities

A dynamic microscale mid-throughput fibrosis model to investigate the effects of different ratios of cardiomyocytes and fibroblasts

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