Micro extraction by packed sorbent coupled to liquid chromatography tandem mass spectrometry for the rapid and sensitive determination of cannabinoids in oral fluids

Impact of oral fluid collection device on cannabinoid stability following smoked cannabis

“…However, less device buffer results in lower total sample volume; which may be problematic in workplace, clinical and forensic drug testing settings where screening and multiple drug confirmation assays on the same specimen are commonplace. Other studies evaluated THC stability in fortified OF [18; 28; 29,30]. Wille et al [18] documented THC stability in fortified OF specimens collected with the StatSure device.…”

Techniques and Technologies for The Bioanalysis of Sativex ® , Metabolites and Related Compounds

“…A number of methods to simultaneously analyze multiple cannabinoids including both THC and CBD in oral fluid have recently been published. These methods utilize GC-MS [66][67][68], 2D-GC-MS [69] and LC-MS/MS [37,68,[70][71][72]. SPE is the most common form of sample preparation, however modified versions including solid-phase microextraction (SPME) [68] and micro-extraction by packed sorbent (MEPS) [72] have also been used, as well as LLE [37,71].…”

“…LOQs of THC and CBD were 0.5-2 and 0.9-2 ng/ml, respectively, when analyzed by GC-MS [66][67][68]. Using LC-MS/MS, LOQs were 0.25-1 ng/ml for THC and 0.3-2 ng/ml for CBD [37,[71][72][73]. Milman et al achieved a 0.5 ng/ml LOQ for both THC and CBD collected by the Quantisal device and analyzed using 2D-GC-MS [69].…”

“…Plasma GC-MS 0.080 (THC); 0.95 (CBD); 3.9 (CBN); 0.5 (11-OH-THC); 0.88 (THC-COOH) [38] GC-MS 0.1 (THC, CBD, 11-OH-THC) [26] 2D-GC-MS 0.25 (THC, CBD, THC-COOH); 0.125 (11-OH-THC) [43] Whole blood LC-MS/MS 2 (THC); 3 (CBD, 11-OH-THC, THC-COOH) [44] LC-MS/MS 1.0 (THC, CBD, CBN, 11-OH-THC, THC-COOH); 0.5 (THC-glu); 5.0 (THC-COOH-glu) [45] Urine GC-MS 3.4 (THC); 3.9 (CBD); 0.9 (THC-COOH) [51] LC-MS 2 (THC, THC-COOH) [46] LC-MS/MS 1 (THC, CBD, THC-COOH) [53] LC-MS/MS 0.5 (THC-glu); 5 (THC-COOH-glu); 1 (CBD, THC-COOH); 2 (THC) [50] LC-MS/MS 0.008 (THC, CBN); 0.012 (CBD); 0.018 (THC-COOH); 0.028 (11-OH-THC) [54] Oral fluid GC-MS 0.5 (THC, CBN); 1 (CBD, THC-A) [66] GC-MS 1.9 (THC); 0.9 (CBD); 4.8 (THC-COOH); 12.7 (11-OH-THC); 5.6 (CBN) [67] SPME-GC-MS 2 (THC, CBD, CBN) [68] LC-MS/MS 1 (THC); 2 (CBD, CBN) [37] LC-MS/MS 0.5 (THC, CBD, CBN, 11-OH-THC, THC-A); 0.08 (THC-COOH) [71] LC-MS/MS 0.25 (THC); 0.3 (CBD, CBN); 0.02 (THC-COOH); 0.4 (11-OH-THC) [72] LC-MS/MS 1 (THC) [73] 2D-GC-MS 0.5 (THC, CBD) [69] Microflow LC-MS/MS 0.5 (THC, CBD) [70] LC-MS/MS 0.25 (THC, CBD) [74] Hair GC-MS 50 pg/mg (THC, CBD, CBN) [81] GC-MS/MS 1 pg/mg (THC); 0.1 pg/mg (THC-COOH) [86] Headspace SPME-GC-MS/MS 62 pg/mg (THC); 12 pg/mg (CBD); 30 pg/mg (CBN) [76] LC-MS/MS 0.2 pg/mg (THC-COOH); 50 pg/mg (THC, CBD, CBN) [78] LC-MS/MS 3.9 pg/mg (THC); 18 pg/mg (CBD); 5.3 pg/mg (CBN) [84] LC-MS/MS 0.1 pg/mg (THC-COOH); 1.0 pg/mg (THC); 2.0 pg/mg (CBD, CBN) [87] Sweat GC-NCI-MS 0.4 ng/patch (THC) [92] Exhaled breath LC-MS/MS 6 pg/filter (THC) [95] † LOQ measurements given in ng/ml unless otherwise stated.…”

Dépistage de la consommation de substances psychoactives en milieu professionnel : enjeux techniques, éthiques et réglementaires

“…Citons enfin l'utilisation de spots de sang séchés, obtenus par une simple piqûre au bout du doigt, faciles à transporter et à conserver, et dont l'analyse par des techniques chromatographiques couplées à la spectrométrie de masse en tandem donne des résultats très prometteurs [21]. L'utilisation de la salive, longtemps controversée du fait du risque élevé de faux positifs pour les cannabinoïdes, a béné-ficié de progrès technologiques récents et sa fiabilité est meilleure actuellement [22,23]. Elle est d'ailleurs largement utilisée par les forces de l'ordre dans le dépistage de l'usage de stupéfiants chez les conducteurs de véhicules impliqués dans un accident de la circulation [24].…”